北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 泌尿外科  > 期刊论文
学科主题: 临床医学
题名:
KCNJ1 inhibits tumor proliferation and metastasis and is a prognostic factor in clear cell renal cell carcinoma
作者: Guo, Zhongqiang1,2,3; Liu, Jin1,2,3; Zhang, Lian1,2,3; Su, Boxing1,2,3; Xing, Yunchao1,2,3; He, Qun1,2,3; Ci, Weimin4; Li, Xuesong1,2,3; Zhou, Liqun1,2,3
关键词: KCNJ1 ; Clear cell renal cell carcinoma ; Prognostic factor ; Proliferation ; Invasion ; Apoptosis
刊名: TUMOR BIOLOGY
发表日期: 2015-02-01
DOI: 10.1007/s13277-014-2746-7
卷: 36, 期:2, 页:1251-1259
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: SYNDROME TYPE-II ; SURFACE EXPRESSION ; ROMK ; CHANNEL ; CANCER ; CALCIUM ; KINASE ; PHOSPHORYLATION ; MUTATIONS ; MAGNESIUM
英文摘要:

Potassium inwardly rectifying channel, subfamily J, member 1 (KCNJ1), as an ATP-dependent potassium channel, plays an essential role in potassium balance. KCNJ1 variation is associated with multiple diseases, such as antenatal Bartter syndrome and diabetes. However, the role of KCNJ1 in clear cell renal cell carcinoma (ccRCC) is still unknown. Here, we studied the expression and function of KCNJ1 in ccRCC. The expression of KCNJ1 was evaluated in ccRCC tissues and cell lines by quantitative real-time PCR (qRT-PCR), Western blot, and immunohistochemistry analysis. The relationship between KCNJ1 expression and clinicopathological characteristics was analyzed. p3xFLAG-CMV-14 vector containing KCNJ1 was constructed and used for transfecting ccRCC cell lines 786-O and Caki-2. The effects of KCNJ1 on cell proliferation, invasion, and apoptosis were detected in ccRCC cell lines using cell proliferation assay, transwell assay, and flow cytometry, respectively. We found that KCNJ1 was low-expressed in ccRCC tissues samples and cell lines, and its expression level was significantly associated with tumor pathology grade (P = 0.002) and clinical stage (P = 0.023). Furthermore, the KCNJ1 expression was a prognostic factor of ccRCC patient′s survival (P = 0.033). The re-expression of KCNJ1 in 786-O and Caki-2 significantly inhibited cancer cell growth and invasion and promoted cancer cell apoptosis. Moreover, knockdown of KCNJ1 in HK-2 cells promoted cell proliferation. Collectively, these data highlight that KCNJ1, low-expressed in ccRCC and associated with poor prognosis, plays an important role in ccRCC cell growth and metastasis.

语种: 英语
所属项目编号: 81372746
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000350478200093
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57440
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Natl Urol Canc Ctr, Beijing 100034, Peoples R China
4.Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China

Recommended Citation:
Guo, Zhongqiang,Liu, Jin,Zhang, Lian,et al. KCNJ1 inhibits tumor proliferation and metastasis and is a prognostic factor in clear cell renal cell carcinoma[J]. TUMOR BIOLOGY,2015,36(2):1251-1259.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Guo, Zhongqiang]'s Articles
[Liu, Jin]'s Articles
[Zhang, Lian]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Guo, Zhongqiang]‘s Articles
[Liu, Jin]‘s Articles
[Zhang, Lian]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace