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学科主题基础医学
Doxycycline Enhances the Ras-MAPK Signaling and Proliferation of Mouse Thymic Epithelial Cells
Chen, Xun2; Xia, Sheng2; Li, Rong1; Liu, Hui3; Huang, Ying2; Qian, Xiaoping1; Xiao, Xueyuan3; Xu, Xun2; Lin, Xin2; Tian, Yuxiang2; Zong, Yangyong2; He, Dacheng3; Chen, Weifeng1; Zhang, Yu1; Shao, Qixiang2
关键词DOXCYCLINE MOUSE THYMIC EPITHELIAL CELLS CELL PROLIFERATION MAPK
刊名JOURNAL OF CELLULAR BIOCHEMISTRY
2009-06-01
DOI10.1002/jcb.22147
107期:3页:494-503
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]CHEMICALLY-MODIFIED TETRACYCLINES ; IMMUNE RECONSTITUTION SYNDROME ; THYMOCYTE DEVELOPMENT ; RHEUMATOID-ARTHRITIS ; CYCLE REGULATION ; CANCER CELLS ; LINE MTEC1 ; IN-VITRO ; INHIBITION ; APOPTOSIS
英文摘要

Depletion of T-cell-dependent immunity is a major consideration for patients suffering from infections of human immunodeficiency virus (HIV), those undergoing organ transplantation, and those receiving anti-cancer chemotherapy and/or radiotherapy. In general, T-cell regeneration occurs in the thymus through thymopoiesis. We have found that doxycycline (Dox), a tetracycline derivative, enhances the proliferation of mouse thymic epithelial cells, which are unique in their capacity to support positive selection and are essential throughout the development of thymocytes. Cell cycle analysis indicates that the increased cell proliferation is due to a shortened G(0)/G(1) phase. To reveal the underlying mechanisms, we examined the expression of an array of molecules that. regulate the cell cycle. The results show that in mouse thymic medullary-type epithelial cell line 1 (MTEC1) Dox leads to elevated levels of H-Ras, phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), cyclin E, cyclin dependent kinase 4/2 (CDK4/CDK2), E2F3, and c-myc. These data, and the observation that the proliferation-enhancing effect is largely abolished following treatment with an ERK inhibitor support an active role of the Ras-ERK/mitogen-activated protein kinase (MAPK) signaling pathway. In conclusion, the present study reveals a new activity or an old family of antibiotics. The in vivo effect of Dox on immune reconstitution warrants further exploration. J. Cell. Biochem. 107: 494-503, 2009. (C) 2009 Wiley-Liss, Inc.

语种英语
WOS记录号WOS:000266872300013
项目编号2006C13910101 ; 3033053904 ; 30671984 ; 2008-018-02
资助机构Chinese National 973 Program ; National Science Foundation of China ; Sci-tech Innovation Team of Jiangsu University
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57456
专题北京大学基础医学院_免疫学系
北京大学基础医学院
北京大学第三临床医学院_生殖医学中心
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100191, Peoples R China
2.Jiangsu Univ, Sch Med Sci & Lab Med, Dept Immunol, Zhenjiang 212013, Jiangsu, Peoples R China
3.Beijing Normal Univ, Inst Cell Biol, Beijing 100875, Peoples R China
推荐引用方式
GB/T 7714
Chen, Xun,Xia, Sheng,Li, Rong,et al. Doxycycline Enhances the Ras-MAPK Signaling and Proliferation of Mouse Thymic Epithelial Cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2009,107(3):494-503.
APA Chen, Xun.,Xia, Sheng.,Li, Rong.,Liu, Hui.,Huang, Ying.,...&Shao, Qixiang.(2009).Doxycycline Enhances the Ras-MAPK Signaling and Proliferation of Mouse Thymic Epithelial Cells.JOURNAL OF CELLULAR BIOCHEMISTRY,107(3),494-503.
MLA Chen, Xun,et al."Doxycycline Enhances the Ras-MAPK Signaling and Proliferation of Mouse Thymic Epithelial Cells".JOURNAL OF CELLULAR BIOCHEMISTRY 107.3(2009):494-503.
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