|Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification|
|Yan, Jie2,3; Suzuki, Joao3; Yu, Xiao-Min3; Qiao, Jie2,5; Kan, Frederick W. K.4; Chian, Ri-Cheng1,3|
|关键词||Oocyte Cryo-storage Vitrification Liquid Nitrogen Cryo-survival|
|刊名||JOURNAL OF ASSISTED REPRODUCTION AND GENETICS|
|WOS标题词||Science & Technology|
|类目[WOS]||Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology|
|研究领域[WOS]||Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology|
|关键词[WOS]||INTRACYTOPLASMIC SPERM INJECTION ; CRYOPRESERVED HUMAN-EMBRYOS ; DONATION PROGRAM ; BIRTH ; PREGNANCY ; RADIATION ; CYCLES ; BABIES ; ICSI ; MASS|
Purpose To investigate the effects of cryo-storage duration in liquid nitrogen on oocyte cryo-survival, fertilization and embryonic development following vitrification and warming.
Methods Mature mouse oocytes were vitrified with McGill Cryoleaf and stored in liquid nitrogen for a period of 8-10 days, 90-92 days and 180-182 days, respectively. After warming, the survived oocytes were inseminated by intracytoplasmic sperm injection (ICSI) and cultured for 120 h. The rates of oocyte cryo-survival, cleavage and embryonic development were compared.
Result(s) The oocyte cryo-survival rate declined following cryo-storage duration for 180-182 days (90.4 +/- 7.9%) compared to that of the other two groups (97.4 +/- 3.0% and 98.0 +/- 3.3%). The fertilization rate in the group of 180-182 days (66.6 +/- 22.0%) was also significantly reduced (P < 0.05) compared with the groups of 8-10 days (92.2 +/- 10.8%) and 90-92 days (94.7 +/- 9.1%). In addition, the number of embryos developed to the blastocyst stage declined significantly (P < 0.05) following long cryo-storage duration (72.1 +/- 8.2%, 25.2 +/- 3.8% and 5.5 +/- 13.6%, respectively).
Conclusion(s) The cryo-survival, fertilization rate and embryonic development of mouse oocytes are affected significantly, in an adverse manner, by the cryo-storage duration in liquid nitrogen.
|项目编号||BMU20090514-603 ; 2007CB948102 ; 2011CB944504|
|资助机构||McGill Reproductive Center, McGill University Health Center (MUHC), Montreal, Quebec, Canada ; Peking University ; Major State Basic Research Development Program of the People&prime ; s Republic of China ; Ministry of Science and Technology of China|
|作者单位||1.Royal Victoria Hosp, Montreal, PQ H3A 1A1, Canada|
2.McGill Univ, Dept Obstet & Gynecol, Montreal, PQ H3A 2T5, Canada
3.Queens Univ, Dept Anat & Cell Biol, Kingston, ON, Canada
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Beijing 100871, Peoples R China
5.Peking Univ, Hosp 3, Med Ctr Human Reprod, Beijing 100871, Peoples R China
|Yan, Jie,Suzuki, Joao,Yu, Xiao-Min,et al. Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification[J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,2011,28(7):643-649.|
|APA||Yan, Jie,Suzuki, Joao,Yu, Xiao-Min,Qiao, Jie,Kan, Frederick W. K.,&Chian, Ri-Cheng.(2011).Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification.JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,28(7),643-649.|
|MLA||Yan, Jie,et al."Effects of duration of cryo-storage of mouse oocytes on cryo-survival, fertilization and embryonic development following vitrification".JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 28.7(2011):643-649.|