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Modulation of the Differentiation of Dental Pulp Stem Cells by Different Concentrations of beta-Glycerophosphate
Liu, Mingyue1; Sun, Yao2; Liu, Yang3; Yuan, Mengtong1; Zhang, Zhihui4; Hu, Weiping1
关键词Mepe Dsp Beta-glycerophosphate Dental Pulp Stem Cells Odontoblast
刊名MOLECULES
2012-02-01
DOI10.3390/molecules17021219
17期:2页:1219-1232
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Organic
研究领域[WOS]Chemistry
关键词[WOS]IN-VITRO ; BONE ; MINERALIZATION ; MATRIX ; EXPRESSION ; PROTEIN ; TISSUE ; GENE ; MEPE ; SIALOPHOSPHOPROTEIN
英文摘要

Dentinogenesis is a necessary prerequisite for dental tissue engineering. One of the steps for dentinogenesis is to obtain large quantities of highly purified odontoblasts. Therefore, we have undertaken an experiment applying different concentrations of beta-glycerophosphate (beta-GP) to induce the differentiation of dental pulp stem cells (DPSCs) in a long-term 28-day culture. In the meanwhile, we have studied the time-and maturation-dependent expression of matrix extracellular phosphoglycoprotein (MEPE) and that of the odontoblast-like marker-dentin sialoprotein (DSP), in order to investigate an optimized mineralized condition. Western blot results revealed that the expression of DSP became lower when accompanied by the increase of the beta-GP concentration, and there was also an influence on MEPE expression when different concentrations of beta-GP were applied. Meanwhile, the mineralized groups had an inhibitory function on the expression of MEPE as compared with the control group. Above all, all experimental groups successfully generated mineralized nodules by Alizarin Red S and the 5 mM beta-GP group formed more mineralized nodules quantitated using the CPC extraction method. In conclusion, there is a significant modulation of the beta-GP during the differentiation of the DPSCs. The degree of odontoblast differentiation is beta-glycerophosphate concentration dependent. A low concentration of beta-GP (5 mM) has been shown to be the optimal concentration for stimulating the maturation of the DPSCs. Moreover, MEPE accompanied with DSP clearly demonstrates the degree of the differentiation.

语种英语
WOS记录号WOS:000300717200007
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57415
专题北京大学口腔医学院
北京大学口腔医学院_第一门诊部
作者单位1.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
2.Harbin Med Coll, Dept Prosthodont, Affiliated Hosp 2, Harbin 150086, Heilongjiang, Peoples R China
3.Harbin Med Coll, Inst Hard Tissue Dev & Regenerat, Affiliated Hosp 2, Harbin 150086, Heilongjiang, Peoples R China
4.Mianyang Cent Hosp, Dept Stomatol, Mianyang 150086, Sichuan, Peoples R China
推荐引用方式
GB/T 7714
Liu, Mingyue,Sun, Yao,Liu, Yang,et al. Modulation of the Differentiation of Dental Pulp Stem Cells by Different Concentrations of beta-Glycerophosphate[J]. MOLECULES,2012,17(2):1219-1232.
APA Liu, Mingyue,Sun, Yao,Liu, Yang,Yuan, Mengtong,Zhang, Zhihui,&Hu, Weiping.(2012).Modulation of the Differentiation of Dental Pulp Stem Cells by Different Concentrations of beta-Glycerophosphate.MOLECULES,17(2),1219-1232.
MLA Liu, Mingyue,et al."Modulation of the Differentiation of Dental Pulp Stem Cells by Different Concentrations of beta-Glycerophosphate".MOLECULES 17.2(2012):1219-1232.
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