IR@PKUHSC  > 北京大学第二临床医学院  > 肝胆外科
Transfection of CD40Ig into liver prevents acute rejection in rat liver transplantation
Zhu, JY; Liu, YN; Wang, D; Li, T; Leng, XS
WOS标题词Science & Technology
类目[WOS]Immunology ; Surgery ; Transplantation
研究领域[WOS]Immunology ; Surgery ; Transplantation

Objective. To investigate the effect of CD40Ig upon acute rejection of rat liver transplants.

Methods. Thirty-two orthotopic liver transplants were performed using Lewis to BN rats with "the two-cuff technique". The rats were randomly divided into three groups. Group A served as controls (n = 10); group B (n = 11) and group C (n = 11); Lipofectamine2000-pcDNA3.1 or Lipofectamine2000-pcDNA3.1. CD40Ig complex was injected into Lewis portal vein ex vivo before cold storage of the liver. On the fifth day after transplantation, three rats in each group were killed to study the pathological changes and TUNEL immune histochemistry performed to examine CD40Ig expression. Lymphocytes were obtained from the spleen. The mixed lymphocyte reaction (MLR) was performed to determine tolerance and sheep anti-human immunoglobulin G (IgG)-FITC-labeled T cells counted by flow cytometry. Postoperative survival times of rats in each group were recorded. The pathological changes of dead rats were observed.

Results. The mean survival times of group A and B were 11.00 +/- 4.28 and 12.75 +/- 5.57 days, respectively. There were serious acute rejections in allograft liver in groups A and B. Apoptosis index was 33.67 +/- 5.69 versus 39.00 +/- 5.29. Group C mean survival time was 41.25 +/- 13.70 days (P < .01). Immune histochemistry showed CD40Ig-positive elements in the allograft liver, which revealed light acute rejection and apoptosis index was 0.27 +/- 0.21 (P < .01). The part of the allografted liver in a dead rat showed light acute rejection while the others displayed chronic rejection. Recipients were specifically tolerant to donors in the MLR assay. The IgG-FITC-labeled T cells accounted for 11.57% of all T cells in group C.

Conclusions. CD40Ig transfection inhibited T-cell costimulatory pathway, prevented acute rejection, and prolonged graft survival.

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作者单位Peking Univ, Peoples Hosp, Dept Hepatobiliary Surg, Beijing 100044, Peoples R China
Recommended Citation
GB/T 7714
Zhu, JY,Liu, YN,Wang, D,et al. Transfection of CD40Ig into liver prevents acute rejection in rat liver transplantation[J]. TRANSPLANTATION PROCEEDINGS,2005,37(10):4571-4574.
APA Zhu, JY,Liu, YN,Wang, D,Li, T,&Leng, XS.(2005).Transfection of CD40Ig into liver prevents acute rejection in rat liver transplantation.TRANSPLANTATION PROCEEDINGS,37(10),4571-4574.
MLA Zhu, JY,et al."Transfection of CD40Ig into liver prevents acute rejection in rat liver transplantation".TRANSPLANTATION PROCEEDINGS 37.10(2005):4571-4574.
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