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学科主题临床医学
Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region
Yu, Yang1,2,3; Gao, Qian1; Zhao, Hong-cui1; Li, Rong1,2,3; Gao, Jiang-man1; Ding, Ting1,3; Bao, Si-yu1,2; Zhao, Yue1,3; Sun, Xiao-fang4; Fan, Yong1,4; Qiao, Jie1,2,3
刊名STEM CELL RESEARCH & THERAPY
2015-04-14
DOI10.1186/s13287-015-0054-9
6
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Medicine, Research & Experimental
研究领域[WOS]Cell Biology ; Research & Experimental Medicine
关键词[WOS]NUCLEAR TRANSFER ; IN-VITRO ; METHYLATION STATUS ; LINES ; DIFFERENTIATION ; FERTILIZATION ; BLASTOCYSTS ; ACTIVATION ; GENERATION ; CLUSTER
英文摘要

Introduction: Human parthenogenetic embryonic stem cells (hpESCs) are generated from artificially activated oocytes, however, the issue of whether hpESCs have equivalent differentiation ability to human fertilized embryonic stem cells remains controversial.

Methods: hpESCs were injected into male severe combined immunodeficiency (SCID) mice and the efficiency of teratoma formation was calculated. Then the gene expression and methylation modification were detected by real time-PCR and bisulfate methods.

Results: Comparison of five hpESCs with different differentiation abilities revealed that levels of paternal genes in the Dlk1-Dio3 region on chromosome 14 in the hpESCs with high differentiation potential are enhanced, but strictly methylated and silenced in the hpESCs with lower differentiation potential. Treatment with ascorbic acid, rescued their ability to support teratoma formation and altered the expression profiles of paternally expressed genes in hpESCs that could not form teratoma easily. No differences in the expression of other imprinting genes were evident between hpESCs with higher and lower differentiation potential, except for those in the Dlk1-Dio3 region.

Conclusions: The Dlk1-Dio3 imprinting gene cluster distinguishes the differentiation ability of hpESCs. Moreover, modification by ascorbic acid may facilitate application of hpESCs to clinical settings in the future by enhancing their pluripotency.

语种英语
WOS记录号WOS:000354167800001
项目编号2014CB943203 ; 31371521 ; 81370766 ; 20110001120008 ; 2012 J2200006 ; Yq2013135 ; 2013KJCX0149
资助机构Ministry of Science and Technology of China Grants (973 program) ; National Natural Science Funds for general program ; Ph.D. Programs Foundation of Ministry of Education of China ; Beijing Nova Program ; Zhujiang Science and Technology Star Project of Guangzhou ; Guangdong Province Higher Education Funding
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57595
专题北京大学第三临床医学院_生殖医学中心
北京大学第二临床医学院_麻醉科
北京大学第三临床医学院_妇产科
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
4.Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Major Obstet Dis Guangdong Prov, Guangzhou 510150, Guangdong, Peoples R China
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GB/T 7714
Yu, Yang,Gao, Qian,Zhao, Hong-cui,et al. Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region[J]. STEM CELL RESEARCH & THERAPY,2015,6.
APA Yu, Yang.,Gao, Qian.,Zhao, Hong-cui.,Li, Rong.,Gao, Jiang-man.,...&Qiao, Jie.(2015).Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region.STEM CELL RESEARCH & THERAPY,6.
MLA Yu, Yang,et al."Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region".STEM CELL RESEARCH & THERAPY 6(2015).
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