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MicroRNA-1 regulates the proliferation of vascular smooth muscle cells by targeting insulin-like growth factor 1
Liu, Kun1; Ying, Zhang2; Qi, Xia3; Shi, Ying4; Tang, Qiang1
关键词Microrna-1 Insulin-like Growth Factor 1 Hypertension Vascular Smooth Muscle Cells Proliferation
刊名INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
2015-09-01
DOI10.3892/ijmm.2015.2277
36期:3页:817-824
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]FACTOR-I ; ATHEROSCLEROTIC PLAQUES ; SIGNALING PATHWAYS ; KINASE ACTIVATION ; INDUCED APOPTOSIS ; LUNG-CANCER ; EXPRESSION ; INHIBITION ; BINDING ; STRESS
英文摘要

The aim of this study was to investigate the role of microRNAs (miRNAs or miRs) in vascular smooth muscle cell (VSMC) proliferation and to elucidate the underlying molecular mechanisms. In a previous study, using microarray analysis, differentially expressed miRNAs were identified in primary VSMCs isolated from the medial layer of the thoracic aorta obtained from spontaneously hypertensive rats (SHRs) and Wistar Kyoto (WKY) rats. Among others, miR-1 was identified to be downregulated in VSMCs from SHRs. Thus, in the present study, we focused on miR-1, the downregulation of which was confirmed by RT-qPCR and western blot analysis in VSMCs isolated from SHRs. We identified insulin-like growth factor 1 (IGF1) as a potential target gene of miR-1, and we subsequently validated IGF1 as a target gene of miR-1 by luciferase assay. The results revealed that the exogenous overexpression of miR-1 significantly suppressed the expression of IGF1. Additionally, we demonstrated that the downregulation of IGF1 by the introduction of miR-1 attenuated the proliferation of the VSMCs, suggesting that IGF1 is a target gene of miR-1 and that the effects of miR-1 are mediated through IGF1. In conclusion, the findings of our study demonstrate that miR-1 is significantly downregulated in VSMCs and that it is an important regulator of cell proliferation. Therefore, IGF1 may be involved in the regulation of VSMC proliferation by targeting miR-1.

语种英语
WOS记录号WOS:000361134700025
项目编号81100288
资助机构National Natural Science Foundation of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57505
专题北京大学首钢医院_心血管内科
作者单位1.Beijing Youan Hosp, Beijing, Peoples R China
2.Peking Univ, Shougang Hosp, Dept Vasc Med, Beijing 100144, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dept Cardiol, Dalian, Liaoning, Peoples R China
4.Dalian Med Univ, Dept Clin Lab, Transfus Med Sect, Dalian, Liaoning, Peoples R China
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GB/T 7714
Liu, Kun,Ying, Zhang,Qi, Xia,et al. MicroRNA-1 regulates the proliferation of vascular smooth muscle cells by targeting insulin-like growth factor 1[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2015,36(3):817-824.
APA Liu, Kun,Ying, Zhang,Qi, Xia,Shi, Ying,&Tang, Qiang.(2015).MicroRNA-1 regulates the proliferation of vascular smooth muscle cells by targeting insulin-like growth factor 1.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,36(3),817-824.
MLA Liu, Kun,et al."MicroRNA-1 regulates the proliferation of vascular smooth muscle cells by targeting insulin-like growth factor 1".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 36.3(2015):817-824.
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