IR@PKUHSC  > 北京大学基础医学院  > 生物物理学系
学科主题基础医学
The effects of GM1 on conformational changes of the N-terminus of APP
Ding, JX; Sha, YL; Ruan, Y; Zhu, ZJ; Huang, LX; Geng, HM; Nie, SQ; Zhang, D
关键词Alzheimer&Prime s Disease Amyloid Beta-protein Precursor Ganglioside-gm1 Fluorescence Circular Dichroism
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2003-02-01
30期:1页:107-111
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]AMYLOID PRECURSOR PROTEIN ; ALZHEIMERS-DISEASE ; BETA-PROTEIN ; NEURITE OUTGROWTH ; GROWTH-FACTOR ; BINDING ; MEMBRANES ; DOMAIN
英文摘要

Alzheimer′s disease (AD) is neuropathologically characterized by the presence of extracellular amyloid plaques and intracellular neurofibrillary tangles. The core of senile plaque is amyloid beta-protein (Abeta), which comes from its precursor - amyloid beta-protein precursor (APP). The pQE-APP(28similar to123) plasmids was constructed by gene recombination. The APP(28similar to123) protein was expressed in Escherichia coli and then purified. The purified products were examined for GM1 binding abilities by Western blotting. The effects of GM1 on conformation of APP N-terminus were detected by fluorescence and circular dichroism (CD) techniques. APP(28similar to123) protein could bind with GM1. The intrinsic fluorescence intensity of APP(28similar to123) protein in PC/GM1 vesicles or GM1 solution remarkably increased and the fluorescence peak value blue shifted 20 nm. CD results showed that the major secondary structure of APP(28similar to123) in PBS buffer was alpha-helix. When APP(28similar to123) incubated with PC/GM1 vesicles or GM1 solution, the alpha-helix content increased markedly. These results suggested that GM1 might affect the physiological function of APP and change APP span-membrane process and interfere APP trafficking and internalization by anchoring this molecule on the membrane, which may provide much more substrate for gamma-secretase and enhance Abeta generation on the cells.

语种中文
WOS记录号WOS:000181216300020
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57614
Collection北京大学基础医学院_生物物理学系
作者单位1.Inst Mental Hlth, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biophys, Beijing 100083, Peoples R China
Recommended Citation
GB/T 7714
Ding, JX,Sha, YL,Ruan, Y,et al. The effects of GM1 on conformational changes of the N-terminus of APP[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(1):107-111.
APA Ding, JX.,Sha, YL.,Ruan, Y.,Zhu, ZJ.,Huang, LX.,...&Zhang, D.(2003).The effects of GM1 on conformational changes of the N-terminus of APP.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,30(1),107-111.
MLA Ding, JX,et al."The effects of GM1 on conformational changes of the N-terminus of APP".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 30.1(2003):107-111.
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