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学科主题: 临床医学
题名:
Successive alterations of hippocampal gamma-aminobutyric acid B receptor subunits in a rat model of febrile seizure
作者: Han, Y; Qin, J; Bu, DF; Chang, XZ; Yang, ZX
关键词: febrile seizure ; gamma-aminobutyric acid ; receptor ; hippocampus
刊名: LIFE SCIENCES
发表日期: 2006-05-15
DOI: 10.1016/j.lfs.2005.11.023
卷: 78, 期:25, 页:2944-2952
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: TEMPORAL-LOBE EPILEPSY ; CHILDHOOD ABSENCE EPILEPSY ; GABA(B) RECEPTORS ; GENERALIZED EPILEPSY ; EXPRESSION ; GENE ; INHIBITION ; VARIANTS ; PROTEIN ; CLONING
英文摘要:

Febrile seizure (FS) is a frequently encountered seizure type in childhood. Changes of brain function following FS have clinical importance. The recently identified gamma-aminobutyric acid B receptor (GABA(B)R) is a metabotropic receptor of GABA. In this study, we used a rat model of recurrent FS to investigate the changes of GABA(B)R1a and GABA(B)R2 subunits in hippocampus after recurrent FS by using Western blot, quantitative RT-PCR, double immunofluorescence, in situ hybridization and immunoprecipitation/Western blot. After treatment of hyperthermia and the presence of induced seizures once every 2 days for 10 times, GABA(B)R1a and GABA(B)R2 subunits in hippocampus were decreased after 24 h of the last treatment. The decrease of GABABR I a lasted for 15 days but that of GABA(B)R2 persisted for more than 3 0 days. The binding of GABA(B)R1a to GABA(B)R2 in hippocampus was also decreased significantly after 24 h of the last treatment and lasted for more than 30 days. In situ hybridization showed that GABA(B)R1a mRNA was significantly decreased in dentate gyrus, and GABA(B)R2 mRNA was considerably reduced in CA(3) region. In H-10 and FS1 groups in which hyperthermia treatment was the same but no (H-10 group) or only one seizure (FS1 group) was induced, the decrease of GABA(B)R1a and GABA(B)R2 subunits and the reduced binding capability between GABA(B)R1 a and GABA(B)R2 subunits were also detected but with less severity, and the time recovering from these abnormalities was shorter. We conclude that GABA(B)R1a and GABA(B)R2 subunits and the binding of the 2 subunits decrease in hippocampus for a relatively long period of time after recurrent FS in immature rats. These changes may result in long-lasting imbalance of excitation/inhibition function in hippocampus, and are derived from the consequences of recurrent febrile seizures. (c) 2005 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000237749200010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57673
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

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作者单位: 1.Peking Univ, Hosp 1, Ctr Lab, Beijing 100871, Peoples R China
2.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Han, Y,Qin, J,Bu, DF,et al. Successive alterations of hippocampal gamma-aminobutyric acid B receptor subunits in a rat model of febrile seizure[J]. LIFE SCIENCES,2006,78(25):2944-2952.
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