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Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis
Lu, Q.1,2,3,4; van den Ende, A. H. G. Gerrits1; de Hoog, G. S.1,2,3,5; Li, R.2,3; Accoceberry, I.6; Durand-Joly, I.7; Bouchara, J. -P.8,9; Hernandez, F.10,11; Delhaes, L.10,11
关键词Pseudallescheria Scedosporium Reverse Line Blot Hybridisation Cystic Fibrosis
刊名MYCOSES
2011-10-01
DOI10.1111/j.1439-0507.2011.02108.x
suppl.3期:SI页:5-11
收录类别SCI ; ISTP
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Dermatology ; Mycology
研究领域[WOS]Dermatology ; Mycology
关键词[WOS]SCEDOSPORIUM-APIOSPERMUM INFECTION ; RESPIRATORY-TRACT SPECIMENS ; AIRWAY COLONIZATION ; LUNG TRANSPLANTATION ; SELECTIVE ISOLATION ; FILAMENTOUS FUNGI ; BOYDII COMPLEX ; PREVALENCE ; SAMPLES
英文摘要

The PCR-RLB (reverse line blot hybridisation) was applied as a molecular technique for the detection of members of Pseudallescheria and Scedosporium from sputum of patients with cystic fibrosis (CF). Fifty-nine sputum samples were collected from 52 CF patients, which were analysed by culture and PCR-RLB. Conventional and semi-selective culture yielded five positive samples, but the PCR-RLB hybridisation assay permitted the detection of members of Pseudallescheria/Scedosporium in 32 out of 52 patients (61.5%). In total, PCR-RLB yielded 47 positives. Pseudallescheria apiosperma was detected in 20 samples, while Pseudallescheria boydii and Pseudallescheria aurantiacum were detected in 17 and eight samples, respectively. Six samples gave a positive reaction with two distinct species-specific probes and one sample with three probes. In conclusion, the PCR-RLB assay described in this study allows the detection of Scedosporium spp. in CF sputum samples and the identification of Pseudallescheria apiosperma, P. boydii, S. aurantiacum, Scedosporium prolificans and Pseudallescheria minutispora.

语种英语
WOS记录号WOS:000296262600002
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57636
专题北京大学第一临床医学院_皮肤性病科
作者单位1.CBS KNAW Fungal Biodivers Ctr, NL-3508 AD Utrecht, Netherlands
2.Peking Univ, Dept Dermatol, Hosp 1, Beijing 100871, Peoples R China
3.Peking Univ, Res Ctr Med Mycol, Beijing 100871, Peoples R China
4.Xiangyang Cent Hosp, Dept Dermatol, Xiangyang, Peoples R China
5.Univ Amsterdam, Inst Biodivers & Ecosyst Dynam, Amsterdam, Netherlands
6.CHU Bordeaux, St Andre Hosp, Dept Parasitol & Mycol, Bordeaux, France
7.Hosp Dunkerque, Dept Hyg, Dunkerque, France
8.CHU Angers, Lab Parasitol Mycol, Angers, France
9.Univ Angers, Grp Etud Interat Hote Pathogene, UPRES EA 3132, Angers, France
10.Ctr Hosp Reg & Univ Lille, Dept Mycol, F-59037 Lille, France
11.Univ Lille Nord France, Ctr Infect & Immun, Inserm,Inst Pasteur Lille, CNRS,UMR 7302,BDEEP EA3436,U918, Lille, France
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GB/T 7714
Lu, Q.,van den Ende, A. H. G. Gerrits,de Hoog, G. S.,et al. Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis[J]. MYCOSES,2011,suppl.3(SI):5-11.
APA Lu, Q..,van den Ende, A. H. G. Gerrits.,de Hoog, G. S..,Li, R..,Accoceberry, I..,...&Delhaes, L..(2011).Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis.MYCOSES,suppl.3(SI),5-11.
MLA Lu, Q.,et al."Reverse line blot hybridisation screening of Pseudallescheria/Scedosporium species in patients with cystic fibrosis".MYCOSES suppl.3.SI(2011):5-11.
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