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学科主题: 基础医学
题名:
Full-genome nucleotide sequence and analysis of a Chinese swine hepatitis E virus isolate of genotype 4 identified in the Guangxi Zhuang autonomous region: evidence of zoonotic risk from swine to human in South China
作者: Li, Lingjun1; Zhu, Yonghong1; Fu, Hongwei1; Wei, Xianfei2; Wang, Ling1; Liang, Jingrui1; Ji, Yanli1; Tang, Ronglan2; Zhuang, Hui1
关键词: full-genome ; genotype ; hepatitis E virus ; South China ; swine ; zoonosis
刊名: LIVER INTERNATIONAL
发表日期: 2009-09-01
DOI: 10.1111/j.1478-3231.2009.02012.x
卷: 29, 期:8, 页:1230-1240
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: CROSS-SPECIES INFECTION ; EASTERN CHINA ; GENETIC-CHARACTERIZATION ; TRANSPLANT RECIPIENTS ; PHYLOGENETIC ANALYSIS ; LENGTH GENOME ; HEV INFECTION ; UNITED-STATES ; WILD BOAR ; PATIENT
英文摘要:

Background

Hepatitis E virus (HEV) is one of the leading causes of the enteric-transmitted acute hepatitis. Many studies have found high identities between human and animal HEV isolates using partial sequence comparison analysis.

Aims

To determine and phylogenetically analyse the complete genome of the swGX40 isolate from the Guangxi Zhuang autonomous region.

Methods

The overlapping fragments of HEV isolate swGX40 were amplified with reverse transcription-nested polymerase chain reaction (PCR) and the 5′ and 3′ ends of viral genome were amplified with rapid amplification of cDNA ends. The PCR products were cloned and sequenced. The sequence and phylogenetic analysis of swGX40 were performed.

Results

The full genome of the swGX40 strain consisted of 7233 nucleotides, excluding the poly (A) tail of 36 residues. There are three open reading frames (ORFs), encoding 1705, 674 and 114 amino acids (aa) respectively. The full-genomic sequencing showed that the swGX40 strain shared similarity with all known HEV genotype 1, 2 and 3 isolates by 73.4-76.5% and with an identity of 83.1-91.2% among genotype 4 HEV isolates. The partial ORF2 sequencing (249 nt) showed that swGX40 shared a high nucleotide identity of 94 and 97% with the Chinese human strain LZ-105 and the Vietnamese human strain HE-JVN-1 respectively.

Conclusions

The swine isolate swGX40 was closely related to the human isolate LZ-105, both of which were collected from Liuzhou, the same district in the Guangxi Zhuang autonomous region. This molecular biological evidence strongly supported the zoonosis hypothesis of hepatitis E.

语种: 英语
所属项目编号: 30570063 ; 2006A A02Z453 ; 0728008
项目资助者: National Natural Science Foundation of China ; 863 National High Technology Research and Development Program of China ; Natural Science Foundation of Guangxi Province
WOS记录号: WOS:000268653200017
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57646
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Liuzhou Med Coll, Dept Microbiol, Liuzhou, Guangxi, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China

Recommended Citation:
Li, Lingjun,Zhu, Yonghong,Fu, Hongwei,et al. Full-genome nucleotide sequence and analysis of a Chinese swine hepatitis E virus isolate of genotype 4 identified in the Guangxi Zhuang autonomous region: evidence of zoonotic risk from swine to human in South China[J]. LIVER INTERNATIONAL,2009,29(8):1230-1240.
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