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学科主题: 基础医学
题名:
Detection of Newcastle disease virus using nucleic acid sequence-based amplification
作者: Cui, Shang-Jin; Fung, Yin-Wan Wendy; Lau, Lok Ting; Liu, Wen-Bin; Wang, Yun-Feng; Tong, Guang-Zhi; Chen, Jianguo; Yu, Albert Cheung Hoi
关键词: Newcastle disease ; NASBA ; NDV ; RNA amplicons ; surveillance ; disease screening
刊名: BIOLOGICALS
发表日期: 2007-03-01
DOI: 10.1016/j.biologicals.2005.12.002
卷: 35, 期:1, 页:13-18
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
研究领域[WOS]: Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Pharmacology & Pharmacy
关键词[WOS]: REVERSE-TRANSCRIPTION-PCR ; QUANTIFICATION ; NASBA ; RNA
英文摘要:

Newcastle disease (ND) is a contagious and widespread avian disease affecting most species of birds. ND virus (NDV) is the only member of the avian paramyxovirus serotype 1 (APMV1) causing ND outbreak in bird flocks. The technique of nucleic acid sequence-based amplification (NASBA) is a potential method to rapidly and reliably detect NDV isolates. Here, we describe an effective and unprecedented method for detecting NDV strains of all pathotypes. A conserved region of the fusion protein gene was used for designing oligonucleotides specific to all NDV pathotypes. The dynamic range of this NDV NASBA detection method is comparable to virus culture and therefore the NDV NASBA method is a potential alternative for NDV screening and surveillance. (c) 2005 The International Association for Biologicals. Published by Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000244956600003
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57556
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100083, Peoples R China
2.Minist Educ, Key Lab Neurosci PKU, Beijing 100083, Peoples R China
3.CAAS, Harbin Vet Res Inst, Natl Key Lab Vet Biotechnol, Harbin 150001, Peoples R China
4.Hong Kong DNA Chips Ltd, Hang Tung Resources Ctr 8F, Hong Kong, Hong Kong, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Neurobiol, Beijing 100083, Peoples R China
6.Peking Univ, Coll Life Sci, Dept Cell Biol & Genet, Beijing 100871, Peoples R China

Recommended Citation:
Cui, Shang-Jin,Fung, Yin-Wan Wendy,Lau, Lok Ting,et al. Detection of Newcastle disease virus using nucleic acid sequence-based amplification[J]. BIOLOGICALS,2007,35(1):13-18.
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