北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
RACK1 Promotes Autophagy by Enhancing the Atg14L-Beclin1-Vps34-Vps15 Complex Formation upon Phosphorylation by AMPK
作者: Zhao, Yawei1,2; Wang, Qingyang1; Qiu, Guihua1; Zhou, Silei1,2; Jing, Zhaofei1; Wang, Jingyang1; Wang, Wendie1,2; Cao, Junxia1; Han, Kun1; Cheng, Qianqian1; Shen, Beifen1; Chen, Yingyu3; Zhang, Weiping J.4; Ma, Yuanfang2; Zhang, Jiyan1
刊名: CELL REPORTS
发表日期: 2015-11-17
DOI: 10.1016/j.celrep.2015.10.011
卷: 13, 期:7, 页:1407-1417
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: HEPATOCELLULAR-CARCINOMA ; REGULATE AUTOPHAGY ; KINASE ; CELLS ; LIVER ; GROWTH ; MICE ; PROTEINS ; PATHWAY ; STRESS
英文摘要:

Autophagy is essential for maintaining tissue homeostasis. Although adaptors have been demonstrated to facilitate the assembly of the Atg14L-Beclin 1-Vps34-Vps15 complex, which functions in autophagosome formation, it remains unknown whether the autophagy machinery actively recruits such adaptors. WD40-repeat proteins are a large, highly conserved family of adaptors implicated in various cellular activities. However, the role of WD40-repeat-only proteins, such as RACK1, in postnatal mammalian physiology remains unknown. Here, we report that hepatocyte specific RACK1 deficiency leads to lipid accumulation in the liver, accompanied by impaired Atg14L-linked Vps34 activity and autophagy. Further exploration indicates that RACK1 participates in the formation of autophagosome biogenesis complex upon its phosphorylation by AMPK at Thr50. Thr50 phosphorylation of RACK1 enhances its direct binding to Vps15, Atg14L, and Beclin 1, thereby promoting the assembly of the autophagy-initiation complex. These observations provide insight into autophagy induction and establish a pivotal role for RACK1 in postnatal mammalian physiology.

语种: 英语
所属项目编号: 81472736 ; 31270960
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000364990700013
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57533
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
PIIS2211124715011651.pdf(4161KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Inst Basic Med Sci, Dept Mol Immunol, Beijing 100850, Peoples R China
2.Henan Univ, Key Lab Cellular & Mol Immunol, Kaifeng 475001, Henan, Peoples R China
3.Peking Univ, Key Lab Med Immunol, Minist Hlth, Hlth Sci Ctr, Beijing 100083, Peoples R China
4.Second Mil Med Univ, Dept Pathophysiol, Shanghai 200433, Peoples R China

Recommended Citation:
Zhao, Yawei,Wang, Qingyang,Qiu, Guihua,et al. RACK1 Promotes Autophagy by Enhancing the Atg14L-Beclin1-Vps34-Vps15 Complex Formation upon Phosphorylation by AMPK[J]. CELL REPORTS,2015,13(7):1407-1417.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Yawei]'s Articles
[Wang, Qingyang]'s Articles
[Qiu, Guihua]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Yawei]‘s Articles
[Wang, Qingyang]‘s Articles
[Qiu, Guihua]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace