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Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung
Ding, Wuxiao1; Izumisawa, Tomohiro1; Hattori, Yoshiyuki1; Qi, Xianrong2; Kitamoto, Dai3; Maitani, Yoshie1
关键词Non-ionic Surfactant Mannosylerythritol Lipid-a Tween 80 Cationic Liposome
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
2009-02-01
32期:2页:311-315
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]CYSTIC-FIBROSIS ; DELIVERY ; BIOSURFACTANT ; CHOLESTEROL ; NANOPARTICLES ; EFFICIENCY ; VECTORS ; THERAPY ; PATHWAY ; CANCER
英文摘要

As reported previously, cationic liposomes formulated with dioleoylphosphatidylethanolamine (DOPE) and N,N-methyl hydroxyethyl aminopropane carbamoyl cholesterol (MHAPC-liposomes) achieved efficient gene transfection in the mouse lung following intratracheal injection. We have studied here the role of surfactants, mannosylerythritol lipid-A (MEL-A) and polysorbate 80 (Tween 80), in affecting gene transfection of MHAPC-lipoplexes (complex with pCMV-luc DNA) in A549 cells and in the mouse lung. MEL-A increased gene transfection of MHAPC-lipoplexes significantly in vitro and slightly in the mouse lung, while Tween 80 decreased it both in vitro and in vivo. As assessed by confocal laser scanning microscopy and fluorescence imaging, MEL-A might faciliate gene dissociation from MHAPC-lipoplexes with fluorescein-labeled oligodeoxynucleotide (FITC-ODN) after internalization into the cells and retained the lipoplexes in the mouse lung for prolonged time, while Tween 80 was inefficient to deliver foreign gene into target cells and in the lung. These results demonstrated that MELA is advantageous to Tween 80 in the modification of cationic liposomes as gene delivery vectors in the lung.

语种英语
WOS记录号WOS:000262838800029
资助机构Ministry of Health, Labor and Welfare ; Open Research Center Project
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57675
专题北京大学药学院_药剂学系
作者单位1.Hoshi Univ, Inst Med Chem, Shinagawa Ku, Tokyo 1428501, Japan
2.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
3.Natl Inst Adv Ind Sci & Technol, Res Inst Innovat Sustainable Chem, Tsukuba, Ibaraki 3058565, Japan
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GB/T 7714
Ding, Wuxiao,Izumisawa, Tomohiro,Hattori, Yoshiyuki,et al. Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2009,32(2):311-315.
APA Ding, Wuxiao,Izumisawa, Tomohiro,Hattori, Yoshiyuki,Qi, Xianrong,Kitamoto, Dai,&Maitani, Yoshie.(2009).Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung.BIOLOGICAL & PHARMACEUTICAL BULLETIN,32(2),311-315.
MLA Ding, Wuxiao,et al."Non-ionic Surfactant Modified Cationic Liposomes Mediated Gene Transfection in Vitro and in the Mouse Lung".BIOLOGICAL & PHARMACEUTICAL BULLETIN 32.2(2009):311-315.
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