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学科主题: 临床医学
题名:
CMTM3 is frequently reduced in clear cell renal cell carcinoma and exhibits tumor suppressor activities
作者: Xie, J.; Yuan, Y.; Liu, Z.; Xiao, Y.; Zhang, X.; Qin, C.; Sheng, Z.; Xu, T.; Wang, X.
关键词: Clear cell renal cell carcinoma ; CMTM3 ; Tumor suppressor gene ; Transmembrane 4 superfamily ; Gene therapy
刊名: CLINICAL & TRANSLATIONAL ONCOLOGY
发表日期: 2014-04-01
DOI: 10.1007/s12094-013-1092-3
卷: 16, 期:4, 页:402-409
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: EXPRESSION ; CANCER ; APOPTOSIS ; METHYLATION ; RECEPTOR ; CLONING ; MARVEL ; GENES
英文摘要:

CKLF-like MARVEL transmembrane domain containing member 3 (CMTM3) is silenced in many kinds of cancers and inhibits tumor cells growth. We investigated the expression and role of CMTM3 in clear cell renal cell carcinoma (ccRCC).

The expression of CMTM3 was detected in ccRCC tissue microarray, specimens, and cell lines by immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively. After transfected with CMTM3 plasmid or vector, the proliferation and migration of ccRCC 786-0 cells were determined by MTT assay and transwell assay, respectively. Furthermore, the anchorage-independent growth of transfected cells was assessed using soft agar colony formation assay.

CMTM3 was down-regulated in 84 % (63/75) of ccRCC tissues and its expression had no correlation with the gender, age, clinical staging and histologic grade. CMTM3 protein was undetectable by western blot in most detected ccRCC specimens and two RCC cell lines (786-0 and ACHN). qRT-PCR analysis showed that CMTM3 mRNA was dramatically down-regulated in 40 ccRCC cancer tissues as compared with the paired adjacent normal ones. Restoration of CMTM3 significantly suppressed the anchorage-independent growth, proliferation and migration of 786-0 cells.

These results indicate that CMTM3 is significantly down-regulated in ccRCC and exerts remarkable tumor-suppressive functions in 786-0 cells. Reduction of CMTM3 expression may contribute to the pathogenesis of ccRCC and CMTM3 may be a potentially target for therapeutic strategy.

语种: 英语
所属项目编号: 31171341
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000333257200009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57473
Appears in Collections:北京大学第二临床医学院_泌尿外科_期刊论文

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作者单位: Peking Univ, Dept Urol, Peoples Hosp, Beijing 100044, Peoples R China

Recommended Citation:
Xie, J.,Yuan, Y.,Liu, Z.,et al. CMTM3 is frequently reduced in clear cell renal cell carcinoma and exhibits tumor suppressor activities[J]. CLINICAL & TRANSLATIONAL ONCOLOGY,2014,16(4):402-409.
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