|Expression of LAPTM4B in Gallbladder Carcinoma Cells: The Role in Invasive Potential|
|Zhou, Li1; He, Xiao-Dong1; Yu, Jian-Chun1; Zhou, Rou-Li2; Xiong, Fu-Xia2; Qu, Qiang1; Rui, Jing-An1|
|关键词||Laptm4b Expression Migration Invasion Gbc-sd|
|WOS标题词||Science & Technology|
|类目[WOS]||Gastroenterology & Hepatology ; Surgery|
|研究领域[WOS]||Gastroenterology & Hepatology ; Surgery|
|关键词[WOS]||HEPATOCYTE GROWTH-FACTOR ; HEPATOCELLULAR-CARCINOMA ; GENE-EXPRESSION ; IN-VITRO ; CANCER ; SURVIVAL ; OVEREXPRESSION ; CARCINOGENESIS ; PROGRESSION ; INHIBITION|
Background/Aims: It was previously established that LAPTM4B-35 highly expressed in gallbladder carcinoma and being of clinicopathological and prognostic significances. However, expression of LAPTM4B gene in gallbladder carcinoma (GBC-SD), a gallbladder carcinoma cell line, and its role in invasive potential remain unclear.
Methodology: Expression of LAPTM4B in GBC-SD cells was first detected. Plasmids, pcDNA3-AE (containing complete open reading frame of LAPTM4B) and Mock (pcDNA3), were transiently transfected into GBC-SD cells. Invasive phenotypes (migration and invasion) and relative molecules were then shown by transwell assay, crossing river test and Western blot analysis.
Results: Immunocytochemical staining revealed that LAPTM4B-35 positively expressed in cytoplasm of GBC-SD cells. But LAPTM4B-35 expression was obviously weaker in GBC-SD cells than that in BEL-7402 cells (positive control). Besides, cells transfected with pcDNA3-AE presented shorter crossing river time, less migrated and invaded cell numbers, compared with cells transfected with the Mock plasmid and parent cells. Finally, increased expressions of active uPA, MMP-9, pro MMP-2 and active MMP-2 were also observed in cells transfected with pcDNA3-AE.
Conclusions: Our data suggested that LAPTM4B expressed in GBC-SD cells at a relatively low level. Forced overexpression of LAPTM4B increased invasive potential of GBC-SD cells, through modulating molecules associated with degradation of extracellular matrix.
|作者单位||1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Peking Union Med Coll, Dept Gen Surg, Beijing 100730, Peoples R China|
2.Peking Univ, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China
|Zhou, Li,He, Xiao-Dong,Yu, Jian-Chun,et al. Expression of LAPTM4B in Gallbladder Carcinoma Cells: The Role in Invasive Potential[J]. HEPATO-GASTROENTEROLOGY,2010,57(98):207-211.|
|APA||Zhou, Li.,He, Xiao-Dong.,Yu, Jian-Chun.,Zhou, Rou-Li.,Xiong, Fu-Xia.,...&Rui, Jing-An.(2010).Expression of LAPTM4B in Gallbladder Carcinoma Cells: The Role in Invasive Potential.HEPATO-GASTROENTEROLOGY,57(98),207-211.|
|MLA||Zhou, Li,et al."Expression of LAPTM4B in Gallbladder Carcinoma Cells: The Role in Invasive Potential".HEPATO-GASTROENTEROLOGY 57.98(2010):207-211.|