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Dopamine D-1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells
Xu, Jiao-jiao1,3; Wang, Si-yuan1; Chen, Ye1; Chen, Guang-ping2; Li, Zai-quan4; Shao, Xue-yan1; Li, Liang1; Lu, Wei1; Zhou, Tian-yan1
关键词Dopamine D-1 Receptor Dehydroepiandrosterone Sulfotransferase (Sult2a1) Drug-metabolizing Enzyme Skf82958 Skf38393 Sch23390 Sirna Hepg2 Cell
刊名ACTA PHARMACOLOGICA SINICA
2014-07-01
DOI10.1038/aps.2014.19
35期:7页:889-898
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]CYTOSOLIC SULFOTRANSFERASES ; LIVER CYTOCHROME-P450 ; EXPRESSION ; INDUCTION ; AGONISTS ; SYSTEM ; GENES ; NEUROTOXICITY ; HYPERTENSION ; INHIBITION
英文摘要

Aim: Dopamine receptors are present in the nervous system and also widely distributed in the periphery. The aim of this study was to investigate the role of D-1 subtype dopamine receptors (DRD1) in the regulation of dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells.

Methods: HepG2 cells were treated with DRD1 agonists with or without DRD1 antagonist for 9 d. DRD1 and SULT2A1 mRNA expression, protein expression, and SULT2A1 activity were detected using RT-PCR, Western blotting and HPLC, respectively. The level of cAMP was measured using a commercial kit.

Results: All the 5 DR subtypes (DRD1-DRD5) were found to be expressed in HepG2 cells. Treatment of HepG2 cells with the specific DRD1 agonists SKF82958 (2.5 mu mol/L) or SKF38393 (5 and 50 mu mol/L) significantly increased the mRNA and protein expression of both DRD1 and SULT2A1, and increased SULT2A1 activity and cAMP levels,. These effects were partially blocked by co-treatment with the specific DRD1 antagonist SCH23390 (2.5 mu mol/L). In addition, transfection of HepG2 cells with DRD1-specific siRNAs decreased DRD1 mRNA expression by 40%, which resulted in the reduction of SULT2A1 mRNA expression by 60%, protein expression by 40%, and enzyme activity by 20%.

Conclusion: DRD1 activation upregulates DRD1 and SULT2A1 expression and SULT2A1 activity in HepG2 cells, suggesting that the DRD1 subtype may be involved in the metabolism of drugs and xenobiotics through regulating SULT2A1.

语种英语
WOS记录号WOS:000338910500005
项目编号81072699 ; [2011]508
资助机构National Natural Science Foundation of China ; Scientific Research Foundation for the Returned Overseas Chinese Scholars by the State Education Ministry
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57371
专题北京大学药学院_药剂学系
北京大学医学部管理机构_医学部
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100191, Peoples R China
2.Oklahoma State Univ, Dept Physiol Sci, Ctr Vet Hlth Sci, Stillwater, OK 74078 USA
3.Zhejiang Prov Ctr Dis Control & Prevent, Lab Physicochem Res, Dept Physicochem & Toxicol, Hangzhou 310051, Zhejiang, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Pathol, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xu, Jiao-jiao,Wang, Si-yuan,Chen, Ye,et al. Dopamine D-1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells[J]. ACTA PHARMACOLOGICA SINICA,2014,35(7):889-898.
APA Xu, Jiao-jiao.,Wang, Si-yuan.,Chen, Ye.,Chen, Guang-ping.,Li, Zai-quan.,...&Zhou, Tian-yan.(2014).Dopamine D-1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells.ACTA PHARMACOLOGICA SINICA,35(7),889-898.
MLA Xu, Jiao-jiao,et al."Dopamine D-1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells".ACTA PHARMACOLOGICA SINICA 35.7(2014):889-898.
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