|Morphology of bacterial flora in root canals associated with apical abscesses|
|Guo Huijie1; Gao Chengzhi1; Zhang Chengfei3; Zheng Shuying2; Yue Lin4|
|关键词||Apical Abscess Biofilm Clinical Symptoms Dentinal Tubules Endodontic Infection|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||DENTINAL TUBULES ; ENDODONTIC INFECTIONS ; PERIODONTITIS ; TEETH ; BIOFILMS|
Background Apical abscess is an inflammatory process in the peri-radicular tissues caused by biofilms in the necrotic root canal systems. Therefore, a comprehensive analysis of the bacterial colonization is required for a better understanding of the pathogenesis. This study aimed to investigate the patterns of bacterial infection of root canals of teeth with apical abscesses and to determine whether histological and microbiological findings correlated with clinical conditions.
Methods Eighteen samples from 18 teeth with apical pathological lesions were analyzed. Nine patients with acute apical abscesses experienced severe pain, and nine patients were asymptomatic with a sinus tract. After extraction, each affected root was divided into two halves. One half was processed for histobacteriologic analysis and examined using light microscopy, and the other half was analyzed using scanning electron microscopy (SEM) to determine the patterns of microbial colonization of the root canals.
Results The appearance of each sample subjected to SEM was consistent with the histobacteriologic findings despite the presence or absence of clinical symptoms. Intraradicular biofilms comprising cocci, rods, and/or filaments of amorphous materials were observed in the apical third of the main root canals in all samples. The bacterial biofilms covering the main root canal walls also penetrated the dentinal tubules to varying depths. The morphologies of biofilms varied, and a unique pattern of intraradicular infection was not identified.
Conclusion Intraradicular infections formed complex and variable multispecies biofilms and their presence did not correlate with clinical symptoms.
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Peking Univ, Peoples Hosp, Dept Stomatol, Beijing 100044, Peoples R China|
2.Peking Univ, Peoples Hosp, Electron Microscopy Lab, Beijing 100044, Peoples R China
3.Univ Hong Kong, Fac Dent, Hong Kong, Hong Kong, Peoples R China
4.Peking Univ, Sch & Hosp Stomatol, Dept Cariol Endodontol & Operat Dent, Beijing 100081, Peoples R China
|Guo Huijie,Gao Chengzhi,Zhang Chengfei,et al. Morphology of bacterial flora in root canals associated with apical abscesses[J]. CHINESE MEDICAL JOURNAL,2014,127(18):3254-3258.|
|APA||Guo Huijie,Gao Chengzhi,Zhang Chengfei,Zheng Shuying,&Yue Lin.(2014).Morphology of bacterial flora in root canals associated with apical abscesses.CHINESE MEDICAL JOURNAL,127(18),3254-3258.|
|MLA||Guo Huijie,et al."Morphology of bacterial flora in root canals associated with apical abscesses".CHINESE MEDICAL JOURNAL 127.18(2014):3254-3258.|