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学科主题: 临床医学
题名:
Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways
作者: Huang, Chen1,2; Zhang, Pei1; Wang, Wei1; Xu, Yongsheng1,3; Wang, Minshu1; Chen, Xiaoyong1; Dong, Xuran1
关键词: Blue light ; Retinal ganglion cell ; RGC-5 ; Oxidative stress ; Apoptosis ; MAPK
刊名: APOPTOSIS
发表日期: 2014-06-01
DOI: 10.1007/s10495-014-0983-2
卷: 19, 期:6, 页:922-932
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: VISIBLE-LIGHT ; HEME OXYGENASE-1 ; RETINAL CELLS ; DAMAGE ; RAT ; DEGENERATION ; ACTIVATION ; CROSSTALK ; DISEASES ; LINE
英文摘要:

The mechanism of blue light-induced retinal ganglion cell (RGC) injury is poorly understood. In this study, we established a patented light-emitting diode-based system to study the effects of long-term blue light exposure under culture conditions on RGC-5 cells. Long-term blue light exposure significantly reduced cell viability in a time-dependent manner and induced apoptosis and necrosis in RGC-5 cells. Long-term blue light exposure marked an increase in the expression of Bax and active Caspase-3 (p17), which was accompanied by Bcl-2 down-regulation, and displayed features of the mitochondria-dependent apoptosis pathway. Blue light exposure also increased the generation of reactive oxygen species (ROS), and was a strong inducer of ROS-sensitive protein nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression. Moreover, blue light exposure constitutively activated p38 mitogen-activated protein kinases and c-Jun NH2-terminal kinase (JNK), as well as induced the phosphorylation of extracellular signal-regulated kinase in the early phase, in blue light-exposed RGC-5 cells. The protein expression of c-jun and c-fos was further enhanced after RGC-5 cells were exposed to blue light. Taken together, these findings indicated that blue light induced RGC-5 cell line death in dependence upon exposure duration. The potential mechanisms for this phenomenon might be via activated mitochondria-dependent apoptosis, increased ROS production and protein expressions of Nrf2 and HO-1, and activated JNK/p38 MAPK signaling pathways.

语种: 英语
所属项目编号: 81170888
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000334742400002
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57665
Appears in Collections:北京大学第三临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Med Res Ctr, Beijing 100191, Peoples R China
3.Peking Univ, Hosp 3, Clin Stem Cell Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Huang, Chen,Zhang, Pei,Wang, Wei,et al. Long-term blue light exposure induces RGC-5 cell death in vitro: involvement of mitochondria-dependent apoptosis, oxidative stress, and MAPK signaling pathways[J]. APOPTOSIS,2014,19(6):922-932.
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