|Key molecular events in puromycin aminonucleoside nephrosis rats|
|Guan, N; Ding, J; Deng, JH; Zhang, JJ; Yang, JY
|WOS标题词||Science & Technology
; SLIT DIAPHRAGM
; DEVELOPING GLOMERULI
; PROTEINURIC STATES
Nephrin, podocin and alpha-actinin are all involved in proteinuria, but it is unclear which molecular event plays a crucial role during the development of proteinuria. Immunofluorescence staining and real-time quantitative polymerase chain reaction were used to study the glomerular expression of these molecules in puromycin aminonucleoside (PAN) nephrosis. Morphometric methods were applied to evaluate the podocyte foot process (FP) morphology. Two days after PAN injection, nephrin and podocin staining became discontinuous, podocin intensity decreased and FP swelled. Nephrin protein and mRNA decreased at day 5. Both podocin and nephrin intensity decreased dramatically when heavy proteinuria occurred, but nephrin mRNA was regained. When proteinuria disappeared, podocin recovered whereas nephrin did not (P=0.02); alpha-actinin intensity increased (P=0.009) and the distribution changed. The podocyte FP volume density correlated negatively with nephrin (r=-0.78, P=0.0001) and podocin immunofluorescence intensity (r=-0.76, P=0.0001). We conclude that, before the onset of proteinuria, the first response was the nephrin and podocin distribution change, podocin protein decrease and swollen FP; the podocin quantitative change was earlier than nephrin. Podocin and nephrin distribution and the protein level was associated with proteinuria more closely than their mRNA level. The delayed alpha-actinin induction might be a reparative response.
|作者单位||Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China|
Guan, N,Ding, J,Deng, JH,et al. Key molecular events in puromycin aminonucleoside nephrosis rats[J]. PATHOLOGY INTERNATIONAL,2004,54(9):703-711.
Guan, N,Ding, J,Deng, JH,Zhang, JJ,&Yang, JY.(2004).Key molecular events in puromycin aminonucleoside nephrosis rats.PATHOLOGY INTERNATIONAL,54(9),703-711.
Guan, N,et al."Key molecular events in puromycin aminonucleoside nephrosis rats".PATHOLOGY INTERNATIONAL 54.9(2004):703-711.
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