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Sarcomeric-alpha-actinin defective in vinculin-binding causes Z-line expansion and nemaline-like body formation in cultured chick myotubes
Zhang, Zhi-Qian1; Bish, Lawrence T.2; Holtzer, Howard3; Sweeney, H. Lee2
关键词Sarcomeric-alpha-actinin Vinculin Yeast Two-hybridization Binding Site Z-line Width Nemaline Body
刊名EXPERIMENTAL CELL RESEARCH
2009-03-10
DOI10.1016/j.yexcr.2008.12.019
315期:5页:748-759
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Cell Biology
研究领域[WOS]Oncology ; Cell Biology
关键词[WOS]MUSCLE Z-BAND ; VERTEBRATE Z-DISCS ; SKELETAL-MUSCLE ; 3-DIMENSIONAL STRUCTURE ; CARDIAC MYOCYTES ; TITIN ; EXPRESSION ; PROTEINS ; REPEATS ; SITE
英文摘要

The Z-line in each striated muscle has a precisely defined width that corresponds to Muscle fiber type, and it can enlarge several fold in nemaline myopathy. To explore the mechanism(s) underlying Z-line width and structure maintenance, a series of sarcomeric-alpha-actinin mutants tagged with myc-epitope was transfected into Cultured chick myotubes. By double-staining transfected myotubes with myc and myofibrillar protein antibodies, we found that alpha-actinin Mutants with deletion of the region from the beginning of the fourth spectrin repeat to the start of the EF-hands resulted in expansion of Z-line width, often displayed a doublet staining pattern, and resulted in formation of nemaline-like bodies in older myotubes under fluorescence microscope. Yeast-two hybridization analysis demonstrated that this region was involved in vinculin binding, and for vinculin to bind alpha-actinin, residues 1-116 and 258-323 were required. Hence, we have defined a critical region of s-alpha-actinin that affects the width and integrity of the Z-line. This region is at least involved in the interaction with Vinculin. (C) 2008 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000263859200003
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57448
专题北京大学临床肿瘤学院
作者单位1.Univ Penn, Dept Cell & Dev Biol, Philadelphia, PA 19104 USA
2.Univ Penn, Dept Physiol, Philadelphia, PA 19104 USA
3.Peking Univ, Sch Oncol, Key Lab Carcinogenesis & Translat Res, Minis Educ,Clin Res Lab,Beijing Canc Hosp & Inst, Beijing 100142, Peoples R China
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Zhang, Zhi-Qian,Bish, Lawrence T.,Holtzer, Howard,et al. Sarcomeric-alpha-actinin defective in vinculin-binding causes Z-line expansion and nemaline-like body formation in cultured chick myotubes[J]. EXPERIMENTAL CELL RESEARCH,2009,315(5):748-759.
APA Zhang, Zhi-Qian,Bish, Lawrence T.,Holtzer, Howard,&Sweeney, H. Lee.(2009).Sarcomeric-alpha-actinin defective in vinculin-binding causes Z-line expansion and nemaline-like body formation in cultured chick myotubes.EXPERIMENTAL CELL RESEARCH,315(5),748-759.
MLA Zhang, Zhi-Qian,et al."Sarcomeric-alpha-actinin defective in vinculin-binding causes Z-line expansion and nemaline-like body formation in cultured chick myotubes".EXPERIMENTAL CELL RESEARCH 315.5(2009):748-759.
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