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学科主题基础医学
Curcumin inhibits renal cyst formation and enlargement in vitro by regulating intracellular signaling pathways
Gao, Jinsheng; Zhou, Hong; Lei, Tianluo; Zhou, Li; Li, Weidong; Li, Xuejun; Yang, Baoxue1
关键词Curcumin Polycystic Kidney Disease Mdck Cyst Signaling Pathway
刊名EUROPEAN JOURNAL OF PHARMACOLOGY
2011-03-01
DOI10.1016/j.ejphar.2010.12.008
654期:1页:92-99
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]POLYCYSTIC KIDNEY-DISEASE ; EPITHELIAL-CELLS ; FLUID SECRETION ; CYCLIC-AMP ; KAPPA-B ; GROWTH ; PROLIFERATION ; MODEL ; ADPKD ; PATHOGENESIS
英文摘要

Autosomal dominant polycystic kidney disease, a common inherited disease affecting about 1/1000 and 1/400 live births, is characterized by massive enlargement of fluid-filled cysts and eventually causes renal failure. The purpose of this study is to identify the inhibitory effect of curcumin on renal cyst development and to investigate the inhibitory mechanism. Madin-Darby canine kidney (MDCK) cyst model and murine embryonic kidney cyst model were used to evaluate inhibitory activity. Cell viability, proliferation, apoptosis, CFTR function and expression, and signaling pathways in MDCK cells were determined to explore the mechanism of cyst inhibition. Curcumin was found to significantly inhibit MDCK cyst development. At maximum dose curcumin caused 62% inhibition of the cyst formation (IC50 was 0.12 mu M). Curcumin slowed cyst enlargement in both MDCK cyst model and embryonic kidney cyst model with dose-response relationship. Curcumin neither induced cytotoxicity nor apoptosis in MDCK cells at <100 mu M. Curcumin failed to affect the chloride transporter CFTR expression and function. Interestingly, curcumin inhibited forskolin-promoted cell proliferation and promoted the tubule formation in MDCK cells, which indicates curcumin promotes MOCK cell differentiation. Furthermore, curcumin reduced the intracellular signaling proteins Ras. B-raf, p-MEK, p-ERK, c-fos. Egr-1, but increased Raf-1 and NAB2 in MDCK cells exposed to forskolin. These results define that curcumin inhibits renal cyst formation and enlargement and suggest that curcumin might be developed as a candidate drug for polycystic kidney disease. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000287291200014
项目编号30870921 ; 985-2-094-121 ; 7102105
资助机构National Natural Science Foundation of China ; Ministry of Education of China ; Beijing Natural Science Foundation
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57603
专题北京大学基础医学院_药理学系
北京大学基础医学院
作者单位1.Peking Univ, Sch Basic Med Sci, Dept Pharmacol, Beijing 100191, Peoples R China
2.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China
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GB/T 7714
Gao, Jinsheng,Zhou, Hong,Lei, Tianluo,et al. Curcumin inhibits renal cyst formation and enlargement in vitro by regulating intracellular signaling pathways[J]. EUROPEAN JOURNAL OF PHARMACOLOGY,2011,654(1):92-99.
APA Gao, Jinsheng.,Zhou, Hong.,Lei, Tianluo.,Zhou, Li.,Li, Weidong.,...&Yang, Baoxue.(2011).Curcumin inhibits renal cyst formation and enlargement in vitro by regulating intracellular signaling pathways.EUROPEAN JOURNAL OF PHARMACOLOGY,654(1),92-99.
MLA Gao, Jinsheng,et al."Curcumin inhibits renal cyst formation and enlargement in vitro by regulating intracellular signaling pathways".EUROPEAN JOURNAL OF PHARMACOLOGY 654.1(2011):92-99.
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