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学科主题: 临床医学
题名:
Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification
作者: Yan, Jie2,3; Suzuki, Joao3; Yu, Xiao-Min3; Qiao, Jie2,5; Kan, Frederick W. K.4; Chian, Ri-Cheng1,3
关键词: Oocyte ; Cryo-storage ; Vitrification ; Liquid nitrogen ; Cryo-survival
刊名: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
发表日期: 2011-07-01
DOI: 10.1007/s10815-011-9563-3
卷: 28, 期:7, 页:643-649
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: INTRACYTOPLASMIC SPERM INJECTION ; CRYOPRESERVED HUMAN-EMBRYOS ; DONATION PROGRAM ; BIRTH ; PREGNANCY ; RADIATION ; CYCLES ; BABIES ; ICSI ; MASS
英文摘要:

Purpose To investigate the effects of cryo-storage duration in liquid nitrogen on oocyte cryo-survival, fertilization and embryonic development following vitrification and warming.

Methods Mature mouse oocytes were vitrified with McGill Cryoleaf and stored in liquid nitrogen for a period of 8-10 days, 90-92 days and 180-182 days, respectively. After warming, the survived oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and cultured for 120 h. The rates of oocyte cryo-survival, cleavage and embryonic development were compared.

Result(s) The oocyte cryo-survival rate declined following cryo-storage duration for 180-182 days (90.4 +/- 7.9%) compared to that of the other two groups (97.4 +/- 3.0% and 98.0 +/- 3.3%). The fertilization rate in the group of 180-182 days (66.6 +/- 22.0%) was also significantly reduced (P < 0.05) compared with the groups of 8-10 days (92.2 +/- 10.8%) and 90-92 days (94.7 +/- 9.1%). In addition, the number of embryos developed to the blastocyst stage declined significantly (P < 0.05) following long cryo-storage duration (72.1 +/- 8.2%, 25.2 +/- 3.8% and 5.5 +/- 13.6%, respectively).

Conclusion(s) The cryo-survival, fertilization rate and embryonic development of mouse oocytes are affected significantly, in an adverse manner, by the cryo-storage duration in liquid nitrogen.

语种: 英语
所属项目编号: BMU20090514-603 ; 2007CB948102 ; 2011CB944504
项目资助者: McGill Reproductive Center, McGill University Health Center (MUHC), Montreal, Quebec, Canada ; Peking University ; Major State Basic Research Development Program of the People&prime ; s Republic of China ; Ministry of Science and Technology of China
WOS记录号: WOS:000294353500013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/57717
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Royal Victoria Hosp, Montreal, PQ H3A 1A1, Canada
2.McGill Univ, Dept Obstet & Gynecol, Montreal, PQ H3A 2T5, Canada
3.Queens Univ, Dept Anat & Cell Biol, Kingston, ON, Canada
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
5.Peking Univ, Hosp 3, Med Ctr Human Reprod, Beijing 100871, Peoples R China

Recommended Citation:
Yan, Jie,Suzuki, Joao,Yu, Xiao-Min,et al. Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification[J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,2011,28(7):643-649.
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