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Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer
Zou Shu-li; Chang Xiao-hong; Ye Xue; Cheng Hong-yan; Cheng Ye-xia; Tang Zhi-jian; Zhang Zu-juan; Gao Li; Chen Xin-hua; Cui Heng
关键词Human Epididymis Protein 4 Rna Interference Ovarian Carcinoma Carcinogenesis
刊名CHINESE MEDICAL JOURNAL
2011-10-05
DOI10.3760/cma.j.issn.0366-6999.2011.19.032
124期:19页:3133-3140
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]BREAST-CANCER ; EXPRESSION PROFILES ; EPITHELIAL-CELLS ; CDNA MICROARRAY ; CARCINOMAS ; HE-4 ; HYBRIDIZATION ; INHIBITOR ; MARKERS ; DIFFERENTIATION
英文摘要

Background Human epididymis secretory protein 4 (HE4) has been proved to be a promising novel biomarker for the detection of epithelial ovarian carcinomas. Compared with CA125, HE4 assay demonstrated an improved ability to discriminate between pelvic mass with malignant and benign disease. Though it is well known that HE4 is overexpressed in ovarian cancer, however, the role of HE4 in the carcinogenesis and progression of ovarian cancer remains unkown. Methods In this study, we explored the role of HE4 in the carcinogenesis and progression of ovarian cancer. We screened nine ovarian cancer cell lines for HE4 expression, and using RNA interference (RNAi), we silenced HE4 gene expression in CaoV3 and SKOV3.ip1 ovarian cancer cell lines. We assessed the effect of HE4 gene silencing on the transformed phenotype by examining the cell cycle, apoptosis, proliferation and transwell migration/invasion in vitro. Results HE4 gene silencing induces G0/G1 arrest and blocks the progression from the G1 to S phase in CaoV3 and SKOV3.ip1 cells. HE4 knockdown also inhibited cell proliferation, migration and invasion in SKOV3.ip1 cells in vitro. Conclusion HE4 may be involved in the regulation of the cell cycle and promote ovarian cancer migration and invasion. Chin Med J 2011;124(19):3133-3140

语种英语
WOS记录号WOS:000296307600033
项目编号81172454 ; SRFDP20100001110079 ; RDB2010-11
资助机构National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education ; Peking University People&prime ; s Hospital
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57572
专题北京大学第二临床医学院_妇科肿瘤中心
北京大学第二临床医学院_妇科
作者单位Peking Univ, Peoples Hosp, Gynecol Oncol Ctr, Beijing 100044, Peoples R China
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GB/T 7714
Zou Shu-li,Chang Xiao-hong,Ye Xue,et al. Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer[J]. CHINESE MEDICAL JOURNAL,2011,124(19):3133-3140.
APA Zou Shu-li.,Chang Xiao-hong.,Ye Xue.,Cheng Hong-yan.,Cheng Ye-xia.,...&Cui Heng.(2011).Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer.CHINESE MEDICAL JOURNAL,124(19),3133-3140.
MLA Zou Shu-li,et al."Effect of human epididymis protein 4 gene silencing on the malignant phenotype in ovarian cancer".CHINESE MEDICAL JOURNAL 124.19(2011):3133-3140.
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