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Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array
Ai, JK; Zhang, ZW; Xin, DQ; Zhu, HJ; Yan, QJ; Xin, ZC; Na, YQ; Guo, YL
关键词Suppression Subtractive Hybridization (Ssh) Cdna Microarray Renal Cell Carcinoma (Rcc) Gene Differential Expression
刊名SCIENCE IN CHINA SERIES C-LIFE SCIENCES
2004-04-01
DOI10.1360/03yc0043
47期:2页:148-157
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]TUMOR-TRANSFORMING GENE ; CANCER ; PTTG ; THERAPY ; PROFILES ; PROBES
英文摘要

To isolate the over-expressed genes in human renal cell carcinoma (RCC) and analyze its molecular basis of carcinogenesis, we used the mRNA from human RCC tissues as tester and that from the matched normal kidney tissues as driver to construct the suppression subtractive hybridization library. 379 of the subtracted clones were arrayed onto a nylon membrane and the over-expressed genes were then screened by hybridizing the filter with radioactively labeled cDNA from RCC and matched normal kidney tissues. 67 clones over-expressed in RCC by a factor of 6 or more were sequenced and its identities were analyzed in GenBank database. 4 clones were previously unknown fragments and 2 clones represent KIAA genes. The rest clones were the known genes and some of them were RCC-related, including vascular endothelial growth factor, vimentin and tissue factor. Most of the known genes were the RCC-related genes previously unknown, including zinc ribbon domain-containing 1 protein (ZNRD1), pituitary tumor transforming gene1 (PTTG1). Northern blot and semi-quantitative RT-PCR confirmed that the mRNA levels of the 3 novel fragments and 1 KIAA and 3 known genes were significantly higher in RCC than in the matched normal kidney tissues. Immunohistochemical and Western blot analysis for PTTG1 and ZNRD1 revealed increased protein level in RCC. The over-expressed genes in RCC are the potential molecular targets for diagnosis and therapy and it is very important to understand the molecular mechanism of RCC through the profile of over-expressed genes.

语种英语
WOS记录号WOS:000220726800007
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被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57647
专题北京大学第一临床医学院_泌尿外科
作者单位1.Peking Univ, Inst Urol, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Urol, Beijing 100034, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Physiol, Beijing 100083, Peoples R China
4.Fourth Mil Med Univ, Xijing Hosp, Dept Urol, Xian 710033, Peoples R China
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GB/T 7714
Ai, JK,Zhang, ZW,Xin, DQ,et al. Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2004,47(2):148-157.
APA Ai, JK.,Zhang, ZW.,Xin, DQ.,Zhu, HJ.,Yan, QJ.,...&Guo, YL.(2004).Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,47(2),148-157.
MLA Ai, JK,et al."Identification of over-expressed genes in human renal cell carcinoma by combining suppression subtractive hybridization and cDNA library array".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 47.2(2004):148-157.
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