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学科主题: 药学
题名:
A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide
作者: Chen, CP; Zhang, LR; Peng, YF; Wang, XB; Wang, SQ; Zhang, LH
刊名: BIOCONJUGATE CHEMISTRY
发表日期: 2003-05-01
DOI: 10.1021/bc034004f
卷: 14, 期:3, 页:532-538
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: HUMAN TELOMERASE ACTIVITY ; CELLS ; INHIBITION ; MOLECULES ; MEMBRANE ; DELIVERY
英文摘要:

Peptide-oligonucleotide conjugates were synthesized using two strategies: a mimetic signal peptide-conjugated oligonucleotide was assembled stepwise on CPG by using 2,2-dimethyl-3-hydroxypropionic acid as a linker. To solve the precipitation problem in the coupling reaction caused by the electrostatic interaction of arginine-rich peptides and oligonucleotide, oligonucleotides were absorbed on an anion-exchange resin, and then the on-resin fragment was applied for the conjugation with arginine-rich peptide. The peptide-antisense oligonucleotides showed permeability to the cell membrane of HepG-2 cells.

语种: 英语
WOS记录号: WOS:000183071100005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57436
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Sch Chem & Mol Engn, Beijing 100871, Peoples R China
3.Beijing Inst Radiat Med, Beijing 100850, Peoples R China

Recommended Citation:
Chen, CP,Zhang, LR,Peng, YF,et al. A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide[J]. BIOCONJUGATE CHEMISTRY,2003,14(3):532-538.
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