IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Identification of antigens by monoclonal antibody PD4 and its expression in Escherichia coli
Ning, JY; Sun, GX; Huang, S; Ma, H; An, P; Meng, L; Song, SM; Wu, L; Shou, CC
刊名WORLD JOURNAL OF GASTROENTEROLOGY
2003-10-01
9期:10页:2164-2168
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]HELICOBACTER-PYLORI INFECTION ; MOUSE SARCOMA-CELLS ; GASTRIC-CANCER ; MYCOPLASMA-INFECTIONS ; FUJIAN PROVINCE ; MALIGNANT-TRANSFORMATION ; CARCINOMA ; RISK ; GENE ; P53
英文摘要

AIM: To clone and express the antigen of monoclonal antibody (MAb) PD4 for further investigation of its function.

METHODS: MGC803 cDNA expression library was constructed and screened with PD4 as probes to clone the antigen. After failed in the library screening, immunoprecipitation and SDS-polyacrylamide gel electrophoresis were applied to purify the antigen for sequence analysis. The antigen coming from Mycoplasma hyorhinis (M. hyorhinis) was further confirmed with Western blot analysis by infecting M. hyorhinis -free HeLa cells and eliminating the M. hyorhinis from MGC803 cells. The full p37 gene was cloned by PCR and expressed successfully in Escherichia coli after site-directed mutations. Immunofluorescence assay was used to demonstrate if p37 protein could directly bind to gastric tumor cell AGS.

RESULTS: The cDNA library constructed with MGC803 cells was screened by MAb PD4 as probes. Unfortunately, the positive clones identified with MAb PD4 were also reacted with unrelated antibodies. Then, immunoprecipitation was performed and the purified antigen was identified to be a membrane protein of Mycoplasma hyorhinis (M. hyorhinis) by sequencing of N-terminal amino acid residues. The membrane protein was intensively verified with Western blot by eliminating M. hyorhinis from MGC803 cells and by infecting M. hyorhinis-free HeLa cells. The full p37 gene was cloned and expressed successfully in Escherichia coli after site-directed mutations. Immunofluorescence demonstrated that p37 protein could directly bind to gastric tumor cell AGS.

CONCLUSION: The antigen recognized by MAb PD4 is from M. hyorhinis, which suggests the actions involved in MAb PD4 is possibly mediated by p37 protein or M. hyorhinis. As p37 protein can bind directly to tumor cells, the pathogenic role of p37 involved in tumorigenesis justifies further investigation.

语种英语
WOS记录号WOS:000186055100008
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57338
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China
2.Peking Univ, Beijing Inst Canc Res, Beijing 100034, Peoples R China
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GB/T 7714
Ning, JY,Sun, GX,Huang, S,et al. Identification of antigens by monoclonal antibody PD4 and its expression in Escherichia coli[J]. WORLD JOURNAL OF GASTROENTEROLOGY,2003,9(10):2164-2168.
APA Ning, JY.,Sun, GX.,Huang, S.,Ma, H.,An, P.,...&Shou, CC.(2003).Identification of antigens by monoclonal antibody PD4 and its expression in Escherichia coli.WORLD JOURNAL OF GASTROENTEROLOGY,9(10),2164-2168.
MLA Ning, JY,et al."Identification of antigens by monoclonal antibody PD4 and its expression in Escherichia coli".WORLD JOURNAL OF GASTROENTEROLOGY 9.10(2003):2164-2168.
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