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学科主题临床医学
KCNJ1 inhibits tumor proliferation and metastasis and is a prognostic factor in clear cell renal cell carcinoma
Guo, Zhongqiang1,2,3; Liu, Jin1,2,3; Zhang, Lian1,2,3; Su, Boxing1,2,3; Xing, Yunchao1,2,3; He, Qun1,2,3; Ci, Weimin4; Li, Xuesong1,2,3; Zhou, Liqun1,2,3
关键词Kcnj1 Clear Cell Renal Cell Carcinoma Prognostic Factor Proliferation Invasion Apoptosis
刊名TUMOR BIOLOGY
2015-02-01
DOI10.1007/s13277-014-2746-7
36期:2页:1251-1259
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]SYNDROME TYPE-II ; SURFACE EXPRESSION ; ROMK ; CHANNEL ; CANCER ; CALCIUM ; KINASE ; PHOSPHORYLATION ; MUTATIONS ; MAGNESIUM
英文摘要

Potassium inwardly rectifying channel, subfamily J, member 1 (KCNJ1), as an ATP-dependent potassium channel, plays an essential role in potassium balance. KCNJ1 variation is associated with multiple diseases, such as antenatal Bartter syndrome and diabetes. However, the role of KCNJ1 in clear cell renal cell carcinoma (ccRCC) is still unknown. Here, we studied the expression and function of KCNJ1 in ccRCC. The expression of KCNJ1 was evaluated in ccRCC tissues and cell lines by quantitative real-time PCR (qRT-PCR), Western blot, and immunohistochemistry analysis. The relationship between KCNJ1 expression and clinicopathological characteristics was analyzed. p3xFLAG-CMV-14 vector containing KCNJ1 was constructed and used for transfecting ccRCC cell lines 786-O and Caki-2. The effects of KCNJ1 on cell proliferation, invasion, and apoptosis were detected in ccRCC cell lines using cell proliferation assay, transwell assay, and flow cytometry, respectively. We found that KCNJ1 was low-expressed in ccRCC tissues samples and cell lines, and its expression level was significantly associated with tumor pathology grade (P = 0.002) and clinical stage (P = 0.023). Furthermore, the KCNJ1 expression was a prognostic factor of ccRCC patient′s survival (P = 0.033). The re-expression of KCNJ1 in 786-O and Caki-2 significantly inhibited cancer cell growth and invasion and promoted cancer cell apoptosis. Moreover, knockdown of KCNJ1 in HK-2 cells promoted cell proliferation. Collectively, these data highlight that KCNJ1, low-expressed in ccRCC and associated with poor prognosis, plays an important role in ccRCC cell growth and metastasis.

语种英语
WOS记录号WOS:000350478200093
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57440
专题北京大学第一临床医学院_泌尿外科
北京大学第二临床医学院_放射科
作者单位1.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
2.Peking Univ, Inst Urol, Beijing 100034, Peoples R China
3.Natl Urol Canc Ctr, Beijing 100034, Peoples R China
4.Chinese Acad Sci, Beijing Inst Genom, Beijing 100101, Peoples R China
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GB/T 7714
Guo, Zhongqiang,Liu, Jin,Zhang, Lian,et al. KCNJ1 inhibits tumor proliferation and metastasis and is a prognostic factor in clear cell renal cell carcinoma[J]. TUMOR BIOLOGY,2015,36(2):1251-1259.
APA Guo, Zhongqiang.,Liu, Jin.,Zhang, Lian.,Su, Boxing.,Xing, Yunchao.,...&Zhou, Liqun.(2015).KCNJ1 inhibits tumor proliferation and metastasis and is a prognostic factor in clear cell renal cell carcinoma.TUMOR BIOLOGY,36(2),1251-1259.
MLA Guo, Zhongqiang,et al."KCNJ1 inhibits tumor proliferation and metastasis and is a prognostic factor in clear cell renal cell carcinoma".TUMOR BIOLOGY 36.2(2015):1251-1259.
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