IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Full-genome nucleotide sequence and analysis of a Chinese swine hepatitis E virus isolate of genotype 4 identified in the Guangxi Zhuang autonomous region: evidence of zoonotic risk from swine to human in South China
Li, Lingjun1; Zhu, Yonghong1; Fu, Hongwei1; Wei, Xianfei2; Wang, Ling1; Liang, Jingrui1; Ji, Yanli1; Tang, Ronglan2; Zhuang, Hui1
关键词full-genome genotype hepatitis E virus South China swine zoonosis
刊名LIVER INTERNATIONAL
2009-09-01
DOI10.1111/j.1478-3231.2009.02012.x
29期:8页:1230-1240
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Gastroenterology & Hepatology
研究领域[WOS]Gastroenterology & Hepatology
关键词[WOS]CROSS-SPECIES INFECTION ; EASTERN CHINA ; GENETIC-CHARACTERIZATION ; TRANSPLANT RECIPIENTS ; PHYLOGENETIC ANALYSIS ; LENGTH GENOME ; HEV INFECTION ; UNITED-STATES ; WILD BOAR ; PATIENT
英文摘要

Background

Hepatitis E virus (HEV) is one of the leading causes of the enteric-transmitted acute hepatitis. Many studies have found high identities between human and animal HEV isolates using partial sequence comparison analysis.

Aims

To determine and phylogenetically analyse the complete genome of the swGX40 isolate from the Guangxi Zhuang autonomous region.

Methods

The overlapping fragments of HEV isolate swGX40 were amplified with reverse transcription-nested polymerase chain reaction (PCR) and the 5′ and 3′ ends of viral genome were amplified with rapid amplification of cDNA ends. The PCR products were cloned and sequenced. The sequence and phylogenetic analysis of swGX40 were performed.

Results

The full genome of the swGX40 strain consisted of 7233 nucleotides, excluding the poly (A) tail of 36 residues. There are three open reading frames (ORFs), encoding 1705, 674 and 114 amino acids (aa) respectively. The full-genomic sequencing showed that the swGX40 strain shared similarity with all known HEV genotype 1, 2 and 3 isolates by 73.4-76.5% and with an identity of 83.1-91.2% among genotype 4 HEV isolates. The partial ORF2 sequencing (249 nt) showed that swGX40 shared a high nucleotide identity of 94 and 97% with the Chinese human strain LZ-105 and the Vietnamese human strain HE-JVN-1 respectively.

Conclusions

The swine isolate swGX40 was closely related to the human isolate LZ-105, both of which were collected from Liuzhou, the same district in the Guangxi Zhuang autonomous region. This molecular biological evidence strongly supported the zoonosis hypothesis of hepatitis E.

语种英语
WOS记录号WOS:000268653200017
项目编号30570063 ; 2006A A02Z453 ; 0728008
资助机构National Natural Science Foundation of China ; 863 National High Technology Research and Development Program of China ; Natural Science Foundation of Guangxi Province
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57646
专题北京大学基础医学院_病原生物学系
作者单位1.Liuzhou Med Coll, Dept Microbiol, Liuzhou, Guangxi, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Li, Lingjun,Zhu, Yonghong,Fu, Hongwei,et al. Full-genome nucleotide sequence and analysis of a Chinese swine hepatitis E virus isolate of genotype 4 identified in the Guangxi Zhuang autonomous region: evidence of zoonotic risk from swine to human in South China[J]. LIVER INTERNATIONAL,2009,29(8):1230-1240.
APA Li, Lingjun.,Zhu, Yonghong.,Fu, Hongwei.,Wei, Xianfei.,Wang, Ling.,...&Zhuang, Hui.(2009).Full-genome nucleotide sequence and analysis of a Chinese swine hepatitis E virus isolate of genotype 4 identified in the Guangxi Zhuang autonomous region: evidence of zoonotic risk from swine to human in South China.LIVER INTERNATIONAL,29(8),1230-1240.
MLA Li, Lingjun,et al."Full-genome nucleotide sequence and analysis of a Chinese swine hepatitis E virus isolate of genotype 4 identified in the Guangxi Zhuang autonomous region: evidence of zoonotic risk from swine to human in South China".LIVER INTERNATIONAL 29.8(2009):1230-1240.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
Full-genome nucleoti(338KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Li, Lingjun]的文章
[Zhu, Yonghong]的文章
[Fu, Hongwei]的文章
百度学术
百度学术中相似的文章
[Li, Lingjun]的文章
[Zhu, Yonghong]的文章
[Fu, Hongwei]的文章
必应学术
必应学术中相似的文章
[Li, Lingjun]的文章
[Zhu, Yonghong]的文章
[Fu, Hongwei]的文章
相关权益政策
暂无数据
收藏/分享
文件名: Full-genome nucleotide sequence and analysis of a Chinese swine hepatitis E virus isolate of genotype 4 identified in the Guangxi Zhuang autonomous region evidence of zoonotic risk from swine to human in South China.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。