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学科主题基础医学
Detection of Newcastle disease virus using nucleic acid sequence-based amplification
Cui, Shang-Jin; Fung, Yin-Wan Wendy; Lau, Lok Ting; Liu, Wen-Bin; Wang, Yun-Feng; Tong, Guang-Zhi; Chen, Jianguo; Yu, Albert Cheung Hoi
关键词Newcastle Disease Nasba Ndv Rna Amplicons Surveillance Disease Screening
刊名BIOLOGICALS
2007-03-01
DOI10.1016/j.biologicals.2005.12.002
35期:1页:13-18
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
关键词[WOS]REVERSE-TRANSCRIPTION-PCR ; QUANTIFICATION ; NASBA ; RNA
英文摘要

Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance. (c) 2005 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000244956600003
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57556
专题北京大学基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Minist Educ, Key Lab Neurosci PKU, Beijing 100083, Peoples R China
3.CAAS, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Harbin 150001, Peoples R China
4.Hong Kong DNA Chips Ltd, Hang Tung Resources Ctr 8F, Hong Kong, Hong Kong, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100083, Peoples R China
6.Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Cui, Shang-Jin,Fung, Yin-Wan Wendy,Lau, Lok Ting,et al. Detection of Newcastle disease virus using nucleic acid sequence-based amplification[J]. BIOLOGICALS,2007,35(1):13-18.
APA Cui, Shang-Jin.,Fung, Yin-Wan Wendy.,Lau, Lok Ting.,Liu, Wen-Bin.,Wang, Yun-Feng.,...&Yu, Albert Cheung Hoi.(2007).Detection of Newcastle disease virus using nucleic acid sequence-based amplification.BIOLOGICALS,35(1),13-18.
MLA Cui, Shang-Jin,et al."Detection of Newcastle disease virus using nucleic acid sequence-based amplification".BIOLOGICALS 35.1(2007):13-18.
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