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学科主题: 基础医学
题名:
The effects of GM1 on conformational changes of the N-terminus of APP
作者: Ding, JX; Sha, YL; Ruan, Y; Zhu, ZJ; Huang, LX; Geng, HM; Nie, SQ; Zhang, D
关键词: Alzheimer&prime ; s disease ; amyloid beta-protein precursor ; ganglioside-GM1 ; fluorescence ; circular dichroism
刊名: PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
发表日期: 2003-02-01
卷: 30, 期:1, 页:107-111
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: AMYLOID PRECURSOR PROTEIN ; ALZHEIMERS-DISEASE ; BETA-PROTEIN ; NEURITE OUTGROWTH ; GROWTH-FACTOR ; BINDING ; MEMBRANES ; DOMAIN
英文摘要:

Alzheimer′s disease (AD) is neuropathologically characterized by the presence of extracellular amyloid plaques and intracellular neurofibrillary tangles. The core of senile plaque is amyloid beta-protein (Abeta), which comes from its precursor - amyloid beta-protein precursor (APP). The pQE-APP(28similar to123) plasmids was constructed by gene recombination. The APP(28similar to123) protein was expressed in Escherichia coli and then purified. The purified products were examined for GM1 binding abilities by Western blotting. The effects of GM1 on conformation of APP N-terminus were detected by fluorescence and circular dichroism (CD) techniques. APP(28similar to123) protein could bind with GM1. The intrinsic fluorescence intensity of APP(28similar to123) protein in PC/GM1 vesicles or GM1 solution remarkably increased and the fluorescence peak value blue shifted 20 nm. CD results showed that the major secondary structure of APP(28similar to123) in PBS buffer was alpha-helix. When APP(28similar to123) incubated with PC/GM1 vesicles or GM1 solution, the alpha-helix content increased markedly. These results suggested that GM1 might affect the physiological function of APP and change APP span-membrane process and interfere APP trafficking and internalization by anchoring this molecule on the membrane, which may provide much more substrate for gamma-secretase and enhance Abeta generation on the cells.

语种: 中文
WOS记录号: WOS:000181216300020
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57614
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Inst Mental Hlth, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Dept Biophys, Beijing 100083, Peoples R China

Recommended Citation:
Ding, JX,Sha, YL,Ruan, Y,et al. The effects of GM1 on conformational changes of the N-terminus of APP[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2003,30(1):107-111.
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