IR@PKUHSC  > 北京大学深圳医院  > 超声影像科
学科主题临床医学
Targeted Delivery of GDNF through the Blood-Brain Barrier by MRI-Guided Focused Ultrasound
Wang, Feng1,4; Shi, Yu1; Lu, Lin2; Liu, Li1; Cai, Youli1; Zheng, Hairong3; Liu, Xin3,5; Yan, Fei3; Zou, Chao3,5; Sun, Chengyu2; Shi, Jie2; Lu, Shukun1; Chen, Yun1
刊名PLOS ONE
2012-12-27
DOI10.1371/journal.pone.0052925
7期:12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]NEUROTROPHIC FACTOR ; PARKINSONS-DISEASE ; P-SELECTIN ; RAT-BRAIN ; DRUG ; MICROBUBBLES ; DISRUPTION ; ADDICTION ; MOUSE
英文摘要

Neurotrophic factors, such as glial cell line-derived neurotrophic factor (GDNF), are promising therapeutic agents for neurodegenerative diseases. However, the application of GDNF to treat these diseases effectively is limited because the blood-brain barrier (BBB) prevents the local delivery of macromolecular therapeutic agents from entering the central nervous system (CNS). Focused ultrasound combined with microbubbles (MBs) using appropriate parameters has been previously demonstrated to be able to open the BBB locally and noninvasively. This study investigated the targeted delivery of GDNF MBs through the BBB by magnetic resonance imaging (MRI)-guided focused ultrasound. Evans Blue extravasation and histological examination were used to determine the optimum focused ultrasound parameters. Enzyme-linked immunosorbent assay was performed to verify the effects of GDNF bound on MBs using a biotin-avidin bridging chemistry method to promote GDNF delivery into the brain. The results showed that GDNF can be delivered locally and noninvasively into the CNS through the BBB using MRI-guided focused ultrasound combined with MBs under optimum parameters. MBs that bind GDNF combined with MRI-guided focused ultrasound may be an effective way of delivering neurotrophic factors directly into the CNS. The method described herein provides a potential means of treating patients with CNS diseases.

语种英语
WOS记录号WOS:000312829100100
项目编号30970833 ; 81101048 ; U1204810 ; 2011CB707903 ; 2011M501111 ; JC201005280495A
资助机构National Natural Science Foundation ; National 973 basic research program ; National Postdoctoral Science Foundation ; Shenzhen Basic Research Program
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57643
专题北京大学深圳医院_超声影像科
北京大学临床肿瘤学院_核医学科
作者单位1.Peking Univ, Dept Ultrasound, Shenzhen Hosp, Biomed Res Inst,Shenzhen PKU HKUST Med Ctr, Shenzhen, Peoples R China
2.Peking Univ, Natl Inst Drug Dependence, Shenzhen, Peoples R China
3.Chinese Acad Sci, Paul C Lauterbur Res Ctr Biomed Imaging, Inst Biomed & Hlth Engn, Shenzhen Inst Adv Technol, Shenzhen, Peoples R China
4.Xinxiang Med Univ, Dept Physiol & Neurobiol, Xinxiang, Peoples R China
5.Shenzhen Key Lab MRI, Shenzhen, Peoples R China
推荐引用方式
GB/T 7714
Wang, Feng,Shi, Yu,Lu, Lin,et al. Targeted Delivery of GDNF through the Blood-Brain Barrier by MRI-Guided Focused Ultrasound[J]. PLOS ONE,2012,7(12).
APA Wang, Feng.,Shi, Yu.,Lu, Lin.,Liu, Li.,Cai, Youli.,...&Chen, Yun.(2012).Targeted Delivery of GDNF through the Blood-Brain Barrier by MRI-Guided Focused Ultrasound.PLOS ONE,7(12).
MLA Wang, Feng,et al."Targeted Delivery of GDNF through the Blood-Brain Barrier by MRI-Guided Focused Ultrasound".PLOS ONE 7.12(2012).
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Wang, Feng]的文章
[Shi, Yu]的文章
[Lu, Lin]的文章
百度学术
百度学术中相似的文章
[Wang, Feng]的文章
[Shi, Yu]的文章
[Lu, Lin]的文章
必应学术
必应学术中相似的文章
[Wang, Feng]的文章
[Shi, Yu]的文章
[Lu, Lin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。