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A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide
Chen, CP; Zhang, LR; Peng, YF; Wang, XB; Wang, SQ; Zhang, LH
刊名BIOCONJUGATE CHEMISTRY
2003-05-01
DOI10.1021/bc034004f
14期:3页:532-538
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary ; Chemistry, Organic
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HUMAN TELOMERASE ACTIVITY ; CELLS ; INHIBITION ; MOLECULES ; MEMBRANE ; DELIVERY
英文摘要

Peptide-oligonucleotide conjugates were synthesized using two strategies: a mimetic signal peptide-conjugated oligonucleotide was assembled stepwise on CPG by using 2,2-dimethyl-3-hydroxypropionic acid as a linker. To solve the precipitation problem in the coupling reaction caused by the electrostatic interaction of arginine-rich peptides and oligonucleotide, oligonucleotides were absorbed on an anion-exchange resin, and then the on-resin fragment was applied for the conjugation with arginine-rich peptide. The peptide-antisense oligonucleotides showed permeability to the cell membrane of HepG-2 cells.

语种英语
WOS记录号WOS:000183071100005
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57436
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Sch Chem & Mol Engn, Beijing 100871, Peoples R China
3.Beijing Inst Radiat Med, Beijing 100850, Peoples R China
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GB/T 7714
Chen, CP,Zhang, LR,Peng, YF,et al. A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide[J]. BIOCONJUGATE CHEMISTRY,2003,14(3):532-538.
APA Chen, CP,Zhang, LR,Peng, YF,Wang, XB,Wang, SQ,&Zhang, LH.(2003).A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide.BIOCONJUGATE CHEMISTRY,14(3),532-538.
MLA Chen, CP,et al."A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide".BIOCONJUGATE CHEMISTRY 14.3(2003):532-538.
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