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学科主题临床医学
Homocysteine potentiates calcification of cultured rat aortic smooth muscle cells
Li, JX; Chai, SB; Tang, CS; Du, JB
关键词Homocysteine Calcification Vascular Smooth Muscle Cells Alkaline Phosphatase
刊名LIFE SCIENCES
2003-12-12
DOI10.1016/j.lfs.2003.06.028
74期:4页:451-461
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]PERIPHERAL VASCULAR-DISEASE ; ARTERIAL CALCIFICATION ; RISK-FACTORS ; MAP KINASE ; ATHEROSCLEROSIS ; PLASMA ; ASSOCIATION ; DIFFERENTIATION ; MINERALIZATION ; DEPOSITS
英文摘要

Aortic calcification was demonstrated in experimental animal models of hyperhomocysteinemia. Mild hyperhomocysteinemia was associated with aortic calcification, suggesting a relationship between homocysteine (HCY) and the pathogenesis of aortic calcification. In the present study, the effect of HCY on vascular calcification was examined in calcifying and non-calcifying vascular smooth muscle cells (VSMCs). Cell calcification was induced by incubation of VSMCs with [ glycerophosphate. Proliferation of VSMCs was studied by cell counting, H-3-thymidine (H-3-TdR) and H-3-leucine (H-3-Leu) incorporation. Ca-45 accumulation, cell calcium content, and alkaline phosphatase (ALP) activity were measured as indices of calcification. The results showed that the proliferation of calcifying VSMCs, which was indicated by cell counting, H-3-TdR and H-3-Leu incorporation in calcifying VSMCs, was enhanced as compared with that of non-calcifying VSMCs. HCY promoted increases in cell number, H-3-TdR and H-3-Leu incorporation in both calcifying and non-calcifying VSMCs, but with more prominent effect in calcifying VSMCs. The stimulating effects of HCY on the three parameters in calcifying VSMCs were antagonized by PD98059, a specific inhibitor of mitogen activated protein kinase kinase (MAPKK). The ALP activity, Ca-45 uptake, and calcium deposition in the calcifying VSMCs were greater than those in non-calcifying VSMCs. PD98059 had no effect on ALP activity, Ca-41 uptake, and calcium deposition in calcifying VSMCs. HCY caused marked increases in Ca-45 uptake and calcium deposition both in calcifying and non-calcifying VSMCs. HCY, however, enhanced ALP activity in the calcified VSMCs but not in the non-calcifying VSMCs. The non-calcifying VSMCs treated with HCY showed the same low ALP activity, as did the control VSMCs. In calcifying VSMCs, the HCY-induced increases in Ca-45 uptake, calcium deposition, and ALP activity were also attenuated by PD98059. The results demonstrated that HCY potentiated VSMC calcification probably through the mechanisms by which HCY promotes atherosclerosis. (C) 2003 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000186642400004
引用统计
被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57459
专题北京大学第一临床医学院_心血管内科
作者单位1.Peking Univ, Dept Physiol & Pathophysiol, Hlth Sci Ctr, Beijing 100083, Peoples R China
2.Peking Univ, Hosp 1, Inst Cardiovasc Res, Beijing 100034, Peoples R China
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GB/T 7714
Li, JX,Chai, SB,Tang, CS,et al. Homocysteine potentiates calcification of cultured rat aortic smooth muscle cells[J]. LIFE SCIENCES,2003,74(4):451-461.
APA Li, JX,Chai, SB,Tang, CS,&Du, JB.(2003).Homocysteine potentiates calcification of cultured rat aortic smooth muscle cells.LIFE SCIENCES,74(4),451-461.
MLA Li, JX,et al."Homocysteine potentiates calcification of cultured rat aortic smooth muscle cells".LIFE SCIENCES 74.4(2003):451-461.
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