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学科主题: 临床医学
题名:
L-Cystathionine Inhibits the Mitochondria-Mediated Macrophage Apoptosis Induced by Oxidized Low Density Lipoprotein
作者: Zhu, Mingzhu1; Du, Junbao1,2; Chen, Siyao1; Liu, Angie Dong3; Holmberg, Lukas3; Chen, Yonghong1; Zhang, Chunyu1; Tang, Chaoshu2,4; Jin, Hongfang1
关键词: L-cystathionine ; ox-LDL ; macrophage ; mitochondrial ; oxidative stress ; apoptosis ; caspase-9 ; caspase-3
刊名: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
发表日期: 2014-12-01
DOI: 10.3390/ijms151223059
卷: 15, 期:12, 页:23059-23073
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Chemistry, Multidisciplinary
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CYTOCHROME-C ; OXIDATIVE STRESS ; CELL-DEATH ; METABOLISM ; ATHEROSCLEROSIS ; PATHWAY ; PROTEIN ; NEUROBLASTOMA ; NEUTROPHILS ; EXPRESSION
英文摘要:

This study was designed to investigate the regulatory role of L-cystathionine in human macrophage apoptosis induced by oxidized low density lipoprotein (ox-LDL) and its possible mechanisms. THP-1 cells were induced with phorbol 12-myristate 13-acetate (PMA) and differentiated into macrophages. Macrophages were incubated with ox-LDL after pretreatment with L-cystathionine. Superoxide anion, apoptosis, mitochondrial membrane potential, and mitochondrial permeability transition pore (MPTP) opening were examined. Caspase-9 activities and expression of cleaved caspase-3 were measured. The results showed that compared with control group, ox-LDL treatment significantly promoted superoxide anion generation, release of cytochrome c (cytc) from mitochondrion into cytoplasm, caspase-9 activities, cleavage of caspase-3, and cell apoptosis, in addition to reduced mitochondrial membrane potential as well as increased MPTP opening. However, 0.3 and 1.0 mmol/L L-cystathionine significantly reduced superoxide anion generation, increased mitochondrial membrane potential, and markedly decreased MPTP opening in ox-LDL + L-cystathionine macrophages. Moreover, compared to ox-LDL treated-cells, release of cytc from mitochondrion into cytoplasm, caspase-9 activities, cleavage of caspase-3, and apoptosis levels in L-cystathionine pretreated cells were profoundly attenuated. Taken together, our results suggested that L-cystathionine could antagonize mitochondria-mediated human macrophage apoptosis induced by ox-LDL via inhibition of cytc release and caspase activation.

语种: 英语
所属项目编号: 2012CB517806 ; 2013CB933801 ; 2011CB503904 ; 31130030 ; 81370154 ; 81121061 ; 20130001120047 ; 7121014 ; 2014XXGB02
项目资助者: Major Basic Research Development Program of People&prime ; s Republic of China ; National Natural Science Foundation of China ; Grant of Ministry of Education, China ; Beijing Natural Science Foundation ; Key Laboratory of Remodeling-related Cardiovascular Diseases, Ministry of Education
WOS记录号: WOS:000346797400089
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57558
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Minist Educ, Key Lab Mol Cardiol, Beijing 100191, Peoples R China
2.Linkoping Univ, Dept Med & Hlth Sci, S-58183 Linkoping, Sweden
3.Peking Univ, Dept Pediat, Hosp 1, Beijing 100034, Peoples R China
4.Peking Univ, Dept Physiol & Pathophysiol, Hlth Sci Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Zhu, Mingzhu,Du, Junbao,Chen, Siyao,et al. L-Cystathionine Inhibits the Mitochondria-Mediated Macrophage Apoptosis Induced by Oxidized Low Density Lipoprotein[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2014,15(12):23059-23073.
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