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学科主题: 药学
题名:
Vanadyl ions binding to GroEL (HSP60) and inducing its depolymerization
作者: Lei WanHua; Liu HuiXue; Zhong LiJun; Yang XiaoDa; Wang Kui
关键词: vanadium ; mitochondria ; HSP60 ; diabetes
刊名: CHINESE SCIENCE BULLETIN
发表日期: 2007-10-01
DOI: 10.1007/s11434-007-0380-0
卷: 52, 期:20, 页:2775-2781
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: PANCREATIC BETA-CELLS ; MITOCHONDRIAL PROTEINS ; ATP HYDROLYSIS ; DIABETIC RATS ; INSULIN ; MECHANISM ; PHOSPHATE ; APOPTOSIS ; SITE ; VANADIUM(IV)
英文摘要:

Several vanadium compounds have been known for the hypoglycemic and anticancer effects. However, the mechanisms of the pharmacological and toxicological effects were not clear. In this work, we investigated the potential targets of vanadium in mitochondria. Vanadyl ions were found to bind to mitochondria from rat liver with a stoichiometry of 244 +/- 58 nmol/mg protein and an apparent dissociation constant (K (d)) of (2.0 +/- 0.8)x10(-16) mol/L. Using size exclusion chromatography, a vanadium-binding protein was isolated and identified to be the 60-kDa heat shock protein (HSP60) by mass spectrometry analysis and immunoassays. Additionally, binding of vanadyl ions was found to result in depolymerization of homo-oligomeric HSP60 (GroEL). HSP60 is an indispensable molecular chaperone and involved in many kinds of pathogenesis of inflammatory and autoimmune diseases, e.g. type 1 diabetes. Our results suggested that HSP60 could be a novel important target involved in the biological and/or toxicological effects of vanadium compounds.

语种: 英语
WOS记录号: WOS:000249986200007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57549
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Dept Biol Chem, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Med & Hlth Anal Ctr, Beijing 100083, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, Hlth Sci Ctr, Natl Lab Nat & Biomimetr Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Lei WanHua,Liu HuiXue,Zhong LiJun,et al. Vanadyl ions binding to GroEL (HSP60) and inducing its depolymerization[J]. CHINESE SCIENCE BULLETIN,2007,52(20):2775-2781.
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