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学科主题: 临床医学
题名:
Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3
作者: Peng, LR; Li, Y; Meng, L; Shou, CC
刊名: HYBRIDOMA AND HYBRIDOMICS
发表日期: 2004-02-01
卷: 23, 期:1, 页:23-27
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: METASTASIS
英文摘要:

PRL-3 is a newly identified nonclassical protein tyrosine phosphatase associated with colorectal cancer metastasis. To prepare specific monoclonal antibody (MAb) against PRL-3, cDNAs of PRL-1, - 2, and - 3 were cloned by RT-PCR and inserted into prokaryotic expression vector pGEX-4T1, respectively. The fusion proteins were expressed in Escherichia coli. Monoclonal antibody against PRL-3 was obtained with hybridoma technique and specific ELISA screening. Western blotting and immunohistochemistry assays showed that MAb 3B6 had specific binding ability with PRL-3 protein in eukaryotic cells. This MAb will be a helpful tool for the detection of PRL-3 protein in the tissues and serum of colorectal cancer and other cancer patients.

语种: 英语
WOS记录号: WOS:000188971300004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57453
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Dept Biochem & Mol Biol, Beijing 100034, Peoples R China
2.Ningxia Med Coll, Dept Biochem & Mol Biol, Ningxia, Peoples R China

Recommended Citation:
Peng, LR,Li, Y,Meng, L,et al. Preparation and characterization of monoclonal antibody against protein tyrosine phosphatase PRL-3[J]. HYBRIDOMA AND HYBRIDOMICS,2004,23(1):23-27.
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