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学科主题: 临床医学
题名:
Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections: a multicenter study in China
作者: Li De-zhi1; Chen Yu-sheng2; Yang Jing-ping3; Zhang Wei4; Hu Cheng-ping5; Li Jia-shu6; Mu Lan7; Hu Ying-hui8; Geng Rong9; Hu Ke10; Cai Shao-xi11; Wan Huan-ying12; Wang Qiu-yue13; Wei Li-ping14; Du Juan15; Yu Qin16; Zhong Xiao-ning17; Wang Rui-qin18; Ma Jian-jun19; Tian Gui-zhen20; Wang Si-qin21; Gao Zhan-cheng1
关键词: Staphylococcus aureus ; lower respiratory tract infections ; molecular epidemiology ; staphylococcal cassette chromosome mec ; Multilocus Sequence Typing
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2011-03-05
DOI: 10.3760/cma.j.issn.0366-6999.2011.05.010
卷: 124, 期:5, 页:687-692
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: PANTON-VALENTINE LEUKOCIDIN ; CASSETTE CHROMOSOME MEC ; METHICILLIN-RESISTANT ; MULTIPLEX PCR ; IDENTIFICATION ; SURVEILLANCE ; VANCOMYCIN ; PNEUMONIA ; CHILDREN ; STRAINS
英文摘要:

Background Staphylococcus aureus (S. aureus) remains as an important microbial pathogen resulting in community and nosocomial acquired infections with significant morbidity and mortality. Few reports for S. aureus in lower respiratory tract infections (LRTIs) have been documented. The aim of this study was to explore the molecular epidemiology of S. aureus in LRTIs in China.

Methods A multicenter study of the molecular epidemiology of S. aureus in LRTIs was conducted in 21 hospitals in Beijing, Shanghai and twelve other provinces from November 2007 to February 2009. All the collected S. aureus strains were classified as minimum inhibitory concentration (MIC), mecA gene, virulence genes Panton-Valentine Leukocidin (PVL) and y-hemolysin (hlg), staphylococcal cassette chromosome mec (SCCmec) type, agr type, and Multi locus Sequence Typing (MLST).

Results Totally, nine methicillin-sensitive S. aureus (MSSA) and 29 methicillin-resistant S. aureus (MRSA) strains were isolated after culture from a total of 2829 sputums or bronchoalveolar lavages. The majority of MRSA strains (22/29) had a MIC value of >= 512 mu g/ml for cefoxitin. The mecA gene acting as the conservative gene was carried by all MRSA strains. PVL genes were detected in only one S. aureus strain (2.63%, 1/38). The hlg gene was detected in almost the all S. aureus (100% in MSSA and 96.56% in MRSA strains). About 75.86% of MRSA strains carried SCCmec III. Agr type 1 was predominant (78.95%) among the identified three agr types (agr types 1, 2, and 3). Totally, ten sequence type (ST) of S. aureus strains were detected. A new sequence type (ST1445) was found besides confirming ST239 as the major sequence type (60.53%). A dendrogram generated from our own MLST database showed all the bootstrap values <= 50%..

Conclusion Our preliminary epidemiology data show SCCmec III, ST239 and agr type 1 of S. aureus as the predominant strains in LRTIs in Mainland of China. Chin Med J 2011;124(5):687-692

语种: 英语
所属项目编号: 2006AA02Z4A9
项目资助者: National Hi-Tech Project
WOS记录号: WOS:000289040600010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57569
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.Fujian Prov Hosp, Dept Resp Med, Fuzhou 350001, Fujian, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Resp & Crit Care Med, Beijing 100044, Peoples R China
3.Inner Mongolia Med Coll, Affiliated Hosp 3, Dept Resp Med, Baotou 014010, Inner Mongolia, Peoples R China
4.Nanchang Univ, Affiliated Hosp 1, Dept Resp Med, Nanchang 330006, Jiangxi, Peoples R China
5.Cent S Univ, Xiangya Hosp, Dept Resp Med, Changsha 410008, Hunan, Peoples R China
6.Xuzhou Med Coll, Lianyungang Peoples Hosp 1, Dept Resp Med, Lianyungang 222002, Jiangsu, Peoples R China
7.Peking Univ, Space Cent Hosp, Dept Resp Med, Beijing 100049, Peoples R China
8.Capital Med Univ, Beijing Childrens Hosp, Dept Resp Med, Beijing 100045, Peoples R China
9.Capital Med Univ, Beijing Childrens Hosp, Dept Emergency Med, Beijing 100045, Peoples R China
10.Wuhan Univ, Renmin Hosp, Dept Resp Med, Wuhan 430060, Hubei, Peoples R China
11.So Med Univ, Nanfang Hosp, Dept Resp Med, Guangzhou 510515, Guangdong, Peoples R China
12.Shanghai Jiao Tong Univ, Ruijin Hosp, Dept Resp Med, Shanghai 200025, Peoples R China
13.China Med Univ, Affiliated Hosp 1, Inst Resp Dis, Shenyang 110001, Liaoning, Peoples R China
14.Guangzhou Med Univ, Affiliated Hosp 3, Dept Resp Med, Guangzhou 510150, Guangdong, Peoples R China
15.Guiyang Med Coll, Affiliated Hosp, Dept Resp Med, Guiyang 550004, Guizhou, Peoples R China
16.Lanzhou Univ, Hosp 1, Dept Resp Med, Lanzhou 730000, Gansu, Peoples R China
17.Guangxi Med Univ, Affiliated Hosp 1, Dept Resp Med, Nanning 530021, Guangxi, Peoples R China
18.Tsinghua Univ, Affiliated Hosp 1, Dept Resp Med, Beijing 100016, Peoples R China
19.Lanzhou Pulm Hosp, Dept Resp Med, Lanzhou 730046, Gansu, Peoples R China
20.Mil Gen Hosp Beijing, Clin Sect 263, Dept Resp Med, Beijing 101149, Peoples R China
21.Henan Prov People Hosp, Dept Resp Med, Zhengzhou 450003, Henan, Peoples R China

Recommended Citation:
Li De-zhi,Chen Yu-sheng,Yang Jing-ping,et al. Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections: a multicenter study in China[J]. CHINESE MEDICAL JOURNAL,2011,124(5):687-692.
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