IR@PKUHSC  > 北京大学第一临床医学院  > 胸外科
学科主题临床医学
miR-130a regulates macrophage polarization and is associated with non-small cell lung cancer
Lin, Lin1; Lin, Haibo2; Wang, Lin1; Wang, Bin1; Hao, Xuezhi1; Shi, Yuankai1
关键词Non-small Cell Lung Cancer Mir-130a Macrophages Polarization
刊名ONCOLOGY REPORTS
2015-12-01
DOI10.3892/or.2015.4301
34期:6页:3088-3096
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TUMOR-ASSOCIATED MACROPHAGES ; PPAR-GAMMA ACTIVATION ; ANTICANCER THERAPIES ; HUMAN MONOCYTES ; GENE SIGNATURE ; BREAST-CANCER ; MICRORNAS ; RESPONSES ; TARGETS ; DIFFERENTIATION
英文摘要

Lung cancer is the most common cancer as well as the leading cause of cancer-related mortalities worldwide. Macrophages are the most abundant immune cells in primary and metastatic tumors, and contribute to tumor initiation, progression and metastasis. Macrophages have been shown to demonstrate a high level of plasticity, with the ability to undergo dynamic transition between M1 and M2 polarized phenotypes. In the present study, we investigated a pivotal role of miR-130a in macrophage polarization and whether it was associated with poor prognosis in non-small cell lung cancer (NSCLC), using RT-qPCR and western blot analyses. The in vitro experiments showed that miRNA-130a was expressed at a higher level in M1 compared to M2 macrophages. The enforced expression of miR-130a in macrophages resulted in a significantly increased production of proinflammatory cytokines, whereas deletion of miR-130a impaired the M2-associated gene expression and led to an M1-biased response. Mechanistically, the bioinformatics analysis revealed that proliferator-activated receptor gamma (PPAR gamma) is a potential target of miR-130a. Additionally, the luciferase assay confirmed that PPAR gamma translation was suppressed by miR-130a through the interaction with the 3′UTR of PPAR gamma mRNA. A subsequent analysis revaled that the induction of miR-130a suppressed PPAR gamma protein expression. In NSCLC patients, the results showed that miR-130a downregulation exhibited clinical relevance as it was correlated with poor prognosis and increased tumor stage and metastasis. In addition, miR-130a was inversely correlated with the macrophage marker, CD163, and target gene, PPAR gamma. Taken together, the results established miR-130a as a molecular switch during macrophage development and as a potential target for the treatment of NSCLC.

语种英语
WOS记录号WOS:000364617000032
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57488
专题北京大学第一临床医学院_胸外科
作者单位1.Chinese Acad Med Sci, Peking Union Med Coll, Canc Inst Hosp, Dept Med Oncol, Beijing 100021, Peoples R China
2.Peking Univ, Hosp 1, Dept Thorac Surg, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Lin, Lin,Lin, Haibo,Wang, Lin,et al. miR-130a regulates macrophage polarization and is associated with non-small cell lung cancer[J]. ONCOLOGY REPORTS,2015,34(6):3088-3096.
APA Lin, Lin,Lin, Haibo,Wang, Lin,Wang, Bin,Hao, Xuezhi,&Shi, Yuankai.(2015).miR-130a regulates macrophage polarization and is associated with non-small cell lung cancer.ONCOLOGY REPORTS,34(6),3088-3096.
MLA Lin, Lin,et al."miR-130a regulates macrophage polarization and is associated with non-small cell lung cancer".ONCOLOGY REPORTS 34.6(2015):3088-3096.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lin, Lin]的文章
[Lin, Haibo]的文章
[Wang, Lin]的文章
百度学术
百度学术中相似的文章
[Lin, Lin]的文章
[Lin, Haibo]的文章
[Wang, Lin]的文章
必应学术
必应学术中相似的文章
[Lin, Lin]的文章
[Lin, Haibo]的文章
[Wang, Lin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。