北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 生物物理学系  > 期刊论文
学科主题: 基础医学
题名:
Separation and analysis of the soluble trimer of A beta(1-40) and its effects on the rise in intracellular calcium
作者: Zheng Xiaohui; Wang Lijun; Zhang Lan; Hong Yuankai; Huang Lixin; Sha Yinlin
关键词: soluble A beta(1-40) trimer ; size-exclusion chromatography (SEC) ; intracellular calcium ; native PAGE
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2006-04-01
DOI: 10.1007/s11434-006-0830-0
卷: 51, 期:7, 页:830-838
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: AMYLOID BETA-PROTEIN ; ALZHEIMERS-DISEASE ; SECONDARY STRUCTURE ; PEPTIDES ; MECHANISM ; OLIGOMERS ; PROTOFIBRILS ; PATHOGENESIS ; AGGREGATION ; MODEL
英文摘要:

The oligomers of A beta(1-40) peptide in PBS buffer solution were analyzed by SEC and native PAGE, and the trimer of A beta(1.40) was also isolated by SEC. In addition, the effects of the soluble A beta(1.40) trimer on intracellular free calcium (Ca2+) balance of hippocampal neurons of postnatal rats were investigated by fluorescence microscopy. The experimental results indicated that A beta(1.40) peptide existed in the form of low molecular weight oligomers in 0.231 mmol/L fresh A beta(1.40) solution (20 mmol/L sodium phosphate buffer, pH 7.4, 0.02% sodium azide) within 24 h and the soluble trimer was the most abundant species. Both the trimeric and the fibrillar A beta(1-40) were able to increase the intracellular Ca2+ concentration, but the A beta(1-40) trimer caused a gradual rise and the potential was also stronger than that of the fibrils at the same concentration. In addition there were different response modes for trimeric and fibrillar A beta(1-40), meaning that there are different mechanisms of increase in intracellular Ca2+ caused by A beta(1-40).

语种: 英语
WOS记录号: WOS:000237493500011
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57499
Appears in Collections:基础医学院_生物物理学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Separation and analysis of the soluble trimer of A beta(1-40) and its effects on .pdf(1715KB)期刊论文出版稿限制开放 联系获取全文

作者单位: Peking Univ, Sch Basic Med Sci, Dept Biophys, Single Mol & Nanobiol Lab, Beijing 100083, Peoples R China

Recommended Citation:
Zheng Xiaohui,Wang Lijun,Zhang Lan,et al. Separation and analysis of the soluble trimer of A beta(1-40) and its effects on the rise in intracellular calcium[J]. CHINESE SCIENCE BULLETIN,2006,51(7):830-838.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zheng Xiaohui]'s Articles
[Wang Lijun]'s Articles
[Zhang Lan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zheng Xiaohui]‘s Articles
[Wang Lijun]‘s Articles
[Zhang Lan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace