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学科主题: 基础医学
题名:
Expression of LAPTM4B in Gallbladder Carcinoma Cells: The Role in Invasive Potential
作者: Zhou, Li1; He, Xiao-Dong1; Yu, Jian-Chun1; Zhou, Rou-Li2; Xiong, Fu-Xia2; Qu, Qiang1; Rui, Jing-An1
关键词: LAPTM4B ; Expression ; Migration ; Invasion ; GBC-SD
刊名: HEPATO-GASTROENTEROLOGY
发表日期: 2010-03-01
卷: 57, 期:98, 页:207-211
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Surgery
研究领域[WOS]: Gastroenterology & Hepatology ; Surgery
关键词[WOS]: HEPATOCYTE GROWTH-FACTOR ; HEPATOCELLULAR-CARCINOMA ; GENE-EXPRESSION ; IN-VITRO ; CANCER ; SURVIVAL ; OVEREXPRESSION ; CARCINOGENESIS ; PROGRESSION ; INHIBITION
英文摘要:

Background/Aims: It was previously established that LAPTM4B-35 highly expressed in gallbladder carcinoma and being of clinicopathological and prognostic significances. However, expression of LAPTM4B gene in gallbladder carcinoma (GBC-SD), a gallbladder carcinoma cell line, and its role in invasive potential remain unclear.

Methodology: Expression of LAPTM4B in GBC-SD cells was first detected. Plasmids, pcDNA3-AE (containing complete open reading frame of LAPTM4B) and Mock (pcDNA3), were transiently transfected into GBC-SD cells. Invasive phenotypes (migration and invasion) and relative molecules were then shown by transwell assay, crossing river test and Western blot analysis.

Results: Immunocytochemical staining revealed that LAPTM4B-35 positively expressed in cytoplasm of GBC-SD cells. But LAPTM4B-35 expression was obviously weaker in GBC-SD cells than that in BEL-7402 cells (positive control). Besides, cells transfected with pcDNA3-AE presented shorter crossing river time, less migrated and invaded cell numbers, compared with cells transfected with the Mock plasmid and parent cells. Finally, increased expressions of active uPA, MMP-9, pro MMP-2 and active MMP-2 were also observed in cells transfected with pcDNA3-AE.

Conclusions: Our data suggested that LAPTM4B expressed in GBC-SD cells at a relatively low level. Forced overexpression of LAPTM4B increased invasive potential of GBC-SD cells, through modulating molecules associated with degradation of extracellular matrix.

语种: 英语
所属项目编号: HK100230446
项目资助者: grant for the municipal key discipline of Beijing, China
WOS记录号: WOS:000278938200004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57443
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Peking Union Med Coll, Dept Gen Surg, Beijing 100730, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China

Recommended Citation:
Zhou, Li,He, Xiao-Dong,Yu, Jian-Chun,et al. Expression of LAPTM4B in Gallbladder Carcinoma Cells: The Role in Invasive Potential[J]. HEPATO-GASTROENTEROLOGY,2010,57(98):207-211.
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