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Fibronectin enhances in vitro vascular calcification by promoting osteoblastic differentiation of vascular smooth muscle cells via ERK pathway
Bing, Hai-Tao; Wang, Chen-Guang; Zhang, Tian-Lan; Wang, Kui
关键词Fibronectin Vascular Smooth Muscle Cell Calcification Differentiation Erk Pathway
刊名JOURNAL OF CELLULAR BIOCHEMISTRY
2006-12-01
DOI10.1002/jcb.20999
99期:5页:1343-1352
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]OSTEOPONTIN MESSENGER-RNA ; TRANSCRIPTION FACTOR ; ATHEROSCLEROTIC PLAQUES ; GENE-EXPRESSION ; BONE-FORMATION ; MATRIX ; CBFA1 ; INDUCTION ; LINEAGE ; PROTEIN
英文摘要

The process of vascular calcification presents several features similar to osteogenesis in which fibronectin (FN) acts as a regulator of osteoblastic differentiation and the ERK signal pathway is involved. In order to find whether FN promotes the osteoblastic differentiation of vascular smooth muscle cells (VSMCs) through the ERK signal pathway, we investigated the effect of FN on the calcification of VSMCs by using an in vitro cell model. VSMCs cultured in plates with FN (0-20 pg/cm(2)) coating were induced to calcify by 10 mM sodium beta-glycerophosphate (beta-GP). FN exacerbated VSMC calcification in a dose- and time-dependent manner, as indicated by the number of calcifying nodules per slide and the amount of calcium in, the deposition. Data from RT-PCR and immunoblotting assay revealed that FN also enhanced the expression of several phenotypic markers of osteoblasts, including alkaline phosphatase (ALP) activity, osteocalcin (OC), and Osf2/Cbfa1, a key transcription factor in osteoblastic differentiation. Furthermore, a specific inhibitor for ERK, PD98059 (10 mu M), significantly suppressed the effect of FN on calcification and phenotypic marker expression. These findings seem to suggest that FN enhanced vascular calcification by promoting the osteoblastic differentiation of VSMCs via ERK signal pathway.

语种英语
WOS记录号WOS:000241976100011
引用统计
被引频次:35[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57503
专题北京大学药学院_化学生物学系
作者单位1.Capital Univ Med Sci, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100069, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, Dept Biol Chem, Beijing 100083, Peoples R China
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GB/T 7714
Bing, Hai-Tao,Wang, Chen-Guang,Zhang, Tian-Lan,et al. Fibronectin enhances in vitro vascular calcification by promoting osteoblastic differentiation of vascular smooth muscle cells via ERK pathway[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2006,99(5):1343-1352.
APA Bing, Hai-Tao,Wang, Chen-Guang,Zhang, Tian-Lan,&Wang, Kui.(2006).Fibronectin enhances in vitro vascular calcification by promoting osteoblastic differentiation of vascular smooth muscle cells via ERK pathway.JOURNAL OF CELLULAR BIOCHEMISTRY,99(5),1343-1352.
MLA Bing, Hai-Tao,et al."Fibronectin enhances in vitro vascular calcification by promoting osteoblastic differentiation of vascular smooth muscle cells via ERK pathway".JOURNAL OF CELLULAR BIOCHEMISTRY 99.5(2006):1343-1352.
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