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学科主题基础医学
RACK1 Promotes Autophagy by Enhancing the Atg14L-Beclin1-Vps34-Vps15 Complex Formation upon Phosphorylation by AMPK
Zhao, Yawei1,2; Wang, Qingyang1; Qiu, Guihua1; Zhou, Silei1,2; Jing, Zhaofei1; Wang, Jingyang1; Wang, Wendie1,2; Cao, Junxia1; Han, Kun1; Cheng, Qianqian1; Shen, Beifen1; Chen, Yingyu3; Zhang, Weiping J.4; Ma, Yuanfang2; Zhang, Jiyan1
刊名CELL REPORTS
2015-11-17
DOI10.1016/j.celrep.2015.10.011
13期:7页:1407-1417
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
研究领域[WOS]Cell Biology
关键词[WOS]HEPATOCELLULAR-CARCINOMA ; REGULATE AUTOPHAGY ; KINASE ; CELLS ; LIVER ; GROWTH ; MICE ; PROTEINS ; PATHWAY ; STRESS
英文摘要

Autophagy is essential for maintaining tissue homeostasis. Although adaptors have been demonstrated to facilitate the assembly of the Atg14L-Beclin 1-Vps34-Vps15 complex, which functions in autophagosome formation, it remains unknown whether the autophagy machinery actively recruits such adaptors. WD40-repeat proteins are a large, highly conserved family of adaptors implicated in various cellular activities. However, the role of WD40-repeat-only proteins, such as RACK1, in postnatal mammalian physiology remains unknown. Here, we report that hepatocyte specific RACK1 deficiency leads to lipid accumulation in the liver, accompanied by impaired Atg14L-linked Vps34 activity and autophagy. Further exploration indicates that RACK1 participates in the formation of autophagosome biogenesis complex upon its phosphorylation by AMPK at Thr50. Thr50 phosphorylation of RACK1 enhances its direct binding to Vps15, Atg14L, and Beclin 1, thereby promoting the assembly of the autophagy-initiation complex. These observations provide insight into autophagy induction and establish a pivotal role for RACK1 in postnatal mammalian physiology.

语种英语
WOS记录号WOS:000364990700013
项目编号81472736 ; 31270960
资助机构National Natural Science Foundation of China
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57533
专题北京大学基础医学院_免疫学系
北京大学基础医学院
作者单位1.Inst Basic Med Sci, Dept Mol Immunol, Beijing 100850, Peoples R China
2.Henan Univ, Key Lab Cellular & Mol Immunol, Kaifeng 475001, Henan, Peoples R China
3.Peking Univ, Key Lab Med Immunol, Minist Hlth, Hlth Sci Ctr, Beijing 100083, Peoples R China
4.Second Mil Med Univ, Dept Pathophysiol, Shanghai 200433, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Yawei,Wang, Qingyang,Qiu, Guihua,et al. RACK1 Promotes Autophagy by Enhancing the Atg14L-Beclin1-Vps34-Vps15 Complex Formation upon Phosphorylation by AMPK[J]. CELL REPORTS,2015,13(7):1407-1417.
APA Zhao, Yawei.,Wang, Qingyang.,Qiu, Guihua.,Zhou, Silei.,Jing, Zhaofei.,...&Zhang, Jiyan.(2015).RACK1 Promotes Autophagy by Enhancing the Atg14L-Beclin1-Vps34-Vps15 Complex Formation upon Phosphorylation by AMPK.CELL REPORTS,13(7),1407-1417.
MLA Zhao, Yawei,et al."RACK1 Promotes Autophagy by Enhancing the Atg14L-Beclin1-Vps34-Vps15 Complex Formation upon Phosphorylation by AMPK".CELL REPORTS 13.7(2015):1407-1417.
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