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学科主题: 基础医学
题名:
Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation
作者: Cui, Yi-Ran1; Hong, Chao2; Zhou, Ying-Lin1; Li, Yue3; Gao, Xiao-Ming2,3; Zhang, Xin-Xiang1
关键词: Fe3O4/Au magnetic nanoparticles ; (Fe3O4@Au MNPs) ; Oriented immobilization ; Antibody ; Protein A ; Cell separation
刊名: TALANTA
发表日期: 2011-09-15
DOI: 10.1016/j.talanta.2011.05.010
卷: 85, 期:3, 页:1246-1252
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: IRON-OXIDE NANOPARTICLES ; AFFINITY CAPTURE ; SHELL ; CORE ; PURIFICATION ; MICROSPHERES ; ORIENTATION ; PERFORMANCE ; PARTICLES ; BINDING
英文摘要:

Orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles were synthesized for cell separation. The Fe3O4@Au magnetic nanoparticles were synthesized by reducing HAuCl4 on the surfaces of Fe3O4 nanoparticles, which were further characterized in detail by TEM, XRD and UV-vis spectra. Anti-CD3 monoclonal antibody was orientedly bioconjugated to the surface of Fe3O4@Au nanoparticles through affinity binding between the Fc portion of the antibody and protein A that covalently immobilized on the nanoparticles. The oriented immobilization method was performed to compare its efficiency for cell separation with the non-oriented one, in which the antibody was directly immobilized onto the carboxylated nanoparticle surface. Results showed that the orientedly bioconjugated Fe3O4@Au MNPs successfully pulled down CD3(+) T cells from the whole splenocytes with high efficiency of up to 98.4%, showing a more effective cell-capture nanostructure than that obtained by non-oriented strategy. This developed strategy for the synthesis and oriented bioconjugation of Fe3O4@Au MNPs provides an efficient tool for cell separation, and may be further applied to various fields of bioanalytical chemistry for diagnosis, affinity extraction and biosensor. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30890142 ; 20975007 ; 20805002 ; 90713013
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000294092800005
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57416
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, BNLMS, Key Lab Biochem & Mol Engn, Minist Educ,Inst Analyt Chem,Coll Chem, Beijing 100871, Peoples R China
2.Soochow Univ, Inst Biol & Med Sci, Suzhou 215123, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100191, Peoples R China

Recommended Citation:
Cui, Yi-Ran,Hong, Chao,Zhou, Ying-Lin,et al. Synthesis of orientedly bioconjugated core/shell Fe3O4@Au magnetic nanoparticles for cell separation[J]. TALANTA,2011,85(3):1246-1252.
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