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学科主题临床医学
Both macrophages and hypoxia play critical role in regulating invasion of gastric cancer in vitro
Shen, Zhanlong1,2,3; Kauttu, Tuuli2,3; Seppanen, Hanna2,3; Vainionpaa, Sanna2,3; Ye, Yingjiang1; Wang, Shan1; Mustonen, Harri2,3; Puolakkainen, Pauli2,3
刊名ACTA ONCOLOGICA
2013-05-01
DOI10.3109/0284186X.2012.718444
52期:4页:852-860
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TUMOR-ASSOCIATED MACROPHAGES ; PROSTATE-CANCER ; EXPRESSION ; CELLS ; ANGIOGENESIS ; INFILTRATION ; INFLAMMATION ; PROGRESSION ; CHEMOTAXIS ; ACTIVATION
英文摘要

Background. As previously demonstrated, tumor associated macrophages (TAMs) infiltration is associated with some cancers invasion and metastasis. However, the role of TAMs in the gastric cancer remains unclear. Methods Three-dimensional dynamic migration imaging system and real time RT-PCR were used to quantitatively investigate the effect of macrophages on the cancer cell mobility and gene expression related to cancer invasion and metastasis, including ADAM8, ADAM9, MMP9, TIMP3, VEGF-A and IL8 genes, in AGS, HGC-27, Hs-746T and NCI-N87 gastric cancer cell lines under normal or hypoxic conditions. Results. Under normal conditions, the cancer cell invasion rate was increased significantly and all six gene expressions were upregulated in all four cancer cell lines by macrophages. Under hypoxia the changes in the cancer cell invasion rate induced by macrophages was negatively correlated to the TIMP3 expression. In non-metastatic cell line AGS, the increase in migration rate induced by macrophages was further elevated under hypoxia with increased ADAM8 and ADAM9 expression and decreased MMP9 and TIMP3 expressions. Under hypoxia, the induction by macrophages for IL-8 expression was increased significantly in distant metastatic cell lines NCI-N87 and HS-746T, VEGF-A was increased in HGC-27 cell line. Conclusions. Both macrophages and hypoxia play an indispensable role in regulating the invasion of gastric cancer cells in vitro; ADAMs, MMP9 and TIMP3 might be involved in TAM induced invasive power of gastric cancer cells.

语种英语
WOS记录号WOS:000317239200022
资助机构Sigrid Juselius Foundation ; Helsinki University Central Hospital Research Funds
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被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57642
专题北京大学第二临床医学院_胃肠外科
作者单位1.Peking Univ, Peoples Hosp, Dept Surg Gastroenterol, Beijing 100044, Peoples R China
2.Univ Helsinki, Cent Hosp, Dept Surg, Helsinki, Finland
3.Univ Helsinki, Helsinki, Finland
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Shen, Zhanlong,Kauttu, Tuuli,Seppanen, Hanna,et al. Both macrophages and hypoxia play critical role in regulating invasion of gastric cancer in vitro[J]. ACTA ONCOLOGICA,2013,52(4):852-860.
APA Shen, Zhanlong.,Kauttu, Tuuli.,Seppanen, Hanna.,Vainionpaa, Sanna.,Ye, Yingjiang.,...&Puolakkainen, Pauli.(2013).Both macrophages and hypoxia play critical role in regulating invasion of gastric cancer in vitro.ACTA ONCOLOGICA,52(4),852-860.
MLA Shen, Zhanlong,et al."Both macrophages and hypoxia play critical role in regulating invasion of gastric cancer in vitro".ACTA ONCOLOGICA 52.4(2013):852-860.
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