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学科主题临床医学
Protein P37 of mycoplasma hyorhinis induces secretion of TNF-alpha from human peripheral blood mononuclear cells
Ning, JY; Huang, S; Wu, J; Meng, L; Shou, CC
关键词M. Hyorhinis P37 Protein Induction Peripheral Blood Mononuclear Cell Tnf-alpha
刊名CHINESE SCIENCE BULLETIN
2003-04-01
48期:7页:658-662
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]TUMOR-NECROSIS-FACTOR ; MALIGNANT TRANSFORMATION ; HUMAN-MONOCYTES ; METASTASIS ; CARCINOGENESIS ; INVASIVENESS ; INFECTIONS ; PNEUMONIAE ; INHIBITION ; SEQUENCE
英文摘要

High mycoplasmal infection ratio in gastric cancer tissues suggests a possible association between mycoplasma infection and tumorigenesis. Because TNF-alpha plays an important role in carcinogenesis caused by microbes infection and P37 is a major immunogen of mycoplasma hyorhinis (M. hyor.), investigating whether P37 could induce expression and secretion of TNF-alpha will be very significant to elucidate the possible molecular mechanism of gastric carcinogenesis involved with M. hyor. At the present study, we cloned full gene of p37 by PCR and mutated the 7 codes of TGA into TGG firstly, then expressed the P37 protein successfully with pGEX-4T-1 vector in E. coli, which was verified with Western blot. By RT-PCR and sensitive L929 cell toxic assay, we found that P37 protein could induce expression and secretion of TNF-alpha from human peripheral blood mononuclear cells, and the inducing activity of P37 could be dramatically blocked by McAb PD4. These results suggest that the induction of TNF-alpha secretion by P37 probably plays an important role in diseases caused by M. hyor. infection and needs to be further investigated.

语种英语
WOS记录号WOS:000182411500012
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57514
专题北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Dept Biochem & Mol Biochem, Beijing 100034, Peoples R China
2.Beijing Inst Canc Res, Beijing 100034, Peoples R China
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GB/T 7714
Ning, JY,Huang, S,Wu, J,et al. Protein P37 of mycoplasma hyorhinis induces secretion of TNF-alpha from human peripheral blood mononuclear cells[J]. CHINESE SCIENCE BULLETIN,2003,48(7):658-662.
APA Ning, JY,Huang, S,Wu, J,Meng, L,&Shou, CC.(2003).Protein P37 of mycoplasma hyorhinis induces secretion of TNF-alpha from human peripheral blood mononuclear cells.CHINESE SCIENCE BULLETIN,48(7),658-662.
MLA Ning, JY,et al."Protein P37 of mycoplasma hyorhinis induces secretion of TNF-alpha from human peripheral blood mononuclear cells".CHINESE SCIENCE BULLETIN 48.7(2003):658-662.
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