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学科主题: 药学
题名:
Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung
作者: Ding, Wuxiao1; Izumisawa, Tomohiro1; Hattori, Yoshiyuki1; Qi, Xianrong2; Kitamoto, Dai3; Maitani, Yoshie1
关键词: non-ionic surfactant ; mannosylerythritol lipid-A ; Tween 80 ; cationic liposome
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2009-02-01
卷: 32, 期:2, 页:311-315
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: CYSTIC-FIBROSIS ; DELIVERY ; BIOSURFACTANT ; CHOLESTEROL ; NANOPARTICLES ; EFFICIENCY ; VECTORS ; THERAPY ; PATHWAY ; CANCER
英文摘要:

As reported previously, cationic liposomes formulated with dioleoylphosphatidylethanolamine (DOPE) and N,N-methyl hydroxyethyl aminopropane carbamoyl cholesterol (MHAPC-liposomes) achieved efficient gene transfection in the mouse lung following intratracheal injection. We have studied here the role of surfactants, mannosylerythritol lipid-A (MEL-A) and polysorbate 80 (Tween 80), in affecting gene transfection of MHAPC-lipoplexes (complex with pCMV-luc DNA) in A549 cells and in the mouse lung. MEL-A increased gene transfection of MHAPC-lipoplexes significantly in vitro and slightly in the mouse lung, while Tween 80 decreased it both in vitro and in vivo. As assessed by confocal laser scanning microscopy and fluorescence imaging, MEL-A might faciliate gene dissociation from MHAPC-lipoplexes with fluorescein-labeled oligodeoxynucleotide (FITC-ODN) after internalization into the cells and retained the lipoplexes in the mouse lung for prolonged time, while Tween 80 was inefficient to deliver foreign gene into target cells and in the lung. These results demonstrated that MELA is advantageous to Tween 80 in the modification of cationic liposomes as gene delivery vectors in the lung.

语种: 英语
项目资助者: Ministry of Health, Labor and Welfare ; Open Research Center Project
WOS记录号: WOS:000262838800029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57675
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Hoshi Univ, Inst Med Chem, Shinagawa Ku, Tokyo 1428501, Japan
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
3.Natl Inst Adv Ind Sci & Technol, Res Inst Innovat Sustainable Chem, Tsukuba, Ibaraki 3058565, Japan

Recommended Citation:
Ding, Wuxiao,Izumisawa, Tomohiro,Hattori, Yoshiyuki,et al. Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2009,32(2):311-315.
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