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学科主题基础医学
The evidences of human Orphan Receptor COUP-TFII inhibiting telomerase activity through decreasing hTERT transcription
Wang, Q; Bai, ZL; Li, X; Hou, L; Zhang, B
关键词yeast one-hybrid assay COUP-TFII hTERT telomerase transcription regulation
刊名CANCER LETTERS
2004-10-08
DOI10.1016/j.canlet.2004.03.032
214期:1页:81-90
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CANCER CELL-LINES ; STEROID-RECEPTOR ; GENE-EXPRESSION ; ACTIVATES TELOMERASE ; CATALYTIC SUBUNIT ; PROMOTER ; SUPERFAMILY ; IDENTIFICATION ; REPRESSION ; CLONING
英文摘要

Activation of hTERT, the human telomerase catalytic subunit, has been implicated as the critical event in triggering telomerase activity of cancer cells, but the details of regulation of hTERT need to be elucidated. By screening HeLa cDNA library with hTERT promoter-based yeast one-hybrid assay, one of positive clones, which potentially interacted with hTERT promoter, contained the full sequences of COUP-TFII cDNA. EMSA showed that the prepared His-tagged COUP-TFII could firmly bind to -201 to +35 fragment of hTERT promoter. The precise binding sites were confirmed by DNase I footprint analysis and proved to involve the E-box motif of hTERT promoter. Luciferase reporter assays indicated that COUP-TFII could suppress the transcription of hTERT promoter, and the inhibition to some extent could be reversed by co-transfection of c-myc. Stable introduction of COUP-TFII into HeLa cells decreased both endogenous hTERT expression and telomerase activity. The results suggested that the human COUP-TFII could specifically interact with hTERT promoter, in part via the E-box, and suppress the expression of hTERT. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000224145500009
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57353
专题北京大学基础医学院_病理学系
作者单位1.Peking Univ, Dept Pathol, Ctr Hlth Sci, Beijing 100083, Peoples R China
2.Shandong Univ, Prov Key Lab Mechanisms Dev & Gene Control, Sch Life Sci, Jinan 250100, Peoples R China
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GB/T 7714
Wang, Q,Bai, ZL,Li, X,et al. The evidences of human Orphan Receptor COUP-TFII inhibiting telomerase activity through decreasing hTERT transcription[J]. CANCER LETTERS,2004,214(1):81-90.
APA Wang, Q,Bai, ZL,Li, X,Hou, L,&Zhang, B.(2004).The evidences of human Orphan Receptor COUP-TFII inhibiting telomerase activity through decreasing hTERT transcription.CANCER LETTERS,214(1),81-90.
MLA Wang, Q,et al."The evidences of human Orphan Receptor COUP-TFII inhibiting telomerase activity through decreasing hTERT transcription".CANCER LETTERS 214.1(2004):81-90.
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