IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3
Peng, LR; Li, Y; Meng, L; Shou, CC
刊名HYBRIDOMA AND HYBRIDOMICS
2004-02-01
23期:1页:23-27
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]METASTASIS
英文摘要

PRL-3 is a newly identified nonclassical protein tyrosine phosphatase associated with colorectal cancer metastasis. To prepare specific monoclonal antibody (MAb) against PRL-3, cDNAs of PRL-1, - 2, and - 3 were cloned by RT-PCR and inserted into prokaryotic expression vector pGEX-4T1, respectively. The fusion proteins were expressed in Escherichia coli. Monoclonal antibody against PRL-3 was obtained with hybridoma technique and specific ELISA screening. Western blotting and immunohistochemistry assays showed that MAb 3B6 had specific binding ability with PRL-3 protein in eukaryotic cells. This MAb will be a helpful tool for the detection of PRL-3 protein in the tissues and serum of colorectal cancer and other cancer patients.

语种英语
WOS记录号WOS:000188971300004
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57453
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China
2.Ningxia Med Coll, Dept Biochem & Mol Biol, Ningxia, Peoples R China
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GB/T 7714
Peng, LR,Li, Y,Meng, L,et al. Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3[J]. HYBRIDOMA AND HYBRIDOMICS,2004,23(1):23-27.
APA Peng, LR,Li, Y,Meng, L,&Shou, CC.(2004).Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3.HYBRIDOMA AND HYBRIDOMICS,23(1),23-27.
MLA Peng, LR,et al."Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3".HYBRIDOMA AND HYBRIDOMICS 23.1(2004):23-27.
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