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17-beta-Estradiol Enhanced Allodynia of Inflammatory Temporomandibular Joint through Upregulation of Hippocampal TRPV1 in Ovariectomized Rats
Wu, Yu-Wei1; Bi, Ye-Ping2,3; Kou, Xiao-Xing1; Xu, Wen2,3; Ma, Li-Qun4; Wang, Ke-Wei2,3; Gan, Ye-Hua1; Ma, Xu-Chen1
刊名JOURNAL OF NEUROSCIENCE
2010-06-30
DOI10.1523/JNEUROSCI.6323-09.2010
30期:26页:8710-8719
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]SYNAPTIC PLASTICITY ; DENTATE GYRUS ; PERIAQUEDUCTAL GRAY ; RECEPTOR EXPRESSION ; NOXIOUS-STIMULATION ; ESTROUS-CYCLE ; PLASMA-LEVELS ; FORMALIN TEST ; SEX-HORMONES ; FEMALE RATS
英文摘要

Temporomandibular disorders (TMDs) predominantly affect reproductive female patients, with pain the most frequent complaint. Although estrogens are believed to play important roles in TMD pain, the mechanism underlying modulation of TMD pain by estrogens remains largely unknown. Accumulating evidence implies that the hippocampus is involved in sexual dimorphism of pain sensitivity. In this study, we investigated the hippocampal TRPV1 (transient receptor potential vanilloid 1) expression in ovariectomized rats that received 17-beta-estradiol substitution and found that 17-beta-estradiol enhanced the mechanical allodynia of inflamed temporomandibular joint (TMJ) induced by complete Freund′s adjuvant. Real-time PCR and immunoblotting demonstrated that TMJ inflammation significantly induced hippocampal TRPV1 expression compared with the control group but failed to induce it in the ovariectomized rats that received no estradiol replacement. In addition, estradiol potentiated TMJ inflammation-induced hippocampal TRPV1 expression in a dose-dependent manner in the ovariectomized rats. In contrast, TRPV1 transcription in amygdala, prefrontal cortex, and thalamus was not affected by TMJ inflammation and estradiol. Immunostaining showed TRPV1 localized in the processes and cytoplasm of pyramidal neurons in CA1-CA3 regions of the hippocampus. Moreover, intrahippocampal injection of TRPV1 antagonists capsazepine and 5′-iodo-resiniferatoxin into the CA1 region of the hippocampus significantly attenuated allodynia of inflamed TMJ in both nonovariectomized and ovariectomized rats that received estradiol replacement. Our results suggested that hippocampal TRPV1 can modulate central pain processing and estradiol may contribute to the sexual dimorphism of TMD pain sensitivity through upregulation of TRPV1 expression in the hippocampus.

语种英语
所属项目编号30740046 ; 30970919
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000279581500004
引用统计
被引频次:45[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57357
专题基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100081, Peoples R China
3.Peking Univ, Minist Educ & Hlth, Key Lab Neurosci, Dept Neurobiol, Beijing 100191, Peoples R China
4.Third Mil Med Univ, Daping Hosp, Dept Hypertens & Endocrinol, Chongqing 400042, Peoples R China
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Wu, Yu-Wei,Bi, Ye-Ping,Kou, Xiao-Xing,et al. 17-beta-Estradiol Enhanced Allodynia of Inflammatory Temporomandibular Joint through Upregulation of Hippocampal TRPV1 in Ovariectomized Rats[J]. JOURNAL OF NEUROSCIENCE,2010,30(26):8710-8719.
APA Wu, Yu-Wei.,Bi, Ye-Ping.,Kou, Xiao-Xing.,Xu, Wen.,Ma, Li-Qun.,...&Ma, Xu-Chen.(2010).17-beta-Estradiol Enhanced Allodynia of Inflammatory Temporomandibular Joint through Upregulation of Hippocampal TRPV1 in Ovariectomized Rats.JOURNAL OF NEUROSCIENCE,30(26),8710-8719.
MLA Wu, Yu-Wei,et al."17-beta-Estradiol Enhanced Allodynia of Inflammatory Temporomandibular Joint through Upregulation of Hippocampal TRPV1 in Ovariectomized Rats".JOURNAL OF NEUROSCIENCE 30.26(2010):8710-8719.
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