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学科主题: 临床医学
题名:
Dexamethasone affects mouse olfactory mucosa gene expression and attenuates genes related to neurite outgrowth
作者: Tian, Jun1; Pinto, Jayant M.2; Xin, Yi1; Zhang, Henghui3; Li, Li4; Sun, Zhifu1; Wei, Yongxiang1
关键词: corticosteroid use ; olfactory disorders ; olfaction ; microarray ; epithelial cell
刊名: INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
发表日期: 2015-10-01
DOI: 10.1002/alr.21586
卷: 5, 期:10, 页:907-918
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Otorhinolaryngology
研究领域[WOS]: Otorhinolaryngology
关键词[WOS]: UPPER RESPIRATORY-TRACT ; MESSENGER-RNA ; CORTICOSTEROIDS ; MECHANISMS ; RECOVERY ; SYSTEM ; CELLS
英文摘要:

BackgroundOlfaction is one of the important senses for humans. Systemic glucocorticoids are the most commonly used medications for olfactory loss because of their strong anti-inflammatory effects. However, their effect on olfactory function is still controversial and the precise mechanism is not clear. To gain a global view of the effect of systematic glucocorticoid treatment on gene expression in the olfactory mucosa (OM), we profiled these changes in a murine model of olfaction in order to identify underlying molecular mechanisms.

MethodsC57BL/6 mice were injected daily for 2 weeks (WK2) with dexamethasone (DEX, intraperitoneally, 1 mg/kg body weight) vs 1 day of DEX (D1) vs controls, which received saline (Ctrl) (n = 9/group). Total RNA from the OM was used to analyze global gene expression. Genes showing changes in expression were compared using the Database for Annotation, Visualization and Integrated Discovery (DAVID, v6.7) and the General Olfactory Sensitivity Database (GOSdb; ).

ResultsBetween the WK2 and Ctrl groups, 3351 genes were differentially expressed, of which 236 genes were related to olfactory function. Genes involved in axon guidance, cell projection, and inflammation were enriched and overlapped significantly with those in the GOSdb.

ConclusionSystemic glucocorticoids exert effects on transcription of a notable number of genes in the OM and appear to orchestrate changes related to axon guidance, cell projection, and inflammation. Further examination may allow targeted therapies that lack the side effects of this category of medication.

语种: 英语
所属项目编号: 81271062 ; KZ201310025029 ; 2012218048
项目资助者: National Natural Science Foundation, Government of China ; Beijing Natural Science Foundation, Government of China ; Beijing Municipal Science and Technology Commission
WOS记录号: WOS:000363215400006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57640
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Capital Med Univ, Beijing Anzhen Hosp, Dept Otolaryngol Head & Neck Surg, Beijing, Peoples R China
2.Univ Chicago, Dept Surg, Sect Otolaryngol Head & Neck Surg, Chicago, IL 60637 USA
3.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100871, Peoples R China
4.Capital Med Univ, Beijing Chaoyang Hosp, Dept Otolaryngol Head & Neck Surg, Beijing, Peoples R China

Recommended Citation:
Tian, Jun,Pinto, Jayant M.,Xin, Yi,et al. Dexamethasone affects mouse olfactory mucosa gene expression and attenuates genes related to neurite outgrowth[J]. INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY,2015,5(10):907-918.
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