北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学首钢医院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
MicroRNA-1 regulates the proliferation of vascular smooth muscle cells by targeting insulin-like growth factor 1
作者: Liu, Kun1; Ying, Zhang2; Qi, Xia3; Shi, Ying4; Tang, Qiang1
关键词: microRNA-1 ; insulin-like growth factor 1 ; hypertension ; vascular smooth muscle cells ; proliferation
刊名: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
发表日期: 2015-09-01
DOI: 10.3892/ijmm.2015.2277
卷: 36, 期:3, 页:817-824
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: FACTOR-I ; ATHEROSCLEROTIC PLAQUES ; SIGNALING PATHWAYS ; KINASE ACTIVATION ; INDUCED APOPTOSIS ; LUNG-CANCER ; EXPRESSION ; INHIBITION ; BINDING ; STRESS
英文摘要:

The aim of this study was to investigate the role of microRNAs (miRNAs or miRs) in vascular smooth muscle cell (VSMC) proliferation and to elucidate the underlying molecular mechanisms. In a previous study, using microarray analysis, differentially expressed miRNAs were identified in primary VSMCs isolated from the medial layer of the thoracic aorta obtained from spontaneously hypertensive rats (SHRs) and Wistar Kyoto (WKY) rats. Among others, miR-1 was identified to be downregulated in VSMCs from SHRs. Thus, in the present study, we focused on miR-1, the downregulation of which was confirmed by RT-qPCR and western blot analysis in VSMCs isolated from SHRs. We identified insulin-like growth factor 1 (IGF1) as a potential target gene of miR-1, and we subsequently validated IGF1 as a target gene of miR-1 by luciferase assay. The results revealed that the exogenous overexpression of miR-1 significantly suppressed the expression of IGF1. Additionally, we demonstrated that the downregulation of IGF1 by the introduction of miR-1 attenuated the proliferation of the VSMCs, suggesting that IGF1 is a target gene of miR-1 and that the effects of miR-1 are mediated through IGF1. In conclusion, the findings of our study demonstrate that miR-1 is significantly downregulated in VSMCs and that it is an important regulator of cell proliferation. Therefore, IGF1 may be involved in the regulation of VSMC proliferation by targeting miR-1.

语种: 英语
所属项目编号: 81100288
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000361134700025
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57505
Appears in Collections:北京大学首钢医院_心血管内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Beijing Youan Hosp, Beijing, Peoples R China
2.Peking Univ, Shougang Hosp, Dept Vasc Med, Beijing 100144, Peoples R China
3.Dalian Med Univ, Affiliated Hosp 1, Dept Cardiol, Dalian, Liaoning, Peoples R China
4.Dalian Med Univ, Dept Clin Lab, Transfus Med Sect, Dalian, Liaoning, Peoples R China

Recommended Citation:
Liu, Kun,Ying, Zhang,Qi, Xia,et al. MicroRNA-1 regulates the proliferation of vascular smooth muscle cells by targeting insulin-like growth factor 1[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2015,36(3):817-824.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, Kun]'s Articles
[Ying, Zhang]'s Articles
[Qi, Xia]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, Kun]‘s Articles
[Ying, Zhang]‘s Articles
[Qi, Xia]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace