IR@PKUHSC  > 北京大学药学院  > 化学生物学系
学科主题药学
Vanadyl ions binding to GroEL (HSP60) and inducing its depolymerization
Lei WanHua; Liu HuiXue; Zhong LiJun; Yang XiaoDa; Wang Kui
关键词Vanadium Mitochondria Hsp60 Diabetes
刊名CHINESE SCIENCE BULLETIN
2007-10-01
DOI10.1007/s11434-007-0380-0
52期:20页:2775-2781
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]PANCREATIC BETA-CELLS ; MITOCHONDRIAL PROTEINS ; ATP HYDROLYSIS ; DIABETIC RATS ; INSULIN ; MECHANISM ; PHOSPHATE ; APOPTOSIS ; SITE ; VANADIUM(IV)
英文摘要

Several vanadium compounds have been known for the hypoglycemic and anticancer effects. However, the mechanisms of the pharmacological and toxicological effects were not clear. In this work, we investigated the potential targets of vanadium in mitochondria. Vanadyl ions were found to bind to mitochondria from rat liver with a stoichiometry of 244 +/- 58 nmol/mg protein and an apparent dissociation constant (K (d)) of (2.0 +/- 0.8)x10(-16) mol/L. Using size exclusion chromatography, a vanadium-binding protein was isolated and identified to be the 60-kDa heat shock protein (HSP60) by mass spectrometry analysis and immunoassays. Additionally, binding of vanadyl ions was found to result in depolymerization of homo-oligomeric HSP60 (GroEL). HSP60 is an indispensable molecular chaperone and involved in many kinds of pathogenesis of inflammatory and autoimmune diseases, e.g. type 1 diabetes. Our results suggested that HSP60 could be a novel important target involved in the biological and/or toxicological effects of vanadium compounds.

语种英语
WOS记录号WOS:000249986200007
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57549
专题北京大学药学院_化学生物学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Med & Hlth Anal Ctr, Beijing 100083, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Natl Lab Nat & Biomimetr Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Lei WanHua,Liu HuiXue,Zhong LiJun,et al. Vanadyl ions binding to GroEL (HSP60) and inducing its depolymerization[J]. CHINESE SCIENCE BULLETIN,2007,52(20):2775-2781.
APA Lei WanHua,Liu HuiXue,Zhong LiJun,Yang XiaoDa,&Wang Kui.(2007).Vanadyl ions binding to GroEL (HSP60) and inducing its depolymerization.CHINESE SCIENCE BULLETIN,52(20),2775-2781.
MLA Lei WanHua,et al."Vanadyl ions binding to GroEL (HSP60) and inducing its depolymerization".CHINESE SCIENCE BULLETIN 52.20(2007):2775-2781.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lei WanHua]的文章
[Liu HuiXue]的文章
[Zhong LiJun]的文章
百度学术
百度学术中相似的文章
[Lei WanHua]的文章
[Liu HuiXue]的文章
[Zhong LiJun]的文章
必应学术
必应学术中相似的文章
[Lei WanHua]的文章
[Liu HuiXue]的文章
[Zhong LiJun]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。