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学科主题: 基础医学
题名:
Separation and analysis of the soluble trimer of A beta(1-40) and its effects on the rise in intracellular calcium
作者: Zheng Xiaohui; Wang Lijun; Zhang Lan; Hong Yuankai; Huang Lixin; Sha Yinlin
关键词: soluble A beta(1-40) trimer ; size-exclusion chromatography (SEC) ; intracellular calcium ; native PAGE
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2006-04-01
DOI: 10.1007/s11434-006-0830-0
卷: 51, 期:7, 页:830-838
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: AMYLOID BETA-PROTEIN ; ALZHEIMERS-DISEASE ; SECONDARY STRUCTURE ; PEPTIDES ; MECHANISM ; OLIGOMERS ; PROTOFIBRILS ; PATHOGENESIS ; AGGREGATION ; MODEL
英文摘要:

The oligomers of A beta(1-40) peptide in PBS buffer solution were analyzed by SEC and native PAGE, and the trimer of A beta(1.40) was also isolated by SEC. In addition, the effects of the soluble A beta(1.40) trimer on intracellular free calcium (Ca2+) balance of hippocampal neurons of postnatal rats were investigated by fluorescence microscopy. The experimental results indicated that A beta(1.40) peptide existed in the form of low molecular weight oligomers in 0.231 mmol/L fresh A beta(1.40) solution (20 mmol/L sodium phosphate buffer, pH 7.4, 0.02% sodium azide) within 24 h and the soluble trimer was the most abundant species. Both the trimeric and the fibrillar A beta(1-40) were able to increase the intracellular Ca2+ concentration, but the A beta(1-40) trimer caused a gradual rise and the potential was also stronger than that of the fibrils at the same concentration. In addition there were different response modes for trimeric and fibrillar A beta(1-40), meaning that there are different mechanisms of increase in intracellular Ca2+ caused by A beta(1-40).

语种: 英语
WOS记录号: WOS:000237493500011
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57499
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: Peking Univ, Sch Basic Med Sci, Dept Biophys, Single Mol & Nanobiol Lab, Beijing 100083, Peoples R China

Recommended Citation:
Zheng Xiaohui,Wang Lijun,Zhang Lan,et al. Separation and analysis of the soluble trimer of A beta(1-40) and its effects on the rise in intracellular calcium[J]. CHINESE SCIENCE BULLETIN,2006,51(7):830-838.
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