|Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region|
|Yu, Yang1,2,3; Gao, Qian1; Zhao, Hong-cui1; Li, Rong1,2,3; Gao, Jiang-man1; Ding, Ting1,3; Bao, Si-yu1,2; Zhao, Yue1,3; Sun, Xiao-fang4; Fan, Yong1,4; Qiao, Jie1,2,3|
|刊名||STEM CELL RESEARCH & THERAPY|
|WOS标题词||Science & Technology|
|类目[WOS]||Cell Biology ; Medicine, Research & Experimental|
|研究领域[WOS]||Cell Biology ; Research & Experimental Medicine|
|关键词[WOS]||NUCLEAR TRANSFER ; IN-VITRO ; METHYLATION STATUS ; LINES ; DIFFERENTIATION ; FERTILIZATION ; BLASTOCYSTS ; ACTIVATION ; GENERATION ; CLUSTER|
Introduction: Human parthenogenetic embryonic stem cells (hpESCs) are generated from artificially activated oocytes, however, the issue of whether hpESCs have equivalent differentiation ability to human fertilized embryonic stem cells remains controversial.
Methods: hpESCs were injected into male severe combined immunodeficiency (SCID) mice and the efficiency of teratoma formation was calculated. Then the gene expression and methylation modification were detected by real time-PCR and bisulfate methods.
Results: Comparison of five hpESCs with different differentiation abilities revealed that levels of paternal genes in the Dlk1-Dio3 region on chromosome 14 in the hpESCs with high differentiation potential are enhanced, but strictly methylated and silenced in the hpESCs with lower differentiation potential. Treatment with ascorbic acid, rescued their ability to support teratoma formation and altered the expression profiles of paternally expressed genes in hpESCs that could not form teratoma easily. No differences in the expression of other imprinting genes were evident between hpESCs with higher and lower differentiation potential, except for those in the Dlk1-Dio3 region.
Conclusions: The Dlk1-Dio3 imprinting gene cluster distinguishes the differentiation ability of hpESCs. Moreover, modification by ascorbic acid may facilitate application of hpESCs to clinical settings in the future by enhancing their pluripotency.
|项目编号||2014CB943203 ; 31371521 ; 81370766 ; 20110001120008 ; 2012 J2200006 ; Yq2013135 ; 2013KJCX0149|
|资助机构||Ministry of Science and Technology of China Grants (973 program) ; National Natural Science Funds for general program ; Ph.D. Programs Foundation of Ministry of Education of China ; Beijing Nova Program ; Zhujiang Science and Technology Star Project of Guangzhou ; Guangdong Province Higher Education Funding|
|作者单位||1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China|
2.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
4.Guangzhou Med Univ, Affiliated Hosp 3, Key Lab Major Obstet Dis Guangdong Prov, Guangzhou 510150, Guangdong, Peoples R China
|Yu, Yang,Gao, Qian,Zhao, Hong-cui,et al. Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region[J]. STEM CELL RESEARCH & THERAPY,2015,6.|
|APA||Yu, Yang.,Gao, Qian.,Zhao, Hong-cui.,Li, Rong.,Gao, Jiang-man.,...&Qiao, Jie.(2015).Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region.STEM CELL RESEARCH & THERAPY,6.|
|MLA||Yu, Yang,et al."Ascorbic acid improves pluripotency of human parthenogenetic embryonic stem cells through modifying imprinted gene expression in the Dlk1-Dio3 region".STEM CELL RESEARCH & THERAPY 6(2015).|