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学科主题临床医学
CMTM3 is frequently reduced in clear cell renal cell carcinoma and exhibits tumor suppressor activities
Xie, J.; Yuan, Y.; Liu, Z.; Xiao, Y.; Zhang, X.; Qin, C.; Sheng, Z.; Xu, T.; Wang, X.
关键词Clear Cell Renal Cell Carcinoma Cmtm3 Tumor Suppressor Gene Transmembrane 4 Superfamily Gene Therapy
刊名CLINICAL & TRANSLATIONAL ONCOLOGY
2014-04-01
DOI10.1007/s12094-013-1092-3
16期:4页:402-409
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]EXPRESSION ; CANCER ; APOPTOSIS ; METHYLATION ; RECEPTOR ; CLONING ; MARVEL ; GENES
英文摘要

CKLF-like MARVEL transmembrane domain containing member 3 (CMTM3) is silenced in many kinds of cancers and inhibits tumor cells growth. We investigated the expression and role of CMTM3 in clear cell renal cell carcinoma (ccRCC).

The expression of CMTM3 was detected in ccRCC tissue microarray, specimens, and cell lines by immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR) and western blot, respectively. After transfected with CMTM3 plasmid or vector, the proliferation and migration of ccRCC 786-0 cells were determined by MTT assay and transwell assay, respectively. Furthermore, the anchorage-independent growth of transfected cells was assessed using soft agar colony formation assay.

CMTM3 was down-regulated in 84 % (63/75) of ccRCC tissues and its expression had no correlation with the gender, age, clinical staging and histologic grade. CMTM3 protein was undetectable by western blot in most detected ccRCC specimens and two RCC cell lines (786-0 and ACHN). qRT-PCR analysis showed that CMTM3 mRNA was dramatically down-regulated in 40 ccRCC cancer tissues as compared with the paired adjacent normal ones. Restoration of CMTM3 significantly suppressed the anchorage-independent growth, proliferation and migration of 786-0 cells.

These results indicate that CMTM3 is significantly down-regulated in ccRCC and exerts remarkable tumor-suppressive functions in 786-0 cells. Reduction of CMTM3 expression may contribute to the pathogenesis of ccRCC and CMTM3 may be a potentially target for therapeutic strategy.

语种英语
WOS记录号WOS:000333257200009
项目编号31171341
资助机构National Natural Science Foundation of China
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57473
专题北京大学第二临床医学院_泌尿外科
北京大学基础医学院
北京大学第三临床医学院_麻醉科
作者单位Peking Univ, Dept Urol, Peoples Hosp, Beijing 100044, Peoples R China
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Xie, J.,Yuan, Y.,Liu, Z.,et al. CMTM3 is frequently reduced in clear cell renal cell carcinoma and exhibits tumor suppressor activities[J]. CLINICAL & TRANSLATIONAL ONCOLOGY,2014,16(4):402-409.
APA Xie, J..,Yuan, Y..,Liu, Z..,Xiao, Y..,Zhang, X..,...&Wang, X..(2014).CMTM3 is frequently reduced in clear cell renal cell carcinoma and exhibits tumor suppressor activities.CLINICAL & TRANSLATIONAL ONCOLOGY,16(4),402-409.
MLA Xie, J.,et al."CMTM3 is frequently reduced in clear cell renal cell carcinoma and exhibits tumor suppressor activities".CLINICAL & TRANSLATIONAL ONCOLOGY 16.4(2014):402-409.
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