IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科
学科主题临床医学
Hexokinase II in CD133(+) and CD133(-) Hepatoma BEL-7402 Cells
Gong, Lei; Cui, Zhuqingqing; Yu, Xin; Wei, Yuhua; Peng, Jirun; Leng, Xisheng
关键词Hepatoma. Cd133 Hexokinase Ii Bel-7402 Therapeutic Target
刊名PATHOLOGY & ONCOLOGY RESEARCH
2012-04-01
DOI10.1007/s12253-011-9455-y
18期:2页:377-381
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Pathology
研究领域[WOS]Oncology ; Pathology
关键词[WOS]CANCER STEM-CELLS ; HEPATOCELLULAR-CARCINOMA CELLS ; ENERGY-METABOLISM ; PANCREATIC-CANCER ; PROGENITOR CELLS ; IDENTIFICATION ; EXPRESSION ; MITOCHONDRIA ; INHIBITOR ; APOPTOSIS
英文摘要

Hexokinase II is a key enzyme in the glycolytic pathway and CD133+ human hepatoma cells possess cancer stem cell-like properties. The expression and enzyme activity of hexokinase II in CD133+ and CD133- hepatoma cells were examined. CD133 on the surface of the hepatoma BEL-7402 cells was analyzed by flow cytometry and the cells were magnetically sorted into CD133+ and CD133- groups. CD133+ cells comprised 1.04% of the total BEL-7402 cell population. Reverse transcription-polymerase chain reaction (PCR) and quantitative real-time PCR were used to assay the expression of hexokinase II mRNA in these two groups. The level of mRNA in CD133- cells was 4.35 times greater than the level in CD133+ cells. 3,6-biphosphoglucose dehydrogenase-coupled colorimetric method and temperature-sensitive trials were applied to determine the enzyme activity of hexokinase II, which was 1.02 U/g protein in CD133+ cells and 2.47 U/g protein in CD133- cells. Hexokinase II was the major active hexokinase isoform in CD133+ cells, comprising 92.7% of the overall cellular hexokinase activity. The results indicate that hexokinase II is vitally meaningful for CD133+ hepatoma BEL-7402 cells. Hexokinase II represents a new therapeutic target for treating CD133+ hepatoma cells.

语种英语
WOS记录号WOS:000303538200033
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57419
Collection北京大学第二临床医学院_肝胆外科
北京大学精神卫生研究所_精神科
作者单位Peking Univ, Peoples Hosp, Dept Hepatobiliary Surg, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Gong, Lei,Cui, Zhuqingqing,Yu, Xin,et al. Hexokinase II in CD133(+) and CD133(-) Hepatoma BEL-7402 Cells[J]. PATHOLOGY & ONCOLOGY RESEARCH,2012,18(2):377-381.
APA Gong, Lei,Cui, Zhuqingqing,Yu, Xin,Wei, Yuhua,Peng, Jirun,&Leng, Xisheng.(2012).Hexokinase II in CD133(+) and CD133(-) Hepatoma BEL-7402 Cells.PATHOLOGY & ONCOLOGY RESEARCH,18(2),377-381.
MLA Gong, Lei,et al."Hexokinase II in CD133(+) and CD133(-) Hepatoma BEL-7402 Cells".PATHOLOGY & ONCOLOGY RESEARCH 18.2(2012):377-381.
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