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学科主题: 药学
题名:
Mechanism for the TtDnaA-Tt-oriC cooperative interaction at high temperature and duplex opening at an unusual AT-rich region in Thermoanaerobacter tengcongensis
作者: Pei, Huadong; Liu, Jingfang; Li, Jie; Guo, Aobo; Zhou, Jian; Xiang, Hua
刊名: NUCLEIC ACIDS RESEARCH
发表日期: 2007-05-01
DOI: 10.1093/nar/gkm137
卷: 35, 期:9, 页:3087-3099
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: REPLICATION INITIATOR DNAA ; ESCHERICHIA-COLI ; CHROMOSOMAL ORIGIN ; SEQUENTIAL BINDING ; PROTEIN DNAA ; COMPLEX ; IDENTIFICATION ; HDA ; PURIFICATION ; ARCHITECTURE
英文摘要:

Thermoanaerobacter tengcongensis is an anaerobic low- GC thermophilic bacterium. To further elucidate the replication initiation of chromosomal DNA at high temperature, the interaction between the replication initiator ( TtDnaA) and the putative origin ( Tt-oriC) in this thermophile was investigated. We found that efficient binding of TtDnaA to Tt-oriC at high temperature requires ( i) at least two neighboring DnaA boxes, ( ii) the specific feature of the TtDnaA Domain IV and ( iii) the self-oligomerization of TtDnaA. Replacement of the TtDnaA Domain IV by the counterpart of Escherichia coli DnaA or disruption of its oligomerization by amino acid mutations ( W9A/L20S) abolished the oriC- binding activity of TtDnaA at 60 degrees C, but not at 37 degrees C. Moreover, ATP- TtDnaA, but not ADP- TtDnaA or the oligomerization- deficient mutants was able to unwind the Tt- oriC duplex. The minimal oriC required for this duplex opening in vitro was demonstrated to consist of DnaA boxes 1 - 8 and an unusual AT- rich region. Interestingly, although no typical ATP- DnaA box was found in this AT- rich region, it was exclusively bound by ATP- TtDnaA and acted as the duplex-opening and replication- initiation site. Taken together, we propose that oligomerization of ATP- DnaA and simultaneously binding of several DnaA boxes and/ or AT- rich region may be generally required in replication initiation at high temperature.

语种: 英语
WOS记录号: WOS:000247350800026
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内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57402
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Grad Univ Chinese Acad Sci, Beijing 100039, Peoples R China
2.Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100080, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Pei, Huadong,Liu, Jingfang,Li, Jie,et al. Mechanism for the TtDnaA-Tt-oriC cooperative interaction at high temperature and duplex opening at an unusual AT-rich region in Thermoanaerobacter tengcongensis[J]. NUCLEIC ACIDS RESEARCH,2007,35(9):3087-3099.
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