IR@PKUHSC  > 北京大学第一临床医学院  > 血液内科
学科主题临床医学
Transcriptional regulation of gamma- and epsilon-globin genes by basic Kruppel-like factor
Ma, XY; Wang, MJ; Qu, XH; Xing, GC; Zhu, YP; He, FC
关键词Bklf Shp1 Reporter Assay
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
2003-03-01
35期:3页:271-276
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]CELL-DIFFERENTIATION ; TYROSINE PHOSPHATASE ; EXPRESSION ; PROMOTER ; ELEMENT ; SHP-1
英文摘要

To study the transcription regulatory function of basic Kruppel-like factor( BKLF) on gamma- and epsilon-globin genes, recombinant expression vectors containing the full-length human BKLF gene, and a deletion mutant that lost N-terminal 40 amino acids, were constructed and used, respectively., to transiently transfect COS7 cells in order to assay their reporter activities. Results showed that hBKLF was able to repress the activity of gamma- and epsilon-globin gene promoters, while the antisense nucleic acid specific for hBKLF activated the transcription of these promoters. Deleting 40 amino acids from N-terminus did not influence the transcriptional repression of hBKLF. The stimulatory function of FKLF on gamma- and epsilon-globin gene promoters was also significantly reduced by hBKLF. In addition, BKLF bound the CACCC element in the SHP1 (SH2-containing protein tyrosine phosphatase 1) gene promoter. These results suggest that gamma- and epsilon-globin genes may be transcriptional targets of BKLF, providing evidence for further studies on the role of BKLF in participating the transcriptional regulation of haemocyte-specific genes.

语种中文
WOS记录号WOS:000181873400010
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/57691
专题北京大学第一临床医学院_血液内科
作者单位1.Beijing Inst Radiat Med, Beijing 100850, Peoples R China
2.Peking Univ, Hosp 1, Dept Hematol, Beijing 100034, Peoples R China
推荐引用方式
GB/T 7714
Ma, XY,Wang, MJ,Qu, XH,et al. Transcriptional regulation of gamma- and epsilon-globin genes by basic Kruppel-like factor[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2003,35(3):271-276.
APA Ma, XY,Wang, MJ,Qu, XH,Xing, GC,Zhu, YP,&He, FC.(2003).Transcriptional regulation of gamma- and epsilon-globin genes by basic Kruppel-like factor.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,35(3),271-276.
MLA Ma, XY,et al."Transcriptional regulation of gamma- and epsilon-globin genes by basic Kruppel-like factor".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 35.3(2003):271-276.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ma, XY]的文章
[Wang, MJ]的文章
[Qu, XH]的文章
百度学术
百度学术中相似的文章
[Ma, XY]的文章
[Wang, MJ]的文章
[Qu, XH]的文章
必应学术
必应学术中相似的文章
[Ma, XY]的文章
[Wang, MJ]的文章
[Qu, XH]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。