IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
A New PCR-Based Mass Spectrometry System for High-Risk HPV, Part I Methods
Yi, Xin1,2,7; Li, Jingjing1,4; Yu, Shuang1,5; Zhang, Aipeng3; Xu, Jiajia1,6; Yi, Ji1; Zou, Jing1; Nie, Xifang1; Huang, Jian1; Wang, Jian1
关键词Human Papillomavirus Hpv Matrix-assisted Laser Desorption/ionization Time-of-flight Maldi-tof Mass Spectrometry Genotyping
刊名AMERICAN JOURNAL OF CLINICAL PATHOLOGY
2011-12-01
DOI10.1309/AJCPWTZDT0Q7DOVI
136期:6页:913-919
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pathology
研究领域[WOS]Pathology
关键词[WOS]HUMAN-PAPILLOMAVIRUS DNA ; POLYMERASE-CHAIN-REACTION ; INTRAEPITHELIAL NEOPLASIA ; CERVICAL-CANCER ; WOMEN ; IDENTIFICATION ; AMPLIFICATION ; SEQUENCE ; SMEARS ; TESTS
英文摘要

Infection with high-risk (HR) human papillomaviruses (HPVs) has been confirmed as the necessary cause of cervical cancer. There are many studies that have established and confirmed the relationship of specific HPV types and the risk of invasive cervical cancer. We have developed a novel genotyping method for detecting 14 HR-HP V genotypes simultaneously with MassARRAY (Sequenom, San Diego, CA) technique based on the matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). All 14 HPVs showed high specificities and high sensitivities in the plasmid test,. lower detection limits for each genotype were from 10 to 100 copies. Furthermore, the MS system has high-throughput capacities, capable of processing, with type-specific output, 4,500 samples in 24 hours. The MS HPV assay is a sensitive and useful tool for HPV genotyping. It has the potential to be suitable for large-scale epidemiologic studies and routine diagnostic clinical applications owing to its high-throughput capacity, high sensitivity, and low cost per case.

语种英语
WOS记录号WOS:000297274500012
项目编号30725008 ; 30890032 ; 30811130531 ; 2006AA02A302 ; 2009AA022707 ; ZD200806180054A ; JC200903190768A ; CXB200903110066A ; JC200903190767A
资助机构Shenzhen Development and Reform Commission ; National Natural Science Foundation of China ; National High Technology Research and Development Program of China (863 Program) ; Shenzhen Bureau of Science Technology &amp ; Information, Shenzhen, China ; International Innovation Team on the Cancer Genome Research of GuangDong province
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57493
专题北京大学临床肿瘤学院
作者单位1.Beijing Genom Inst, Shenzhen 518083, Peoples R China
2.Beijing Inst Genom, Shenzhen, Peoples R China
3.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
4.Chinese Peoples Liberat Army, Gen Hosp, Dept Obstet & Gynecol, Beijing, Peoples R China
5.Peking Univ, Sch Oncol, Beijing Canc Hosp & Inst, Genet Lab,Minist Educ,Key Lab Carcinogenesis & Tr, Beijing 100871, Peoples R China
6.Sun Yat Sen Univ, Affiliated Hosp 1, Dept Endocrinol, Guangzhou 510275, Guangdong, Peoples R China
7.Shenzhen Univ, Sch Med, Genome Res Inst, Shenzhen, Peoples R China
推荐引用方式
GB/T 7714
Yi, Xin,Li, Jingjing,Yu, Shuang,et al. A New PCR-Based Mass Spectrometry System for High-Risk HPV, Part I Methods[J]. AMERICAN JOURNAL OF CLINICAL PATHOLOGY,2011,136(6):913-919.
APA Yi, Xin.,Li, Jingjing.,Yu, Shuang.,Zhang, Aipeng.,Xu, Jiajia.,...&Wang, Jian.(2011).A New PCR-Based Mass Spectrometry System for High-Risk HPV, Part I Methods.AMERICAN JOURNAL OF CLINICAL PATHOLOGY,136(6),913-919.
MLA Yi, Xin,et al."A New PCR-Based Mass Spectrometry System for High-Risk HPV, Part I Methods".AMERICAN JOURNAL OF CLINICAL PATHOLOGY 136.6(2011):913-919.
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