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Effects of Apelin on RAW264.7 cells under both normal and hypoxic conditions
Yang, Fan; Bai, Yujing; Jiang, Yanrong
关键词Apelin Macrophage Hypoxia Inflammation Angiogenesis
刊名PEPTIDES
2015-07-01
DOI10.1016/j.peptides.2015.04.025
69页:133-143
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Pharmacology & Pharmacy
关键词[WOS]ENDOTHELIAL GROWTH-FACTOR ; MESSENGER-RNA EXPRESSION ; HUMAN COLORECTAL-CANCER ; TUMOR-NECROSIS-FACTOR ; INFLAMMATORY ANGIOGENESIS ; ALVEOLAR MACROPHAGES ; TRANSCRIPTION FACTOR ; GLUCOSE DEPRIVATION ; TISSUE INHIBITOR ; AORTIC-ANEURYSM
英文摘要

Macrophages are an important source of pro-inflammatory and pro-angiogenic factors, which can promote pathological processes involving inflammation and angiogenesis. This study investigated the effects of Apelin on macrophages under both normal and hypoxic conditions. Under normal culture conditions, Apelin down-regulated the mRNA expression levels of monocyte chemotactic protein 1 (MCP1), monocyte chemotactic protein 3 (MCP3), macrophage inflammatory protein 1 (MIP1 alpha, MIP1 beta), vascular endothelial growth factor A (VEGFA), Angiopoietin 2 (Ang2) and tumor necrosis factor alpha (TNF alpha). The supernatant concentrations of MCP1, MCP3, MIP1 alpha, MIP1 beta, macrophage inflammatory protein 2 (MIP2) and TNF alpha proteins were significantly decreased in the Apelin treated group. Hypoxia induced profound up-regulations of the angiogenic, chemokine, and inflammatory factors at both the mRNA and protein levels. Apelin suppressed the hypoxia-induced increases in MCP1, MCP3, MIP2, MIP1 beta and TNF alpha expression. The underlying mechanism of Apelin inhibit inflammation is regulating NF-kappa B/JNK signal pathway. Additionally, Apelin can protect macrophages from apoptosis and can enhance cell migration during hypoxia. And cleaved Caspase9/3 pathways were involved in Apelin inhibiting RAW264.7 apoptosis. In conclusion, we showed the effect of Apelin on RAW264.7 macrophage under normal and hypoxic condition, which could further influence the angiogenesis and inflammation process that promoted by macrophages. (C) 2015 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000355214300019
项目编号81271027 ; 94410
资助机构National Natural Science Fund from the National Natural Science Foundation of China ; EFSD/CDS/Lilly grant from European Foundation for the Study of Diabetes
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57426
专题北京大学第二临床医学院
北京大学第二临床医学院_眼科
作者单位Peking Univ, Peoples Hosp, Dept Ophthalmol,Minist Educ,Key Lab Vis Loss & Re, Beijing Key Lab Diag & Treatment Retinal & Choroi, Beijing 100044, Peoples R China
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GB/T 7714
Yang, Fan,Bai, Yujing,Jiang, Yanrong. Effects of Apelin on RAW264.7 cells under both normal and hypoxic conditions[J]. PEPTIDES,2015,69:133-143.
APA Yang, Fan,Bai, Yujing,&Jiang, Yanrong.(2015).Effects of Apelin on RAW264.7 cells under both normal and hypoxic conditions.PEPTIDES,69,133-143.
MLA Yang, Fan,et al."Effects of Apelin on RAW264.7 cells under both normal and hypoxic conditions".PEPTIDES 69(2015):133-143.
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