IR@PKUHSC  > 北京大学第三临床医学院  > 肿瘤放疗科
学科主题临床医学
Inhibition of Signal Transducer and Activator of Transcription 3 and Cyclooxygenase-2 Is Involved in Radiosensitization of Cepharanthine in HeLa Cells
Fang, Zhen-hao; Li, Yan-jing; Chen, Zan; Wang, Jun-jie; Zhu, Li-hong
关键词Hela Cepharanthine Radiosensitization Stat3 Cox-2
刊名INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER
2013-05-01
DOI10.1097/IGC.0b013e31828a05fd
23期:4页:608-614
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Obstetrics & Gynecology
研究领域[WOS]Oncology ; Obstetrics & Gynecology
关键词[WOS]CERVICAL-CANCER ; ANTITUMOR-ACTIVITY ; KAPPA-B ; STAT3 ; RADIATION ; APOPTOSIS ; PATHWAY
英文摘要

Objectives: To investigate the radiosensitizing effects of cepharanthine (CEP) in the human cervical adenocarcinoma HeLa cell line and to examine the underlying mechanisms.

Materials/Methods: Survival of HeLa cells after treatment with or without ionizing radiation (IR) and CEP administration was investigated. MTT assays and apoptosis analysis were used to assess cytotoxicity. Nude mouse xenografts were established to evaluate the antitumor effects of CEP and IR in vivo. Expression of signal transducer and activator of transcription 3 (STAT3) and its downstream signaling molecules as well as cyclooxygenase-2 (COX-2) were examined by Western blot analysis.

Results: Clonogenic assays showed that treatment with CEP and IR resulted in significant radiosensitization. Cepharanthine and IR treatment achieved maximum cytotoxic effects on HeLa cells with regard to apoptosis induction. Cepharanthine efficiently decreased IR-induced STAT3 and COX-2 activation. The STAT3 target genes, including the antiapoptotic Bcl-2 and the cell cycle regulator c-Myc, were decreased concomitantly. In vivo administration of CEP (20 mg/kg every 2 days) combined with radiation in HeLa xenografts enhanced tumor growth delay and apoptosis (indicated by activated caspase-3 Western blot analysis), with reduced expression of STAT3, Bcl-2, c-Myc, and COX-2.

Conclusions: Cepharanthine was shown to induce radiation sensitization in HeLa cells in vitro and in vivo. The inhibitory effects of CEP on STAT3 signaling pathway and COX-2 help us to better understand the radiosensitization of CEP.

语种英语
WOS记录号WOS:000318071300006
Citation statistics
Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57462
Collection北京大学第三临床医学院_肿瘤放疗科
作者单位Peking Univ, Dept Radiat Oncol, Hosp 3, Beijing 100191, Peoples R China
Recommended Citation
GB/T 7714
Fang, Zhen-hao,Li, Yan-jing,Chen, Zan,et al. Inhibition of Signal Transducer and Activator of Transcription 3 and Cyclooxygenase-2 Is Involved in Radiosensitization of Cepharanthine in HeLa Cells[J]. INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,2013,23(4):608-614.
APA Fang, Zhen-hao,Li, Yan-jing,Chen, Zan,Wang, Jun-jie,&Zhu, Li-hong.(2013).Inhibition of Signal Transducer and Activator of Transcription 3 and Cyclooxygenase-2 Is Involved in Radiosensitization of Cepharanthine in HeLa Cells.INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER,23(4),608-614.
MLA Fang, Zhen-hao,et al."Inhibition of Signal Transducer and Activator of Transcription 3 and Cyclooxygenase-2 Is Involved in Radiosensitization of Cepharanthine in HeLa Cells".INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER 23.4(2013):608-614.
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