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学科主题临床医学
Key molecular events in puromycin aminonucleoside nephrosis rats
Guan, N; Ding, J; Deng, JH; Zhang, JJ; Yang, JY
关键词Alpha-actinin Molecular Event Morphometry Nephrin Podocin Proteinuria
刊名PATHOLOGY INTERNATIONAL
2004-09-01
54期:9页:703-711
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pathology
研究领域[WOS]Pathology
关键词[WOS]PODOCYTE-ASSOCIATED MOLECULES ; SLIT DIAPHRAGM ; ALPHA-ACTININ ; DEVELOPING GLOMERULI ; PROTEINURIC STATES ; NEPHRIN ; EXPRESSION ; COMPONENT ; CLONING ; KIDNEY
英文摘要

Nephrin, podocin and alpha-actinin are all involved in proteinuria, but it is unclear which molecular event plays a crucial role during the development of proteinuria. Immunofluorescence staining and real-time quantitative polymerase chain reaction were used to study the glomerular expression of these molecules in puromycin aminonucleoside (PAN) nephrosis. Morphometric methods were applied to evaluate the podocyte foot process (FP) morphology. Two days after PAN injection, nephrin and podocin staining became discontinuous, podocin intensity decreased and FP swelled. Nephrin protein and mRNA decreased at day 5. Both podocin and nephrin intensity decreased dramatically when heavy proteinuria occurred, but nephrin mRNA was regained. When proteinuria disappeared, podocin recovered whereas nephrin did not (P=0.02); alpha-actinin intensity increased (P=0.009) and the distribution changed. The podocyte FP volume density correlated negatively with nephrin (r=-0.78, P=0.0001) and podocin immunofluorescence intensity (r=-0.76, P=0.0001). We conclude that, before the onset of proteinuria, the first response was the nephrin and podocin distribution change, podocin protein decrease and swollen FP; the podocin quantitative change was earlier than nephrin. Podocin and nephrin distribution and the protein level was associated with proteinuria more closely than their mRNA level. The delayed alpha-actinin induction might be a reparative response.

语种英语
WOS记录号WOS:000223642400008
引用统计
被引频次:30[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57545
专题北京大学第一临床医学院_儿科
作者单位Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China
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GB/T 7714
Guan, N,Ding, J,Deng, JH,et al. Key molecular events in puromycin aminonucleoside nephrosis rats[J]. PATHOLOGY INTERNATIONAL,2004,54(9):703-711.
APA Guan, N,Ding, J,Deng, JH,Zhang, JJ,&Yang, JY.(2004).Key molecular events in puromycin aminonucleoside nephrosis rats.PATHOLOGY INTERNATIONAL,54(9),703-711.
MLA Guan, N,et al."Key molecular events in puromycin aminonucleoside nephrosis rats".PATHOLOGY INTERNATIONAL 54.9(2004):703-711.
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