北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 药剂学系  > 期刊论文
学科主题: 药学
题名:
Dopamine D-1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells
作者: Xu, Jiao-jiao1,3; Wang, Si-yuan1; Chen, Ye1; Chen, Guang-ping2; Li, Zai-quan4; Shao, Xue-yan1; Li, Liang1; Lu, Wei1; Zhou, Tian-yan1
关键词: dopamine ; D-1 receptor ; dehydroepiandrosterone sulfotransferase (SULT2A1) ; drug-metabolizing enzyme ; SKF82958 ; SKF38393 ; SCH23390 ; siRNA ; HepG2 cell
刊名: ACTA PHARMACOLOGICA SINICA
发表日期: 2014-07-01
DOI: 10.1038/aps.2014.19
卷: 35, 期:7, 页:889-898
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: CYTOSOLIC SULFOTRANSFERASES ; LIVER CYTOCHROME-P450 ; EXPRESSION ; INDUCTION ; AGONISTS ; SYSTEM ; GENES ; NEUROTOXICITY ; HYPERTENSION ; INHIBITION
英文摘要:

Aim: Dopamine receptors are present in the nervous system and also widely distributed in the periphery. The aim of this study was to investigate the role of D-1 subtype dopamine receptors (DRD1) in the regulation of dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells.

Methods: HepG2 cells were treated with DRD1 agonists with or without DRD1 antagonist for 9 d. DRD1 and SULT2A1 mRNA expression, protein expression, and SULT2A1 activity were detected using RT-PCR, Western blotting and HPLC, respectively. The level of cAMP was measured using a commercial kit.

Results: All the 5 DR subtypes (DRD1-DRD5) were found to be expressed in HepG2 cells. Treatment of HepG2 cells with the specific DRD1 agonists SKF82958 (2.5 mu mol/L) or SKF38393 (5 and 50 mu mol/L) significantly increased the mRNA and protein expression of both DRD1 and SULT2A1, and increased SULT2A1 activity and cAMP levels,. These effects were partially blocked by co-treatment with the specific DRD1 antagonist SCH23390 (2.5 mu mol/L). In addition, transfection of HepG2 cells with DRD1-specific siRNAs decreased DRD1 mRNA expression by 40%, which resulted in the reduction of SULT2A1 mRNA expression by 60%, protein expression by 40%, and enzyme activity by 20%.

Conclusion: DRD1 activation upregulates DRD1 and SULT2A1 expression and SULT2A1 activity in HepG2 cells, suggesting that the DRD1 subtype may be involved in the metabolism of drugs and xenobiotics through regulating SULT2A1.

语种: 英语
所属项目编号: 81072699 ; [2011]508
项目资助者: National Natural Science Foundation of China ; Scientific Research Foundation for the Returned Overseas Chinese Scholars by the State Education Ministry
WOS记录号: WOS:000338910500005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57371
Appears in Collections:北京大学药学院_药剂学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100191, Peoples R China
2.Oklahoma State Univ, Dept Physiol Sci, Ctr Vet Hlth Sci, Stillwater, OK 74078 USA
3.Zhejiang Prov Ctr Dis Control & Prevent, Lab Physicochem Res, Dept Physicochem & Toxicol, Hangzhou 310051, Zhejiang, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Pathol, Beijing 100191, Peoples R China

Recommended Citation:
Xu, Jiao-jiao,Wang, Si-yuan,Chen, Ye,et al. Dopamine D-1 receptor activation induces dehydroepiandrosterone sulfotransferase (SULT2A1) in HepG2 cells[J]. ACTA PHARMACOLOGICA SINICA,2014,35(7):889-898.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Xu, Jiao-jiao]'s Articles
[Wang, Si-yuan]'s Articles
[Chen, Ye]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Xu, Jiao-jiao]‘s Articles
[Wang, Si-yuan]‘s Articles
[Chen, Ye]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace