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学科主题: 基础医学
题名:
The evidences of human Orphan Receptor COUP-TFII inhibiting telomerase activity through decreasing hTERT transcription
作者: Wang, Q; Bai, ZL; Li, X; Hou, L; Zhang, B
关键词: yeast one-hybrid assay ; COUP-TFII ; hTERT ; telomerase ; transcription regulation
刊名: CANCER LETTERS
发表日期: 2004-10-08
DOI: 10.1016/j.canlet.2004.03.032
卷: 214, 期:1, 页:81-90
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CANCER CELL-LINES ; STEROID-RECEPTOR ; GENE-EXPRESSION ; ACTIVATES TELOMERASE ; CATALYTIC SUBUNIT ; PROMOTER ; SUPERFAMILY ; IDENTIFICATION ; REPRESSION ; CLONING
英文摘要:

Activation of hTERT, the human telomerase catalytic subunit, has been implicated as the critical event in triggering telomerase activity of cancer cells, but the details of regulation of hTERT need to be elucidated. By screening HeLa cDNA library with hTERT promoter-based yeast one-hybrid assay, one of positive clones, which potentially interacted with hTERT promoter, contained the full sequences of COUP-TFII cDNA. EMSA showed that the prepared His-tagged COUP-TFII could firmly bind to -201 to +35 fragment of hTERT promoter. The precise binding sites were confirmed by DNase I footprint analysis and proved to involve the E-box motif of hTERT promoter. Luciferase reporter assays indicated that COUP-TFII could suppress the transcription of hTERT promoter, and the inhibition to some extent could be reversed by co-transfection of c-myc. Stable introduction of COUP-TFII into HeLa cells decreased both endogenous hTERT expression and telomerase activity. The results suggested that the human COUP-TFII could specifically interact with hTERT promoter, in part via the E-box, and suppress the expression of hTERT. (C) 2004 Elsevier Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000224145500009
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57353
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.Peking Univ, Dept Pathol, Ctr Hlth Sci, Beijing 100083, Peoples R China
2.Shandong Univ, Prov Key Lab Mechanisms Dev & Gene Control, Sch Life Sci, Jinan 250100, Peoples R China

Recommended Citation:
Wang, Q,Bai, ZL,Li, X,et al. The evidences of human Orphan Receptor COUP-TFII inhibiting telomerase activity through decreasing hTERT transcription[J]. CANCER LETTERS,2004,214(1):81-90.
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