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学科主题临床医学
Characterization of the promoter of 1A6/DRIM, a novel cancer-related gene and identification of its transcriptional activator
Xing, XY; Du, XJ; Lu, ZM; Ning, T; Su, XL; Ke, Y
关键词Transcriptional Regulation E-box Upstream Stimulatory Factor Tumorigenesis
刊名GENE
2005-01-03
DOI10.1016/j.gene.2004.09.036
344页:161-169
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Genetics & Heredity
研究领域[WOS]Genetics & Heredity
关键词[WOS]MAJOR LATE PROMOTER ; DNA-BINDING ; FACTOR USF ; IMMUNOGLOBULIN ENHANCER ; REGULATORY PROTEINS ; CELL-LINES ; C-MYC ; EXPRESSION ; UPSTREAM ; TARGET
英文摘要

1A6/DRIM (Down-regulated in Metastasis) has been reported to express at a high level in the gastric cancer tissues and the premalignant lesions implicating the involvement of IA6/DRIM in cell transformation. Although the information regarding the putative functions and distribution of the IA6/DRIM in different tissues and cell lines has been increasing recently, its promoter and promoterregulating factors remain unknown. In this study, the transcription initiation site of IA6/DRIM was confirmed to be located at 147 bp upstream of the ATG codon using the primer extension analysis. The minimal promoter region of the IA6/DRIM is located between -47 and +42 of the transcription initiation site measured by luciferase reporter assays using a set of deletion constructs. In addition, an E-box is shown to be an essential element for transcriptional regulation of IA6/DRIM demonstrated by luciferase assay with different deletion and mutation constructs. Finally, a transcription factor, upstream stimulatory factor 2 (USF2) was found to be an activator of the IA6/DRIM through binding to the E-box demonstrated by luciferase reporter assay, electrophoretic mobility shift assay, and chromatin immunoprecipitation (ChIP) assay. The structural analysis of the IA6/DRIM promoter and the identification of its potential regulatory effecter may help us to understand its biological functions in regulating cancer development. (C) 2004 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000226924600016
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57393
专题北京大学临床肿瘤学院
作者单位1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Inner Mongolia Med Coll, Hohhot 010059, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100034, Peoples R China
4.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100083, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Canc Res Ctr, Beijing 100083, Peoples R China
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GB/T 7714
Xing, XY,Du, XJ,Lu, ZM,et al. Characterization of the promoter of 1A6/DRIM, a novel cancer-related gene and identification of its transcriptional activator[J]. GENE,2005,344:161-169.
APA Xing, XY,Du, XJ,Lu, ZM,Ning, T,Su, XL,&Ke, Y.(2005).Characterization of the promoter of 1A6/DRIM, a novel cancer-related gene and identification of its transcriptional activator.GENE,344,161-169.
MLA Xing, XY,et al."Characterization of the promoter of 1A6/DRIM, a novel cancer-related gene and identification of its transcriptional activator".GENE 344(2005):161-169.
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