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学科主题: 临床医学
题名:
The "time-window" effect of early allergen exposure on a rat asthma model
作者: Hu Yan1; Liu Ping1; Li Hai-chao1; Wang Yue-dan2
关键词: asthma ; allergen exposure ; regulatory T cells ; costimulatory molecular
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2013-06-20
DOI: 10.3760/cma.j.issn.0366-6999.20122326
卷: 126, 期:12, 页:2265-2269
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: REGULATORY T-CELLS ; COSTIMULATORY MOLECULES ; AIRWAY HYPERREACTIVITY ; PERIPHERAL HOMEOSTASIS ; NEONATAL TOLERANCE ; CD4(+)CD25(+) ; INDUCTION ; ANTIGEN ; FAMILY ; FOXP3
英文摘要:

Background The hygiene hypothesis has been proposed to explain the pathogenesis of asthma. Allergen exposure was shown to inhibit asthma in an animal model. But the optimal timing of allergen exposure remains unclear. This study aims to explore the time effcct of allergen exposure and the possible mechanisms.

Methods Neonate Wistar rats were randomly divided into asthma group, control group and day 1, day 3, day 7, and day 14 groups. The day 1, day 3, day 7 and day 14 groups were injected with ovalbumin (OVA) subcutaneously on days 1, 3, 7 and 14 after birth, respectively. Six weeks later, all groups, except the control group, were sensitized and stimulated with OVA to make the asthma model. We observed the pulmonary pathologic changes, detected the regulatory T cells, and CD28 expression level in thymus and spleen by flow cytometry.

Results The asthmatic inflammation in the day 1,"day 3 and day 7 groups, but not the day 14 group, was alleviated. The asthma group and day 14 group had lower proportions of regulatory T cells in the thymus compared with the control group, day 1, day 3, and day 7 groups. There was no significant difference in the CD28 expression levels on the regulatory and conventional T cells among groups. But the control group and the day 1, day 3, and day 7 groups had relatively higher proportions of CD28 positive regulatory T cells in the thymus than the day 14 group and the asthma group.

Conclusions There is a "time-window" for early allergen exposure. The impairment of regulatory T cells may promote the development of asthma. Allergen exposure in the "time-window" can make the thymus produce normal quantity of regulatory cells. The CD28 signal on regulatory T cells may participate in the production of regulatory T cells.

语种: 英语
所属项目编号: 20110001120044
项目资助者: New Teachers&prime ; Fund for Doctor Stations of Ministry of Education of China
WOS记录号: WOS:000321473800012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57345
Appears in Collections:北京大学第一临床医学院_呼吸和危重症医学科_期刊论文

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作者单位: 1.Peking Univ, Dept Pulm Med, Hosp 1, Beijing 100034, Peoples R China
2.Peking Univ, Div Immunol, Hlth Sci Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Hu Yan,Liu Ping,Li Hai-chao,et al. The "time-window" effect of early allergen exposure on a rat asthma model[J]. CHINESE MEDICAL JOURNAL,2013,126(12):2265-2269.
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