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学科主题: 临床医学
题名:
Targeted Delivery of GDNF through the Blood-Brain Barrier by MRI-Guided Focused Ultrasound
作者: Wang, Feng1,4; Shi, Yu1; Lu, Lin2; Liu, Li1; Cai, Youli1; Zheng, Hairong3; Liu, Xin3,5; Yan, Fei3; Zou, Chao3,5; Sun, Chengyu2; Shi, Jie2; Lu, Shukun1; Chen, Yun1
刊名: PLOS ONE
发表日期: 2012-12-27
DOI: 10.1371/journal.pone.0052925
卷: 7, 期:12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: NEUROTROPHIC FACTOR ; PARKINSONS-DISEASE ; P-SELECTIN ; RAT-BRAIN ; DRUG ; MICROBUBBLES ; DISRUPTION ; ADDICTION ; MOUSE
英文摘要:

Neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), are promising therapeutic agents for neurodegenerative diseases. However, the application of GDNF to treat these diseases effectively is limited because the blood-brain barrier (BBB) prevents the local delivery of macromolecular therapeutic agents from entering the central nervous system (CNS). Focused ultrasound combined with microbubbles (MBs) using appropriate parameters has been previously demonstrated to be able to open the BBB locally and noninvasively. This study investigated the targeted delivery of GDNF MBs through the BBB by magnetic resonance imaging (MRI)-guided focused ultrasound. Evans Blue extravasation and histological examination were used to determine the optimum focused ultrasound parameters. Enzyme-linked immunosorbent assay was performed to verify the effects of GDNF bound on MBs using a biotin-avidin bridging chemistry method to promote GDNF delivery into the brain. The results showed that GDNF can be delivered locally and noninvasively into the CNS through the BBB using MRI-guided focused ultrasound combined with MBs under optimum parameters. MBs that bind GDNF combined with MRI-guided focused ultrasound may be an effective way of delivering neurotrophic factors directly into the CNS. The method described herein provides a potential means of treating patients with CNS diseases.

语种: 英语
所属项目编号: 30970833 ; 81101048 ; U1204810 ; 2011CB707903 ; 2011M501111 ; JC201005280495A
项目资助者: National Natural Science Foundation ; National 973 basic research program ; National Postdoctoral Science Foundation ; Shenzhen Basic Research Program
WOS记录号: WOS:000312829100100
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57643
Appears in Collections:北京大学深圳医院_超声影像科_期刊论文

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作者单位: 1.Peking Univ, Dept Ultrasound, Shenzhen Hosp, Biomed Res Inst,Shenzhen PKU HKUST Med Ctr, Shenzhen, Peoples R China
2.Peking Univ, Natl Inst Drug Dependence, Shenzhen, Peoples R China
3.Chinese Acad Sci, Paul C Lauterbur Res Ctr Biomed Imaging, Inst Biomed & Hlth Engn, Shenzhen Inst Adv Technol, Shenzhen, Peoples R China
4.Xinxiang Med Univ, Dept Physiol & Neurobiol, Xinxiang, Peoples R China
5.Shenzhen Key Lab MRI, Shenzhen, Peoples R China

Recommended Citation:
Wang, Feng,Shi, Yu,Lu, Lin,et al. Targeted Delivery of GDNF through the Blood-Brain Barrier by MRI-Guided Focused Ultrasound[J]. PLOS ONE,2012,7(12).
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