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学科主题: 临床医学
题名:
A New PCR-Based Mass Spectrometry System for High-Risk HPV, Part I Methods
作者: Yi, Xin1,2,7; Li, Jingjing1,4; Yu, Shuang1,5; Zhang, Aipeng3; Xu, Jiajia1,6; Yi, Ji1; Zou, Jing1; Nie, Xifang1; Huang, Jian1; Wang, Jian1
关键词: Human papillomavirus ; HPV ; Matrix-assisted laser desorption/ionization time-of-flight ; MALDI-TOF mass spectrometry ; Genotyping
刊名: AMERICAN JOURNAL OF CLINICAL PATHOLOGY
发表日期: 2011-12-01
DOI: 10.1309/AJCPWTZDT0Q7DOVI
卷: 136, 期:6, 页:913-919
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pathology
研究领域[WOS]: Pathology
关键词[WOS]: HUMAN-PAPILLOMAVIRUS DNA ; POLYMERASE-CHAIN-REACTION ; INTRAEPITHELIAL NEOPLASIA ; CERVICAL-CANCER ; WOMEN ; IDENTIFICATION ; AMPLIFICATION ; SEQUENCE ; SMEARS ; TESTS
英文摘要:

Infection with high-risk (HR) human papillomaviruses (HPVs) has been confirmed as the necessary cause of cervical cancer. There are many studies that have established and confirmed the relationship of specific HPV types and the risk of invasive cervical cancer. We have developed a novel genotyping method for detecting 14 HR-HP V genotypes simultaneously with MassARRAY (Sequenom, San Diego, CA) technique based on the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). All 14 HPVs showed high specificities and high sensitivities in the plasmid test,. lower detection limits for each genotype were from 10 to 100 copies. Furthermore, the MS system has high-throughput capacities, capable of processing, with type-specific output, 4,500 samples in 24 hours. The MS HPV assay is a sensitive and useful tool for HPV genotyping. It has the potential to be suitable for large-scale epidemiologic studies and routine diagnostic clinical applications owing to its high-throughput capacity, high sensitivity, and low cost per case.

语种: 英语
所属项目编号: 30725008 ; 30890032 ; 30811130531 ; 2006AA02A302 ; 2009AA022707 ; ZD200806180054A ; JC200903190768A ; CXB200903110066A ; JC200903190767A
项目资助者: Shenzhen Development and Reform Commission ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; Shenzhen Bureau of Science Technology &amp ; Information, Shenzhen, China ; International Innovation Team on the Cancer Genome Research of GuangDong province
WOS记录号: WOS:000297274500012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57493
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Beijing Genom Inst, Shenzhen 518083, Peoples R China
2.Beijing Inst Genom, Shenzhen, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
4.Chinese Peoples Liberat Army, Gen Hosp, Dept Obstet & Gynecol, Beijing, Peoples R China
5.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Genet Lab,Minist Educ,Key Lab Carcinogenesis & Tr, Beijing 100871, Peoples R China
6.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Endocrinol, Guangzhou 510275, Guangdong, Peoples R China
7.Shenzhen Univ, Sch Med, Genome Res Inst, Shenzhen, Peoples R China

Recommended Citation:
Yi, Xin,Li, Jingjing,Yu, Shuang,et al. A New PCR-Based Mass Spectrometry System for High-Risk HPV, Part I Methods[J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY,2011,136(6):913-919.
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