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学科主题: 临床医学
题名:
Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer
作者: Zou Shu-li; Chang Xiao-hong; Ye Xue; Cheng Hong-yan; Cheng Ye-xia; Tang Zhi-jian; Zhang Zu-juan; Gao Li; Chen Xin-hua; Cui Heng
关键词: human epididymis protein 4 ; RNA interference ; ovarian carcinoma ; carcinogenesis
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2011-10-05
DOI: 10.3760/cma.j.issn.0366-6999.2011.19.032
卷: 124, 期:19, 页:3133-3140
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: BREAST-CANCER ; EXPRESSION PROFILES ; EPITHELIAL-CELLS ; CDNA MICROARRAY ; CARCINOMAS ; HE-4 ; HYBRIDIZATION ; INHIBITOR ; MARKERS ; DIFFERENTIATION
英文摘要:

Background Human epididymis secretory protein 4 (HE4) has been proved to be a promising novel biomarker for the detection of epithelial ovarian carcinomas. Compared with CA125, HE4 assay demonstrated an improved ability to discriminate between pelvic mass with malignant and benign disease. Though it is well known that HE4 is overexpressed in ovarian cancer, however, the role of HE4 in the carcinogenesis and progression of ovarian cancer remains unkown. Methods In this study, we explored the role of HE4 in the carcinogenesis and progression of ovarian cancer. We screened nine ovarian cancer cell lines for HE4 expression, and using RNA interference (RNAi), we silenced HE4 gene expression in CaoV3 and SKOV3.ip1 ovarian cancer cell lines. We assessed the effect of HE4 gene silencing on the transformed phenotype by examining the cell cycle, apoptosis, proliferation and transwell migration/invasion in vitro. Results HE4 gene silencing induces G0/G1 arrest and blocks the progression from the G1 to S phase in CaoV3 and SKOV3.ip1 cells. HE4 knockdown also inhibited cell proliferation, migration and invasion in SKOV3.ip1 cells in vitro. Conclusion HE4 may be involved in the regulation of the cell cycle and promote ovarian cancer migration and invasion. Chin Med J 2011;124(19):3133-3140

语种: 英语
所属项目编号: 81172454 ; SRFDP20100001110079 ; RDB2010-11
项目资助者: National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Peking University People&prime ; s Hospital
WOS记录号: WOS:000296307600033
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57572
Appears in Collections:北京大学第二临床医学院_妇科肿瘤中心_期刊论文

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作者单位: Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China

Recommended Citation:
Zou Shu-li,Chang Xiao-hong,Ye Xue,et al. Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer[J]. CHINESE MEDICAL JOURNAL,2011,124(19):3133-3140.
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