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学科主题: 临床医学
题名:
Natural autoantibodies against glomerular basement membrane exist in normal human sera
作者: Cui, Z; Wang, HY; Zhao, MH
关键词: natural autoantibodies ; anti-GBM disease ; goodpasture ; autoimmunity ; pathogenesis
刊名: KIDNEY INTERNATIONAL
发表日期: 2006-03-01
DOI: 10.1038/sj.ki.5000135
卷: 69, 期:5, 页:894-899
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Urology & Nephrology
研究领域[WOS]: Urology & Nephrology
关键词[WOS]: IV COLLAGEN ; GOODPASTURES-DISEASE ; ALPHA-3 CHAIN ; FINE SPECIFICITY ; ANTIBODIES ; AUTOIMMUNITY ; AUTOANTIGEN ; REPERTOIRE ; ANTIGEN ; CELLS
英文摘要:

Autoimmunity to glomerular basement membrane (GBM) could induce Goodpasture disease, and natural autoantibodies against GBM in the sera of normal individuals were not reported. The aim of the study was to identify and characterize natural autoantibodies against GBM in normal human sera. Natural anti-GBM autoantibodies were purified from the sera of five healthy persons by affinity chromatography, using purified bovine alpha(IV)non-collagenous (NC1) as solid-phase ligands. Antigen specificity, immunoglobulin G (IgG) subclasses, and antibody avidity of the natural autoantibodies were investigated by enzyme-linked immunosorbant assay (ELISA), Western-blot analysis, indirect immunofluorescence, and antigen-inhibition ELISA, and compared with those of 32 patients with anti-GBM disease. Natural anti-GBM autoantibodies could be purified from IgG fractions of all the five persons, with an average amount of 0.5% of total IgG fractions. Antigen specificity of the natural autoantibodies was identified by blotting to human alpha(IV)NC1, reactivity to recombinant alpha 3(IV)NC1, and linear staining along the GBM of normal kidney sections. Titers of the natural autoantibodies were much lower than those of patients (1:60.6 vs 1: 993.6, P < 0.001). IgG subclasses distribution of the natural autoantibodies was restricted to IgG2 (100%) and IgG4 (100%), while for patients it was mainly IgG1 (93.8%) and IgG4 (90.6%). Avidity of the natural autoantibodies was lower than that of patients, the amount of alpha(IV)NC1 used for 50% inhibition was 1.65 and 0.46 mu g, respectively (P < 0.05). In conclusion, natural anti-GBM autoantibodies exist in normal human sera. Antibody levels, IgG subclasses, and avidity of the natural autoantibodies were different from those of patients. Fine specificity of the natural autoantibodies needed to be elucidated.

语种: 英语
WOS记录号: WOS:000235730000021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57607
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Div Renal, Beijing 100034, Peoples R China
2.Peking Univ, Hosp 1, Inst Nephrol, Beijing 100034, Peoples R China

Recommended Citation:
Cui, Z,Wang, HY,Zhao, MH. Natural autoantibodies against glomerular basement membrane exist in normal human sera[J]. KIDNEY INTERNATIONAL,2006,69(5):894-899.
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